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Irisin Protects Against Hind Limb Ischemia Reperfusion Injury

Authors Küçük A, Polat Y, Kılıçarslan A, Süngü N, Kartal H, Dursun AD, Arslan M

Received 2 September 2020

Accepted for publication 18 January 2021

Published 4 February 2021 Volume 2021:15 Pages 361—368

DOI https://doi.org/10.2147/DDDT.S279318

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Qiongyu Guo


Ayşegül Küçük,1,* Yücel Polat,2 Aydan Kılıçarslan,3 Nuran Süngü,3 Hakan Kartal,4 Ali Doğan Dursun,5,* Mustafa Arslan6

1Kutahya Health Sciences University, Medical Faculty, Department of Physiology, Kutahya, Turkey; 2Mehmet Akif Ersoy Thoracic and Cardiovascular Surgery Training and Research Hospital, Department of Cardiovascular Surgery, Istanbul, Turkey; 3Yildirim Beyazit University, Medical Faculty, Department of Pathology, Ankara, Turkey; 4Gulhane Medical Faculty, Gulhane Education and Research Hospital, Department of Cardiovascular Surgery, Ankara, Turkey; 5Atilim University, Medical Faculty, Department of Physiology, Ankara, Turkey; 6Gazi University, Medical Faculty, Department of Anesthesiology and Reanimation, Ankara, Turkey

*These authors contributed equally to this work

Correspondence: Mustafa Arslan
Gazi University, Medical Faculty, Department of Anesthesiology and Reanimation, Ankara, 06510, Turkey
Tel +90 312 202 67 39
Email mustarslan@gmail.com

Aim: The aim of this study was to evaluate the effects of irisin in a murine model of hind limb ischemia reperfusion (I/R).
Methods: The mice were divided into four groups (n = 6 in each group): control, irisin, ischemia reperfusion (I/R), and irisin-ischemia reperfusion (I–I/R). Irisin (0.5 μg.g− 1, intraperitoneally [i.p.]) was administered 30 min before the I/R procedure. After 2 h of ischemia and 2.5 h of reperfusion, blood and tissue samples were taken for biochemical and histopathological analysis. The results were analyzed by Kruskal–Wallis and Mann–Whitney U-tests.
Results: There was a statistically significant difference in the total antioxidant status (TAS) and total oxidant status (TOS) levels in all the groups. The TAS level in the I/R group was significantly lower than that in the control, irisin, and I–I/R groups, whereas the TOS level was significantly higher in the I/R group as compared with that in the other groups. Caspase-3 activity and caspase-8 activity, indicators of inflammation, were significantly higher in the I/R and I–I/R groups as compared with those in the control and irisin groups.
Conclusion: Irisin may have protective effects in skeletal muscle ischemia reperfusion injury.

Keywords: irisin, ischemia reperfusion, caspase-3, caspase-8, mice

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