Immunocompromised and immunocompetent mouse models for head and neck squamous cell carcinoma
Authors Sheng S, Ren X, Wang S, Liang X, Tang Y
Received 2 September 2015
Accepted for publication 7 December 2015
Published 27 January 2016 Volume 2016:9 Pages 545—555
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 6
Editor who approved publication: Professor Jianmin Xu
Zhen-ge Lei,1,* Xiao-hua Ren,2,* Sha-sha Wang,3 Xin-hua Liang,3,4 Ya-ling Tang3,5
1Department of Oral and Maxillofacial Surgery, Stomatological Hospital Affiliated to Nanchang University, Nanchang, Jiangxi, 2Department of Stomatology, Sichuan Medical Science Academy and Sichuan Provincial People’s Hospital, 3State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, 4Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, 5Department of Oral Pathology, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, People’s Republic of China
*These authors contributed equally to this work
Abstract: Mouse models can closely mimic human oral squamous epithelial carcinogenesis, greatly expand the in vivo research possibilities, and play a critical role in the development of diagnosis, monitoring, and treatment of head and neck squamous cell carcinoma. With the development of the recent research on the contribution of immunity/inflammation to cancer initiation and progression, mouse models have been divided into two categories, namely, immunocompromised and immunocompetent mouse models. And thus, this paper will review these two kinds of models applied in head and neck squamous cell carcinoma to provide a platform to understand the complicated histological, molecular, and genetic changes of oral squamous epithelial tumorigenesis.
Keywords: head and neck squamous cell carcinoma, HNSCC, mouse models, immunocompromised models, immunocompetent models, transgenic models
Head and neck squamous cell carcinoma (HNSCC) is the sixth most prevalent malignancy in the world and is the third most common cancer in developing countries.1,2 HNSCC affects the nasal cavity and paranasal sinuses, the nasopharynx, oropharynx, and the oral cavity. Oral squamous cell carcinoma (OSCC) develops from the epithelium of the oral cavity, including tongue, lips, gums, floor of the mouth, cheeks, hard palate, or other unspecified parts of the mouth.3 Despite the significant advances made in conventional treatment such as surgery, radiotherapy, chemotherapy, or combinations of these over the past decade, HNSCC continues to have a dismal prognosis, with a 5-year overall and disease-free survival of estimated ~50%.4 Thus, the complicated histological, molecular, and genetic changes of oral and pharynx squamous epithelial tumorigenesis are needed to be thoroughly understood to provide a new therapeutic opportunity and better prognosis for HNSCC patients.
Experimental animal models, which accurately represent the cellular and molecular changes associated with the initiation and progression of human cancer, are thus of crucial importance in the field of cancer research.5,6 Mice are small in size, inexpensive to maintain, reproduce rapidly and genetic engineering can be performed easily compared to other animals.7 Mouse models can closely mimic human oral squamous epithelial carcinogenesis, greatly expand the in vivo research possibilities, and play a critical role in investigating the initiation and development of HNSCC and testing new therapeutic approaches.8 With the development of transgenic technology and emergence of in vivo imaging technology, mouse models could more and more closely simulate the progression of HNSCC than ever before.9,10
Although the molecular mechanisms involved in the association of immunity/inflammation and cancer have remained elusive at present, on the basis of facts that patients with chronic inflammation diseases have a substantially increased risk of developing cancer and that inflammatory cells and immune factors were present in biopsied tumor samples, the role of immunity/inflammation in tumorigenesis has been generally addressed, and the increasing evidence showed that inflammatory/inflammation microenvironment was an essential component of tumors.2,11–14 Hanahan and Weinberg15 have proposed that six hallmarks of cancer together constitute an organizing principle that provides a logical framework for understanding the remarkable diversity of neoplastic diseases in 2000. With the conceptual progression in the following 10 years, two emerging hallmarks, reprogramming energy metabolism and evading immune destruction, were added to the cancer hallmarks list in 2010.16 This strongly highlighted the role of immunity in cancer. Thus, based on the immune state of cancer, this review summarizes immunocompromised and immunocompetent mouse models applied in the research of HNSCC to contribute to our understanding of HNSCC biology (Figure 1).
Figure 1 Mouse models of oral cancer.
In 1960s, a mutation of thymus dysgenesis in mice was discovered, and these mice were called nude mice.17,18 Rygaard and Povlsen19 originally established a human transplanted sigmoid colon adenocarcinoma model by subcutaneously injecting tumor cell suspension into nude mice. In 1983, severe combined immunodeficiency (SCID) mice were reported, which were more immunodeficient than nude mice.20 In SCID mice, there are defects in T- and B-lymphocytes, but the function of natural killer (NK) cells and macrophages is normal. In 1992, an extremely immunodeficient strain of mice was reported by the Jackson Laboratory, which was called nonobese diabetic/SCID (NOD/SCID) mouse.21 NOD/SCID mice not only lacked functional lymphoid cells similar to SCID mice, but also showed reduced activation of NK cells and had less mature macrophage population.22
All these mice were defined as immunocompromised models or xenograft models, which were used to transplant heterologous cells or tissues to establish models systems. To date, these models have been extensively applied in the research of the molecular mechanism of antitumor effect of chemicals on HNSCC, combination of therapies, invasion and metastasis, epithelial–mesenchymal transition, and cancer stem cells (CSCs), especially when in vitro results need to be validated in vivo.
Povlsen and Rygaard23 first reported that three out of four human epidermoid carcinomas at head and neck region were successfully transplanted into nude mice consecutively. Nude mice model for HNSCC research was discussed as early as 198424 and had become the most widely immunocompromised model to be applied in HNSCC. Nude mice transplanted models were used to confirm the inhibitory function of some medicine or factors during tumor development in clinical trials, such as Safrole, BRAK/CXCL14, and doxycycline, which helped to identify the inhibition effect on the growth of HNSCC in vitro.25–27 Using a xenograft model of nude mice, our group found that local hyperthermia acted in an antilymphangiogenic role by suppressing the expression of tumor VEGF-C and VEGF-D, and thereby inhibiting cancer cell lymphatic metastasis in tongue SCC.28 And, we observed that knocking down HIF-1α or HIF-2α individually inhibited the xenograft tumor angiogenesis and growth, and knocking them down simultaneously revealed a better inhibitory effect than knocking down either unit alone.29 In addition, several combinations of therapies, such as lentinan in combination with fluoropyrimidine, radiotherapy in combination with cetuximab, tirapazamine, and MnSOD-plasmid liposome gene therapy, and rapamycin in combination with cisplatin treatment, were shown to be potentially improved strategies for HNSCC in nude mice,30–32 which has identified the role and prospect of the nude mice in studying antitumor effect of chemicals and combination of therapies in HNSCC.
Understanding the molecular mechanisms in the invasion and metastasis of HNSCC is urgently needed for new therapeutic intervention. In 1990, the first mouse model system for metastasis of HNSCC was established by implanting human laryngeal SCC cell lines into the floor of the mouth in nude mice. Pulmonary metastases were noted in 44%, bone invasion in 80%, angioinvasion in 76%, and soft tissue invasion in 96% of the animals.33 Subsequently, various methods were used to build HNSCC mouse models that were prone to metastasis. In 1995, Kawashiri et al34 found that, comparing with the back tumors of nude mice, orthotopic (tongue or the oral floor) tumor exhibited analogous invasive growth and the histological appearance to the original tumors. In this model with OSC-19 cell lines, metastasis to the cervical lymph node was markedly inhibited by cisplatin or peplomycin and the combination of neoadjuvant chemotherapy and tumor resection.35,36 Another experimental model of invasion and metastasis was built by orthotopic transplantation of HSC-2, HSC-3, and HSC-3 cell lines into tongue of nude mice.37
Bone invasion attracted great attention as a common behavior of HNSCC, which always caused facial defects and dysfunction of mandibular and maxillary bone and affected the prognosis. By injecting UM-SCC-1 cell lines into mylohyoid muscle of nude mice, the investigator got a model which allowed quantification of tumor invasion.38 In order to make another bone invasion model, human OSCC cell lines (SCC14a, SCC1, and SCC12) were injected into the surface of calvaria in NCr-nu/nu athymic mice. A higher tumor growth and increased rate of osteolysis were found in SCC14a tumor bearing mice, which was related to high level production of CXCL13.39 These mouse models with metastasis aimed to elucidate the molecular mechanism of the invasion and metastasis in HNSCC.
Yadav et al,40 through transplanting head and neck tumor cell line (CAL27) into SCID mice, showed that interleukin-6 (IL-6) was a promoter of HNSCC tumor metastasis and worked by inducing epithelial–mesenchymal transition via the JAK-STAT3-SNAIL signaling pathway. And Shinriki et al41 investigated the inhibitory effects of anti-IL-6 receptor antibody tocilizumab on OSCC growth using SCID mice xenograft model based on in vitro data. In another experiment, Jung et al42 found keratinization-associated miR-7 and miR-21 deregulated tumor suppressor reversion-inducing cysteine-rich protein with kazal motifs in oral cancer which led to aggressiveness of tumors with CAL27 orthotopic xenograft mouse model. Yang et al43 inoculated connective tissue growth factor (CTGF)-overexpressed SAS cells (SAS/CTGF-M3) into buccal mucosa of SCID mice to establish OSCC model, showing reverse correlation between CTGF and miR-504, miR-504 and FOXP1, and a positive correlation between CTGF and FOXP1. Taken together, the SCID mouse model provides an avenue to analyze OSCC-associated mediators or study it as a potential therapeutic target for OSCC.
Although NOD/SCID mice model was used in research on hematopoietic and lymphatic system at the beginning, it was gradually employed for studying solid tumors. With bioluminescent imaging technology, Lin et al44 injected human transfected SAS/luc cells into NOD/SCID mice, established an OSCC model, and observed a significant inhibition effect of curcumin on tumor growth. Patel et al45 found that rapamycin and the rapalog RAD001 diminished lymphangiogenesis in the primary tumors and prevented the dissemination of HNSCC cells to the cervical lymph nodes, thereby prolonging NOD/SCID mice survival, suggesting that mTOR was a potential target of HNSCC. Recently, the NOD/SCID mouse model has been regarded as the gold standard assay for defining CSCs fraction.46,47 With a NOD/SCID mouse model, Prince et al48 tested the tumorigenic potential of different populations of cancer cells derived from primary, unmanipulated human HNSCC samples and found that the CD44+ population possessed the unique properties of self-renewal and differentiation. Clay et al49 implanted high aldehyde dehydrogenase (ALDHhigh) activity and ALDHlow populations of HNSCC cells into NOD/SCID mice and confirmed that ALDH activity was a highly selective CSCs marker for HNSCC. In a NOD/SCID xenograft model derived from clinical HNSCC specimens, Sun and Wang50 injected sorted cells subcutaneously and found that c-Met+ cells were capable of self-renewal and generating tumors and that isolation of HNSCC cells using a second marker CD44 could further enhance the in vivo tumorigenicity.
Recently, NOD SCID gamma (NSG) or NOD/SCIDIL-2rγnull (NOG) mice have been established, and SCID mice are now subdivided into the NOD/SCID mice and NOG mice. The NOG mouse model combines the features of SCID and IL-2 receptor gamma chain deficiency. Analogously to the NOD/SCID mice, these mice lack mature T-cells, B-cells, and macrophages. For the detection of common cytokine receptor, γ-chain, this model, which is characterized by extremely low number of NK cells and dysfunctional dendritic cells, is used. As a result, NOG mice had no functional lymphocytes and efficacious NK activity. Hence, NOG mice with most severe degree in the deficiency of immunity are characterized by high xenograftment efficiency, even only a few cells and almost no rejection of human cells and tissues. Although extremely immunodeficient, NOG mice can live for more than 1½ years under strict specific pathogen-free condition. Compared to NOD/SCID mice, the “leakiness” of T- and B-cells does not occur in NOG mice, though the reason remains to be elucidated. Currently, NOG mice have been used to examine hematopoietic stem cells (HSCs), identify CSCs, and research the embryonic stem cells and induced pluripotent stem cells. These mice also have been applied as models to study cancers and infectious diseases such as HIV-1 infection. Besides, NOG mice can be a used as a platform to develop a new humanized mice model. Shrivastava et al51 established tumor xenografts by direct injection of cells into the flanks of NSG mice to examine the ability to form tumor from parental and oralspheres of OSC19 cells in NSG mice. The result showed that oralspheres contributed to tumor growth and vascularization, indicating that oralspheres might be future therapeutic modalities against treatment resistant HNSCC.51
Although the immunocompromised mouse models preventing host rejection were widely applied in HNSCC studies because of their certainty of tumor initiation, speed of tumor development, and easy establishment (injecting cells or transplant tissues subcutaneously or orthotopically in animal), there are still some disadvantages of immunocompromised models. The rate of invasion and metastasis is very uncertain and different, changing from very low to markedly high due to dissimilar transplantation methods, hosts, and transplantation site, though invasion or metastasis was observed in most cases.33,34,37 One hundred and six malignant human tumor cell lines including 11 different tumor cell lines were studied in 1,045 nude mice, with only 14 (1.3%) instances of metastasis.52 In addition, some studies have showed that many compounds with significant antitumor effects in immunocompromised models do not work in human beings.53 Due to the totally different tumor immune microenvironment in immunodeficient mice compared with humans, it is difficult to evaluate the interaction between treatment and immune system by using this kind of model. In a word, the inability to simulate the in vivo inflammation/immunity environment could be the Achilles’ heel of immunocompromised mouse models for HNSCC.
The development of immunodeficient mice provides the possibility of establishing humanized mice (mouse models with humanized immune systems, which carry partial or complete human physiological systems), representing ground-breaking platforms to evaluate compounds to treat cancer.54 Immunodeficient mice were engrafted with human HSCs or human peripheral blood mononuclear cells, and exogenous administrations of human hormones, growth factors, and cytokines were all needed. Humanized CD34+ mice were produced by injecting CD34+ and yielded robust multilineage immune systems with good T-cell maturation and function for long-term studies. This model has the longest research span, over 12 months with a functional human immune system, and displays T-cell dependent inflammatory responses, with no donor cell immune reactivity toward host.55,56 However, the application of humanized CD34+ mice in HNSCC has not been reported till now and should be gradually employed in the future.
Humanized mice, one kind of spontaneous HNSCC model, is extremely similar to human tumors and has a high value in studying effects of environmental factors, carcinogenic factors, and genetic factors in tumorigenesis, especially in the immunocompetent environment. However, many animals and much time are needed to establish this kind of model, and it is difficult to reproduce this model.57 Due to these limitations, spontaneous HNSCC model can hardly be employed widely.
Chemically induced models
Initially, inducing oral malignant tumors chemically always failed, because the oral mucosa was more resistant to the action of chemicals than skin.58 The most common explanations for the difference in susceptibility were sebaceous glands and hair follicles in the skin, and the influence of saliva in the mouth.59 Finally, HNSCC was successfully induced in hamster cheek pouch as an animal model, using 9, 10 dimethyl-1, 2, benzanthracene (DMBA), by Salley.60 In 1961, Morris61 standardized this experimental model. In general, histologically, the carcinogenesis of mucosa was divided into four successive stages: hyperplasia, atypical hyperplasia, carcinoma in situ, and squamous cell carcinoma. However, there was difficulty in distinguishing between the changes of the epithelium caused by direct contact with the carcinogen and true premalignant transformation because they were transient and reversible in the DMBA-induced cheek tumors. DMBA-induced tumors did not possess many of the histological features of differentiated SCC and did not closely resemble early human lesions.62,63 More importantly, the tumor occurred in the hamster cheek pouch, which was an immunodeficient area and is absent in human, so this model did not mimic human OSCC very well.
Although DMBA was widely employed in hamster and rat oral cancer models,60,61,64 it proved to be difficult to induce oral carcinoma with DMBA in mice models.65 4-Nitroquinoline 1-oxide (4-NQO), a water-soluble quinolone derivative, was then introduced to OSCC model. Administration of 4-NQO with drinking water or a topical application produced a spectrum of carcinogenesis progression that demonstrated multiple dysplastic, preneoplastic, and neoplastic lesions after long-term treatment in both rat and mouse models, and these could mimic human oral cavity neoplastic transformation.66–72 4-NQO-induced mouse model of oral cancer has been gradually adopted and improved. In early research, 4-NQO was painted on the surface of oral mucosa, but this method was laborious and difficult in terms of applying the same dose from one mouse to another.66 Furthermore, the progress of carcinogenesis was not as natural as in human. Drinking water administration method was then introduced. This model and method was standardized by Tang et al.67 In Tang et al’s study,67 4-NQO painted tongues or delivery of 4-NQO in the drinking water were compared in both CBA mice and C57BL/6 mice, and the latter one was found with a much higher incidence of oral cavity carcinogenesis. The concentration of 4-NQO in drinking water (100 μg/mL) and 16 weeks of treatment have been widely adopted in other research that followed.
Many features of human oral cancer development have been reflected in the 4-NQO mice model. With this model, the molecular mechanisms involved in the initiation and progression of HNSCC can be thoroughly comprehended. A research about epithelial–mesenchymal interactions indicated that the underlying connective tissue adjacent to the epithelium was also affected in the later stages of carcinogenesis.73 DNA hypomethylation was shown to suppress oral squamous cell carcinogenesis in 4-NQO mice model.74,75 It was reported that angiogenesis inhibited by ABT-510 could slow or delay the development of HNSCC.76 Inactivation of VEGFR or EGFR could significantly suppress oral cancer development and progression in 4-NQO mouse model.77–79 These results could be the clues that would help to find therapeutic target for HNSCC.
Syngeneically transplanted models
Traditionally, human tumor xenograft models were established by injecting tumor cell lines or transplanting tissues from patients into immunocompromised mouse. But this model was not suitable to dissect the role of tumor and host immunity factor in malignant transformation and metastatic tumor progression of OSCC. Syngeneic transplanted model was achieved by injecting homologous cell lines into immunocompetent mice, with the advantages of easily forming tumor and avoiding the host rejection in immunocompetent environment.80
In 1984, with injecting SCCVII/SF cells cultured in vitro subcutaneously into syngeneic C3H/HeJ mice, a syngeneic transplanted model was created. Subsequently, positive effects were obtained in the study that combined continuous low dose rate irradiation and concurrent infusion of bleomycin, cyclophosphamide, cis-platinum, 5-fluorouracil, actinomycin D, and mitomycin C, which is chemoradiotherapy.81 By injecting the same cell line into the floor of the mouth of C3H/HeJ mice, O’Malley et al82 established an OSCC model and presented local invasion and pulmonary metastases. Another oral cancer model was built by implanting AT-84 (a spontaneously arising OSCC of C3H mice) cell line into syngeneic mice to study the mechanisms involved in immune-mediated therapy of HNSCC.83 Cell line derived from 4-NQO-mediated tumorigenic SCID mice was inoculated into BALB/c and congenic SCID mice to determine whether CD80 expression affected the immune response and therapy in OSCC.84 In this syngeneic mouse model, the combined use of IL-12 and IL-2 resulted in significantly greater tumor reduction than with either one alone.85 In Meth-A fibrosarcoma-bearing BALB/c mice or SCCVII-bearing C3H/HeN mice, local dendritic cell therapy with concurrent administration of TS-1 and OK-432 was a useful strategy for the treatment of solid tumors. And the potential mechanism was related to TLR4 signaling.86
Within the immunocompetent environment, syngeneic transplanted models possess better application prospects for investigating the mechanism of OSCC invasion and metastasis. Cui et al and Nomura et al87,88 inoculated SCCVII cells into masseter region of C3H/HeN mice to establish a bone invasion model. They found that various cancer-derived cytokines, such as IL-6, PTHrP, TNF-α, RANK, RANKL, osteoproteger, played important roles in the OSCC invading bone, and by inhibiting these cytokines, YM529 (a third-generation bisphosphonate) could suppress osteoclast-mediated bone invasion. A similar model was also used by Lee et al89 and Furuta et al.90 In another research, MDA1986 cell lines were inoculated into tongue orthotopically; however, the incidence of regional and systemic metastases was too low. To increase cell line metastatic potential, Myers et al91 isolated tumor cells from cervical lymph nodes and performed serial passage of these lymph nodes. Those cells were more metastatic than their parental line, with 30% developing regional lymph nodes metastasis, and 20%, lung metastasis. By the same method, the 686LN cell line was injected into the submandibular to mylohyoid muscle of nude mice and a high metastatic cell line (686LN-M2) with a metastasis rate of 50% was established.92 Chen et al93 injected murine SCC cell line (Pam) into BALB/c SCID mice, forming tumors. They then separated tumor cells, injected them into immunocompetent BABL/c mice, resulting in tumor and lymph node metastasis. Cell line selected from the lymph node exhibiting a stronger metastatic ability was named as Pam-LY.
Genetically engineered model
In 1984, transgenic mouse model was originally utilized in mammary adenocarcinomas and brain tumors studies by Stewart et al94 and Brinster et al.95 Transgenic and knockout technology can upregulate or downregulate the expression of certain genes, even silence it. So, we can evaluate the effect of the target gene in the origination and progression of HNSCC in immunocompetent environment.
Ras as an oncogene, frequently activated by point mutations or overexpression, plays a crucial role in human tumors. It has been widely used to establish novel mouse models of HNSCC. In 1987, Sinn et al96 mated two separate strains of transgenic mice that carry the v-Ha-ras and c-myc, respectively, which were both driven by the mouse mammary tumor virus (MMTV) promoter/enhancer to yield hybrid mice, and resulted in a dramatic and synergistic acceleration of mammary and salivary tumor formation. A similar model has been employed extensively because of its high susceptibility to the development of mammary and salivary tumors.96–101 Crossing v-Ha-ras gene (+) FVB/n mice with HPV16 E6/E7(+) C57BL/6 mice, Schreiber et al102 observed a remarkable synergy between these two oncogenes in the development of primary mouth, eye, and ear tumors in mice. Using K5-rTA, tet-ras, and tetO-LacZ transgenic mice, Raimondi et al103 proved that inducing expression of K-ras gene under the control of K5 promoter could trigger a full carcinogenesis. In addition, they successfully established an oral-specific K-ras and p53 two-hit carcinogenesis model system.104 Using a similar model, Van der Weyden et al105 demonstrated that the activated oncogenic K-ras in the mouse oral mucosa could induce the formation of OSCC. To elucidate the effect of ras mutations in oral cancer formation, Caulin et al106 created a novel model that allowed the focal activation of K-ras in stratified epithelia. In a H-ras mutation model, male CB6F1-Tg-rasH2 @Jcl mice, Miyamoto et al107 demonstrated that 4-NQO more easily induced tongue and esophageal carcinogenesis.
The p53 protein plays a pivotal role in inducing cell cycle arrest or apoptosis in the presence of DNA damage. A series of transgenic mouse model systems has been created for the sake of understanding the role and mechanism of p53 in the HNSCC occurrence and development. A type of dominant-negative p53 transgenic mouse was shown to be highly susceptible to 4-NQO with higher prevalence and more rapid development of OSCC.108 Moral et al109 had produced a mouse model that combined constitutive Akt activity (myrAkt) with tumor repressor protein 53 (Trp53) loss and found that it was prone to develop HNSCC. In xeroderma pigmentosum group A gene-deficient (XPA−/−) mouse strain carrying mutant alleles for p53, treated with 4-NQO, Ide et al110 found the accelerated tongue tumor growth might be a consequence of haplonin sufficiency rather than the mutation of p53 in the context of nucleotide excision repair deficiency. In order to understand the role of p53 in the responses of chemotherapy, Bearss et al and Petit et al100,101 used MMTV-ras transgenic mice to interbreed to p53 knockout mice, and then created a model. In a p53 loss with c-neu overexpression mice model, Brodie et al111 have observed the increased rate of parotid gland tumor onset and no effects on breast tumor formation.
Transforming growth factor-beta (TGF-β), a key regulator of epithelial cell proliferation, immune function, and angiogenesis, is widely studied in human tumors, including HNSCC.112 Hyperproliferation, reduced apoptosis, and increased genomic instability are related to defective TGF-β signal in epithelial cells.113,114 In order to define the role of TGF-βRII in HNSCC, Liu et al115 established a TGF-βRII-deleted mouse model. Activation of either K-ras or H-ras in combination with TGF-βRII deletion from mouse head-and-neck epithelia caused HNSCC with complete penetrance, some of which progressed to metastases. With the same model system, their further findings demonstrated that the abrogation of TGF-β signaling would continuously stimulate NF-κB, and then result in malignant progression.116 Bian et al117 developed an inducible mouse model by knocking out Tgf-βR1 and Pten. In this model, NF-κB pathway activation, myeloid-derived suppressor cell infiltration angiogenesis, and immune suppression in the tumor microenvironment were considered to contribute to head and neck carcinogenesis, which indicated that the tumor microenvironment played important role in the HNSCC.117 In the same mouse model, by inhibiting mTOR signal, chemopreventive rapamycin treatment significantly delayed the onset of the HNSCC and prolonged survival of mice.118,119 PI3K-mTOR inhibition can enhance TP53/p73 expression and significantly inhibit tumor growth alone.120 In summary, mouse models with TGF-β signaling pathway may be quite useful for the research of HNSCC treatment.
High-risk human papillomaviruses (HPVs) are linked etiologically to a subset of HNSCC.121 Jabbar et al122 established a model for HPV-associated HNSCC in which they treated transgenic mice expressing the papilloma viral oncoproteins with 4-NQO. They found that the HPV-16 E7 oncoprotein was highly potent at causing HNSCC. They further discovered a striking synergy between E6 and E7 in causing HNSCC.122 To study the biological activities of the HPV 16 E6 and E7 genes in epithelial cells in vivo, transgenic mice with HPV 16 E6 and E7 were established and tumorigenicity by HPV 16 E6 and E7 was obtained.123,124 Further, Song et al125 have used transgenic mice that express HPV 16 E6 or E7 in the epidermis to find the mechanism by which HPV 16 E6 or E7 abrogates radiation-induced DNA damage responses in vivo through p53-dependent and p53-independent pathways.125 Nguyen et al126 generated transgenic mice, K14E6 (Delta 146–151), that express stratified squamous epithelia from the human keratin 14 (K14) promoter. These mice exhibit a radiation response similar to that of the K14E6 (wild-type [WT]) mice, demonstrating that HPV16 E6 retains an ability to inactivate p53.126–128 And in this model, E6 has been showed to primarily contribute to the late stages of carcinogenesis that lead to malignancy.129,130
Other cancer associated genes, such as COX-2, MMP-8, XPA, cyclin D1, and PLAG1, have been utilized to establish models. Zinc-deficient mice with genetic COX-2 deletion developed significantly greater upper aerodigestive tract tumor than WT controls, and presented strong LTA(4)H immunostaining in induced tumors.131 The data from MMP-8 knockout mice model system implied that MMP-8 was a protective factor in the growth of mobile tongue SCC.132 By assessing the response of XPA−/− mice to 4-NQO, the nucleotide excision repair gene XPA was considered as a defensive factor against this carcinogen in vivo.133 Cyclin D1 overexpression mice were crossed with p53-deficient mice, then a well-defined model of oral–esophageal cancer was successfully established. It could be a platform for genome-wide analysis, which was found to be useful to test the chemopreventive, diagnostic, and therapeutic strategies.134 In PLAG1 transgenic mice, Declercq et al135 found that pleomorphic adenomas of the salivary glands were induced and that both Igf and Wnt signaling pathway were involved.
Experiments in vivo with genetically engineered models have greatly advanced the understanding of tumorigenesis because of interventions at gene level. However, the transgenic model is still not a perfect model. Gene mutations are present in all the cells of transgenic animal since embryogenesis, whereas mutations only occur in few cells surrounded by normal cells rather than generalized cells in humans. This promotes the building of the targeted and inducible models on which gene mutation in the targeted cells of animals can be generated. Andl et al136 developed two mouse models targeting ablation of Cdh1 and Tgfbr2 genes constitutively or inducibly in the oral–esophageal epithelium, which had long latency and could phenocopy the human disease quite accurately. Opitz et al137 generated L2-cyclin D1 (L2D1(+)) mice and crossbred these with p53-deficient mice; L2D1(+)/p53(+/−) mice provided a well-defined, novel, and faithful model of oral–esophageal cancer.137 Stairs et al138 have generated a conditional knockout model of p120ctn, whereby mice develop preneoplastic and neoplastic lesions in the oral cavity, esophagus, and squamous forestomach. The targeted and inducible models can avoid gene mutation of animal cells, and this model should be widely popularized in the future research of HNSCC.
Conclusion and perspectives
Mouse models play an important role in HNSCC by providing a model to identify the markers for early diagnosis, understanding the mechanism of tumor initiation and development, and studying the prevention and treatment of HNSCC. There are many different model systems for HNSCC; however, the results obtained from different models may be quite different and dissatisfying to some extent. A real ideal model, which can phenocopy HNSCC progression perfectly, has not been established so far. Each model system has its advantages and disadvantages. We cannot use one key to open all the doors, just as one mouse model cannot be used to study all cancers. Cocarcinogenesis may have a better strategy, and this means using more than one method in one mouse model system in order to minimize the disadvantages and make the model more effective. With the development of technology and the enrichment of knowledge and understanding, more perfect HNSCC mouse models could be hopefully established to improve research on HNSCC and help to better treatment eventually.
This work was supported by National Natural Science Foundation of China grants (numbers 81361120399, 81321002, 81272961, 81372891, and 81572650), Fundamental Research Funds of the Central Universities of China (2015), and by State Key Laboratory of Oral Diseases Special Funded Projects (SKLOD201512).
The authors report no conflicts of interest in this work.
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