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Inhibition of Circulating Exosomal miRNA-20b-5p Accelerates Diabetic Wound Repair

Authors Chen K, Yu T, Wang X

Received 22 October 2020

Accepted for publication 30 December 2020

Published 14 January 2021 Volume 2021:16 Pages 371—381

DOI https://doi.org/10.2147/IJN.S287875

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Prof. Dr. Thomas J. Webster


Kai Chen, Tao Yu, Xin Wang

Department of Orthopedic Surgery, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, People’s Republic of China

Correspondence: Xin Wang; Tao Yu
Department of Orthopedic Surgery, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, People’s Republic of China
Tel/Fax +86 21 8572 6525
Email 00848@tongji.edu.cn; yutao247@tongji.edu.cn

Purpose: Efficient approaches to reliably improving wound healing in diabetic patients remain to be developed. Exosomes are nanomaterials from which therapeutically active microRNAs (miRNAs) can be isolated. In the present report, we therefore isolated circulating exosome-derived miRNAs from patients with diabetes and assessed the impact of these molecules on wound healing.
Patients and Methods: Exosomes were isolated from the serum of control or diabetic patients (Con-Exos and Dia-Exos, respectively), after which the effects of these exosomes on cellular activity and wound healing were assessed.
Results: We determined that miR-20b-5p was overexpressed in Dia-Exos and that it functioned by impairing wound repair by suppressing vascular endothelial growth factor A (VEGFA) expression. Consistent with such a model, the administration of Dia-Exos or this miRNA both in vivo and in vitro was sufficient to slow wound repair.
Conclusion: Dia-Exos exhibit significant increases in miR-20b-5p relative to Con-Exos, and this miRNA can be transferred into HSFs wherein it can suppress VEGFA expression and thereby slow the process of wound healing.

Keywords: nanomedicine, exosome, miRNA, diabetes, wound, fibroblast

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