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How CRISPR-Cas System Could Be Used to Combat Antimicrobial Resistance

Authors Gholizadeh P, Köse Ş, Dao S, Ganbarov K, Tanomand A, Dal T, Aghazadeh M, Ghotaslou R, Ahangarzadeh Rezaee M, Yousefi B, Samadi Kafil H

Received 25 January 2020

Accepted for publication 31 March 2020

Published 20 April 2020 Volume 2020:13 Pages 1111—1121


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Professor Suresh Antony

Pourya Gholizadeh,1,2 Şükran Köse,3 Sounkalo Dao,4 Khudaverdi Ganbarov,5 Asghar Tanomand,6 Tuba Dal,7 Mohammad Aghazadeh,8 Reza Ghotaslou,8 Mohammad Ahangarzadeh Rezaee,8 Bahman Yousefi,8 Hossein Samadi Kafil2,9

1Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; 2Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 3Department of Infectious Diseases and Clinical Microbiology, University of Health Sciences, Tepecik Training and Research Hospital, İzmir, Turkey; 4Faculté de Médecine, de Pharmacie et d’Odonto-Stomatologie (FMPOS), University of Bamako, Bamako, Mali; 5Department of Microbiology, Baku State University, Baku, Republic of Azerbaijan; 6Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran; 7Department of Clinical Microbiology, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara, Turkey; 8Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 9Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Correspondence: Hossein Samadi Kafil
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Tel +98 9127184735
Fax +98 4133364661

Abstract: Rapid emergence of antibiotic-resistant bacteria has made it harder for us to combat infectious diseases and to develop new antibiotics. The clustered regularly interspaced short palindromic repeats – CRISPR-associated (CRISPR-Cas) system, as a bacterial adaptive immune system, is recognized as one of the new strategies for controlling antibiotic-resistant strains. The programmable Cas nuclease of this system used against bacterial genomic sequences could be lethal or could help reduce resistance of bacteria to antibiotics. Therefore, this study aims to review using the CRISPR-Cas system to promote sensitizing bacteria to antibiotics. We envision that CRISPR-Cas approaches may open novel ways for the development of smart antibiotics, which could eliminate multidrug-resistant (MDR) pathogens and differentiate between beneficial and pathogenic microorganisms. These systems can be exploited to quantitatively and selectively eliminate individual bacterial strains based on a sequence-specific manner, creating opportunities in the treatment of MDR infections, the study of microbial consortia, and the control of industrial fermentation.

Keywords: antibiotic-resistant bacteria, CRISPR-Cas system, sequence-specific manner, resensitization, genome editing

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