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ICG-Loaded PEGylated BSA-Silver Nanoparticles for Effective Photothermal Cancer Therapy

Authors Park T, Lee S, Amatya R, Cheong H, Moon C, Kwak HD, Min KA, Shin MC

Received 1 April 2020

Accepted for publication 8 July 2020

Published 31 July 2020 Volume 2020:15 Pages 5459—5471

DOI https://doi.org/10.2147/IJN.S255874

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Anderson Oliveira Lobo


Taehoon Park1 ,* Sumi Lee2 ,* Reeju Amatya,1 Heesun Cheong,3 Cheol Moon,4 Hyun Duck Kwak,5 Kyoung Ah Min,2 Meong Cheol Shin1

1College of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju, Gyeongnam 52828, Republic of Korea; 2College of Pharmacy and Inje Institute of Pharmaceutical Sciences and Research, Inje University, Gimhae, Gyeongnam 50834, Republic of Korea; 3Division of Cancer Biology, National Cancer Center, Goyang, Gyeonggi-do 10408, Republic of Korea; 4College of Pharmacy, Sunchon National University, Suncheon, Jeonnam 57922, Republic of Korea; 5Department of Ophthalmology, Busan Paik Hospital, Inje University College of Medicine, Busanjin-gu, Busan 47392, Republic of Korea

*These authors contributed equally to this work

Correspondence: Kyoung Ah Min
College of Pharmacy and Inje Institute of Pharmaceutical Sciences and Research, Inje University, Gimhae, Gyeongnam 50834, Republic of Korea
Tel +82 55 320 3459
Fax +82 55 320 3940
Email minkahh@inje.ac.kr
Meong Cheol Shin
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju, Gyeongnam 52828, Republic of Korea
Tel +82 55 772 2421
Fax +82 55 772 2429
Email shinmc@gnu.ac.kr

Purpose: Indocyanine green (ICG), a near infrared (NIR) dye clinically approved in medical diagnostics, possesses great heat conversion efficiency, which renders itself as an effective photosensitizer for photothermal therapy (PTT) of cancer. However, there remain bottleneck challenges for use in PTT, which are the poor photo and plasma stability of ICG. To address these problems, in this research, ICG-loaded silver nanoparticles were prepared and evaluated for the applicability as an effective agent for photothermal cancer therapy.
Methods and Results: PEGylated bovine serum albumin (BSA)-coated silver core/shell nanoparticles were synthesized with a high loading of ICG (“PEG-BSA-AgNP/ICG”). Physical characterization was carried out using size analyzer, transmission electron microscopy, and Fourier transform infrared spectrophotometry to identify successful preparation and size stability. ICG-loading content and the photothermal conversion efficiency of the particles were confirmed with inductively coupled plasma mass spectrometry and laser instruments. In vitro studies showed that the PEG-BSA-AgNP/ICG could provide great photostability for ICG, and their applicability for PTT was verified from the cellular study results. Furthermore, when the PEG-BSA-AgNP/ICG were tested in vivo, study results exhibited that ICG could stably remain in the blood circulation for a markedly long period (plasma half-life: 112 min), and about 1.7% ID/g tissue could be accumulated in the tumor tissue at 4 h post-injection. Such nanoparticle accumulation in the tumor enabled tumor surface temperature to be risen to 50°C (required for photo-ablation) by laser irradiation and led to successful inhibition of tumor growth in the B16F10 s.c. syngeneic nude mice model, with minimal systemic toxicity.
Conclusion: Our findings demonstrated that PEG-BSA-AgNPs could serve as effective carriers for delivering ICG to the tumor tissue with great stability and safety.

Keywords: silver nanoparticle, bovine serum albumin, indocyanine green, photothermal therapy, cancer

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