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iTRAQ-based quantitative protein expression profiling of biomarkers in childhood B-cell and T-cell acute lymphoblastic leukemia

Authors Yu R, Zhang J, Zang Y, Zeng L, Zuo W, Bai Y, Liu Y, Sun K, Liu Y

Received 26 March 2019

Accepted for publication 18 June 2019

Published 25 July 2019 Volume 2019:11 Pages 7047—7063

DOI https://doi.org/10.2147/CMAR.S210093

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Ms Justinn Cochran

Peer reviewer comments 2

Editor who approved publication: Dr Teng


Runhong Yu,1 Jingyu Zhang,2 Yuzhu Zang,1 Li Zeng,2 Wenli Zuo,3 Yanliang Bai,1 Yanhui Liu,1 Kai Sun,1 Yufeng Liu4

1Department of Hematology, People’s Hospital of Zhengzhou University/Henan Provincial People’s Hospital, Zhengzhou 450003, Henan, People’s Republic of China; 2Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, People’s Republic of China; 3Department of Hematology, Affiliated Cancer Hospital of Zhengzhou University/Henan Cancer Hospital, Zhengzhou 450008, Henan, People’s Republic of China; 4Department of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, People’s Republic of China

Purpose: This study screened serum proteins to identify potential biomarkers for childhood B-cell and T-cell acute lymphoblastic leukemia (ALL).
Patients and methods: Serum collected from 20 newly diagnosed B-cell ALL, 20 T-cell ALL and 20 healthy children. The peptides from these samples were subjected to iTRAQ. Differentially expressed proteins (DEPs) were further validated by ELISA in 24 B-ALL, 24 T-ALL, and 24 healthy children.
Results: Bioinformatics analysis revealed several pathways, including atherosclerosis signaling, interleukin signaling and production in macrophages and clathrin-mediated endocytosis signaling, that were closely related to childhood T-cell ALL. Furthermore, four selected proteins, namely LRG1, S100A8, SPARC and sL-selectin, were verified by ELISA. These results were consistent with the results of the proteomics analysis.
Conclusion: Serum S100A8 may serve as new diagnostic biomarkers in childhood B-cell ALL and T-cell ALL.

Keywords: B-cell ALL, T-cell ALL, proteomics, acute lymphoblastic leukemia, children, serum, isobaric tags for relative and absolute quantitation, ingenuity pathways analysis

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