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HCP5 Promotes Proliferation and Contributes to Cisplatin Resistance in Gastric Cancer Through miR-519d/HMGA1 Axis

Authors Zhang Z, Wang H

Received 2 November 2020

Accepted for publication 31 December 2020

Published 27 January 2021 Volume 2021:13 Pages 787—794

DOI https://doi.org/10.2147/CMAR.S289997

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 5

Editor who approved publication: Dr Seema Singh


Zhu Zhang, Huahong Wang

Department of Gastroenterology, Peking University First Hospital, Beijing 100034, People’s Republic of China

Correspondence: Huahong Wang
Department of Gastroenterology, Peking University First Hospital, Beijing 100034, People’s Republic of China
Tel +86-10-83572301
Email Wanghhbjdx08@126.com

Introduction: The long-non-coding RNA HCP5 (HLA complex P5) has been extensively linked to the ability of cancer cells to resist chemotherapeutic interventions. Here, we investigated the role of HCP5 in gastric cancer (GC) which to-date has been poorly characterized. Our results indicated that HCP5 expression was up-regulated in GC cells.
Methods: HCP5, miR-519d, and high mobility group A1 (HMGA1) expression levels in GC cells were measured using quantitative real-time PCR (qRT-PCR) and Western blot analysis. Drug sensitivity and apoptosis of tumor cells were assessed using cell counting kit-8, flow cytometry, and caspase activity assay. Bioinformatics and luciferase reporter assays were employed for analyzing the interactions between HCP5, miR-519d, and HMGA1.
Results: HCP5 knockdown suppressed proliferation and weakened the resistance to cisplatin (DDP) of GC cells. miR-519d was down-regulated in GC cells and sponged by HCP5. HMGA1 was directly inhibited by miR-519d and its expression was up-regulated in GC cells. HCP5 exacerbated the resistance to cisplatin of GC cells in vitro by enhancing HMGA1 expression via sponging miR-519d.
Conclusion: In summary, HCP5 promoted proliferation and contributed to DDP resistance in GC cells through miR-519d/HMGA1 axis.

Keywords: lncRNA HCP5, gastric cancer, cisplatin, miR-519d, HMGA1

Introduction

Gastric cancer (GC) is one of the most prevalent malignancies and the secondary leading cause of cancer-related deaths worldwide.1 According to the latest global cancer statistics, the deaths of GC are up to 782,685 in 2018, accounting for 8.2% among all cancer deaths in that year.2 Surgical resection is the first choice of treatment for patients with early-stage GC. However, a large proportion of the patients are diagnosed at an advanced stage, and a combination of surgical resection, radiotherapy, and diverse chemotherapy are frequently used for patients with advanced-stage GC.3,4 Due to its high recurrence rate and high metastasis rate, the overall survival rate of patients with GC still remains poor.5 Hence, safe and effective drugs capable of preventing GC are urgently required to improve the therapeutic outcomes.

RNAs that lack coding function (non-coding) regulate gene expression in a range of cell types. Specifically, these RNAs that exceed 200 nts in length are termed lncRNAs, the dysregulation of which have been implicated in tumor growth and formation.6,7 In various cancers, lncRNAs can promote cancer formation or can act as inhibitors of metastatic processes. Many lncRNAs contribute to tumor prognosis, cancer diagnosis,8 and drug resistance,9 such as sunitinib resistance in renal cancer10 and DDP resistance in GC.11 LncRNA HLA complex P5 (HCP5) is primarily found expressed in immune system cells. Recently, lncRNA HCP5 has been reported to promote the growth, metastasis, and drug resistance in various cancers, such as breast cancer,12 pancreatic cancer,13 and lung cancer.14 Nevertheless, the function and mechanism of HCP5 in GC tumorigenesis are still elusive.

MicroRNAs (miRNAs), also belonging to non-coding RNAs (ncRNAs), emerge as a type of endogenous ncRNAs with 19–24 nucleotides, and can bind to the 3ʹ untranslated region (3ʹ-UTR) of target gene mRNA to further modulate the target gene expression.15,16 In addition, miRNAs were reported to actively take part in the regulation of various cellular processes, including tumorigenesis and chemoresistance.17 Previous studies report that miR-519d acted as a tumor suppressor in GC18 and overcame DDP resistance in ovarian cancer cells.19 Furthermore, high mobility group A1 (HMGA1) has been reported to promote proliferation and contribute to DDP resistance in various tumors, such as ovarian cancer and bladder cancer.20,21 The bioinformatics analysis predicted HCP5 and HMGA1 could be targeted via miR-519d. Therefore, we hypothesized that HCP5 might participate in GC progression via modulating miR-519d and HMGA1.

In this work, we investigated the functional role of HCP5 in tumor progression and DDP resistance in GC cells. Additionally, we explored the potential competing endogenous RNA (ceRNA) mechanism of HCP5/miR-519d/HMGA1.

Materials and Methods

Cell Culture and Transfection

Normal gastric cell line (GES-1) and GC cell lines (NCI-N87, MKN-45, SNU-1, and KATO-3) from Shanghai Cell Bank (Shanghai, China) were cultured in Dulbecco’s Modified Eagle Medium/Ham’s Nutrient mixture F12 (DMEM/F12, Gibco, Grand Island, NY, USA) containing 10% FBS (HyClone, Logan, UT, USA) in 5% CO2 at 37°C.

siRNA targeting HCP5 (si-HCP5#1, si-HCP5#2, si-HCP5 #3), si-control (si-con), miR-519d, miR-con, and anti-miR-519d were acquired from GenePharma (Shanghai, China). The sequence of HCP5 was amplified and inserted into the pcDNA-3.1 vector (Invitrogen, Carlsbad, CA, USA) to create pcDNA-HCP5 (oe-HCP5). Cell transfection was conducted by use of Lipofectamine 2000 (Invitrogen), in the light of the manufacturer’s recommendations.

Quantitative Real-Time PCR (qRT-PCR)

Total RNA extraction was conducted following the kit instructions, and Nanodrop-spectrophotometer was employed to measure the RNA purity and concentration. Then, based on the manufacturer’s protocol, complementary DNA (cDNA) was synthesized from 1 µg of total RNA with the PrimeScript-RT Kit (Madison, WI, USA), and then the SYBR® Premix-Ex-Taq™ (Takara, TX, USA) and ABI7300 system were used to perform PCR. All fluorescence data were converted into relative quantification, with U6 and GAPDH treated as the internal reference for miRNA, and mRNA, respectively. The 2−ΔΔCt method was used to analyze the data.

Cell Proliferation and Drug Sensitivity Assay

Cell counting kit-8 (CCK-8) assay was performed to test the rate of cell proliferation. In brief, transfected SNU-1 or KATO-3 cells were plated in the 96-well plates at a density of 1 × 103 cells/well and cultured in an incubator at indicated time. Following that, cell viability was determined by using Cell Counting Kit-8 (Keygen, China).

The GC cells were planted into 96-well plates, followed by incubation with different concentrations (0.5, 1, 2, 4, 8, 16, 32 μM) of DDP. At 48 h after cultivation, the cells were incubated with CCK-8 solution (Beyotime, Shanghai, China) for 2 h and then tested for the absorbance at 450 nm using a microplate reader.

EdU Proliferation Assay

Five-ethynyl-2ʹ-deoxyuridine (EdU) incorporation assays were carried out as described previously.22 Briefly, 5 x 103 cells per well were grown in a 96-well dish and processed with the EdU labeling kit (RiboBio, Guangzhou, China).

Flow Cytometric Analysis

Annexin V-FITC/PI Apoptosis Detection Kit was bought from KeyGEN Biotech (Nanjing, China). Briefly, cells harvested during logarithmic growth were suspended in Annexin-binding buffer. Then, the cells were stained using Annexin V/FITC and PI solution and incubated in the dark at room temperature for 15 min. Finally, cells were detected by flow cytometry analysis.

Caspase Activity Assay

The activities of caspase-3 and caspase-9 in transfected SNU-1 and KATO-3 cells treated with 4 μM DDP were detected using a colorimetric assay kit (R&D Systems Inc., Minneapolis, MN, USA) according to the manufacturer’s instruction.

Luciferase Reporter Assay

The dual-luciferase activity detection kit was purchased from Promega Corporation (Promega, Madison, WI, USA), and luciferase reporter vectors were synthesized by Promega Corporation. Reporter gene plasmids for wild-type (WT) and mutant-type (MUT) HCP5 and HMGA1-3ʹUTR were constructed and co-transfected into GC cells with miR-519d and miR-con, respectively. After 48 h of transfection, for each group, firefly luciferase and renilla luciferase activity were detected with a microplate reader.

Western Blotting

The protein expression levels of HMGA1 were evaluated in SNU-1 and KATO-3 cells as previously described.23 The following antibodies were used: anti-rabbit HMGA1 (Abcam, Cambridge, UK) (1:1000), anti-rabbit GAPDH ((Cell Signaling Technology, Inc., Danvers, MA, USA)) (1:1000), anti-rabbit HRP-linked secondary IgG antibody (Cell Signaling) (1:2000).

Statistical Analysis

Mean ± standard deviation was the expression form of all data. One-way ANOVA was performed for comparing the data difference among 3 or more groups, and t-test was utilized for comparing the difference between two groups. P < 0.05 represented that differences were of statistical significance.

Results

HCP5 Was Overexpressed in GC Cells

To investigate the biological role of lncRNA HCP5 in GC, we analyzed the data of TCGA Stomach Adenocarcinoma (STAD) and found that HCP5 was significantly up-regulated in GC tumor tissues (Figure 1A). To further confirm the level of HCP5 in GC cells, we analyzed HCP5 expression in GC cell lines (NCI-N87, MKN-45, SNU-1, and KATO-3) and normal gastric cell line (GES-1). HCP5 was significantly increased in GC cells compared with GES-1 cells, especially in SNU-1 and KATO-3 cells (Figure 1B). Collectively, HCP5 up-regulation may be implicated with GC tumorigenesis.

Figure 1 HCP5 was increased in GC cells. (A) TCGA dataset analysis of HCP5 expression in GC tumor or normal tissues. (B) qRT-PCR analysis indicated higher levels of HCP5 expression in GC cells or normal cell line GES-1. *P < 0.05, ***P < 0.001.

HCP5 Silencing Inhibited Cell Proliferation and Enhanced DDP Sensitivity in GC Cells

To confirm the functional role of HCP5 in SNU-1 and KATO-3 cells, the cells were transfected with si-con, si-HCP5#1, si-HCP5#2, or si-HCP5#3, and the transfection efficiency was identified using qRT-PCR. The results revealed that the expression of HCP5 was markedly reduced in si-HCP5 group (Figure 2A and B). To investigate the effect of HCP5 on cell proliferation in GC cells, CCK-8 and EdU proliferation assays were performed in SNU-1 and KATO-3 cells transfected with si-con or si-HCP5 #2. As expected, HCP5 knockdown suppressed cell proliferation in SNU-1 and KATO-3 cells (Figure 2CF). To further explore the effect of HCP5 on DDP resistance in GC cells, si-con or si-HCP5 #2 transfected SNU-1 and KATO-3 cells were treated with increasing doses (0.5, 1, 2, 4, 8, 16, 32 μM) of DDP and then subjected to CCK-8 assay. The results indicated that HCP5 silencing could sensitize SNU-1 and KATO-3 cells to DDP, evidenced by the reduced IC50 value (Figure 3A and B). In parallel, knockdown of HCP5 promoted the apoptosis of SNU-1 and KATO-3 cells in the presence of DDP, as determined by flow cytometry analysis and Caspase activity assay (Figure 3CF). Collectively, knockdown of HCP5 suppressed proliferation and improved DDP sensitivity in GC cells.

Figure 2 HCP5 knockdown suppressed cell proliferation of GC cells. (A and B) qRT-PCR analysis was performed in SNU-1 and KATO-3 cells transfected with HCP5 siRNAs (si-HCP5 #1, si-HCP5 #2, si-HCP5 #3) or si-con. (C and D) Cell viability was determined by CCK-8 assays in SNU-1 and KATO-3 cells transfected with si-HCP5 #2 or si-con. (E and F) The proliferation rate of SNU-1 and KATO-3 cells was determined by EdU proliferation assay. *P < 0.05.

Figure 3 HCP5 knockdown improved DDP sensitivity in GC cells. (A and B) Cell viability was determined by CCK-8 assays in si-con or si-HCP5 #2 transfected SNU-1 and KATO-3 cells exposed to different concentrations of DDP (0.5, 1, 2, 4, 8, 16, 32 μM) for 48 h. Cell apoptosis was determined by flow cytometry (C and D) and caspase activity assay (E and F) in si-HCP5 #2 or si-con transfected SNU-1 and KATO-3 cells treated with 4 μM DDP. *P < 0.05.

HCP5 Acts as a Sponge of miR-519d in GC Cells

To investigate the downstream targets of HCP5, we performed the online bioinformatics database starBase 3.0 prediction, which indicated that HCP5 had the binding site for miR-519d (Figure 4A). Dual-luciferase reporter gene assay indicated that miR-519d mimics could inhibit the luciferase activity of HCP5-WT, while it did not significantly affect the luciferase activity of HCP5-MUT (Figure 4B and C). Moreover, qRT-PCR showed that HCP5 overexpression could inhibit miR-519d expression, while si-HCP5 #2 promoted its expression (Figure 4D and E). To sum up, HCP5 directly targeted miR-519d and negatively regulated its expression in GC cells.

Figure 4 HCP5 acted as a sponge of miR-519d in GC cells. (A) Bioinformatics analysis predicted the binding site between HCP5 and miR-519d. (B and C) Dual-luciferase reporter activity experiment was conducted to detect the binding relationship between HCP5 and miR-519d. (D and E) After si-HCP5, oe-HCP5 and oe-HCP5-MUT transfections, qRT-PCR was utilized for detecting miR-519d expression in GC cells. Oe represented overexpression. *P < 0.05.

HCP5 Knockdown Suppresses Proliferation and Facilitates DDP Sensitivity of GC Cells Through Adsorbing miR-519d

The foregoing experiments showed that HCP5 could adsorb miR-519d, thereby inhibiting its expression. To investigate the effect of HCP5 on cell proliferation by adsorbing miR-519d, SNU-1, and KATO-3 cells were transfected with si-HCP5 #2 or si-HCP5 #2+anti-miR-519d. qRT-PCR verified the success of the transfection (Figure 5A). CCK-8 and EdU proliferation assays revealed that miR-519d inhibition weakened the suppressive effect of si-HCP5 #2 on cell proliferation in SNU-1 and KATO-3 cells (Figure 5BD). Subsequently, drug sensitivity, flow cytometry, and caspase activity assays manifested that miR-519d inhibition debilitated the enhancive effect of si-HCP5 #2 on DDP sensitivity and apoptosis (Figure 5EH). These indicated that HCP5 silencing could inhibit GC proliferation and enhance DDP sensitivity by regulating miR-519d expression.

Figure 5 HCP5 silencing inhibited proliferation and facilitated DDP sensitivity in GC cells through adsorbing miR-519d. SNU-1 and KATO-3 cells were transfected with si-con, si-HCP5 #2 or si-HCP5 #2 + anti-miR-519d. (A) qRT-PCR was utilized for detecting the expression of miR-519d after the co-transfection. CCK-8 assay (B and C) and EdU proliferation assay (D) were performed for detecting cell proliferation after the co-transfection. (E) Drug sensitivity assay was carried out to measure IC50 of DDP after the co-transfection. Flow cytometry analysis (F) and caspase activity assay (G and H) were performed for detecting apoptosis of GC cells after co-transfection. * P < 0.05.

HCP5 Promotes HMGA1 Expression in GC Cells Through Sponging miR-519d

Public database starBase 3.0 prediction shows the binding site between miR-519d and HMGA1 (Figure 6A). Consistently, dual-luciferase reporter gene assay suggested that the luciferase activity of HMGA1-WT construct was significantly decreased after miR-519d transfection, which was blocked by up-regulation of HCP5. However, there was no significant change in the luciferase activity of HMGA1-MUT in all groups (Figure 6B and C). miR-519d mimics or HCP5 silencing could suppress HMGA1 expression, while miR-519d inhibitors reversed the inhibitory effect of si-HCP5 #2 on HMGA1 expression (Figure 6D and E). All the above-mentioned findings supported that HCP5 elevated HMGA1 expression in GC cells through adsorbing miR-519d.

Figure 6 HCP5 elevated HMGA1 expression in GC cells through sponging miR-519d. (A) Bioinformatics analysis predicted the binding sequence of HMGA1 to miR-519d. (B and C) Dual-luciferase activity experiment was conducted to detect the binding relationship between HMGA1 and miR-519d. (D and E) The effect of miR-519d, si-HCP5 #2, or si-HCP5 #2 + anti-miR-519d on HMGA1 mRNA and protein expression level. *P < 0.05.

Discussion

During the past several decades, in spite of the remarkable progresses in diagnostic and therapeutic methods of GC, the long-term survival of GC patients is unsatisfactory because of cancer recurrence and chemoresistance. Thus, deeply investigating key signaling pathway driving GC progression and chemoresistance is crucial for discovering new therapeutic targets and strategies of GC.

Recent evidences have shown that lncRNA participates in multistep of cancer development by affecting key genes modulating cell proliferation, apoptosis, and invasion. HCP5, a novel identified lncRNA, is overexpressed in many cancers.12–14 Moreover, emerging evidence suggested that HCP5 was implicated in chemoresistance.13,24 Particularly, HCP5 contributed to DDP resistance in breast cancer cells through inhibiting phosphatase and tensin homolog (PTEN) expression.25 Nevertheless, until now, the HCP5 function in GC is still unclear. In this research, real-time PCR analysis results showed that GC cells exhibited higher HCP5 levels than controls. Additionally, HCP5 knockdown suppressed cell proliferation and DPP resistance in GC cells, suggesting the tight relationship between HCP5 and GC development and chemoresistance.

Mounting researches proposed that the lncRNAs could adsorb miRNA to set free miRNA’s target genes, thus participating in multiple pathogenic processes especially in cancer. Herein, Dual-luciferase assay results verified that miR-519d could bind to HCP5 directly. HCP5 knockdown increased the miR-519d level, while miR-519d overexpression had no influences on HCP5 expression. As expected, GC cells displayed lower miR-519d levels than controls. Functional results revealed HCP5 knockdown suppressed cell proliferation and overcame DDP resistance in GC cells, while miR-519d inhibitor could offset these effects caused by HCP5 ablation, suggesting HCP5 modulated GC development and chemoresistance in a miR-519d dependent manner. Moreover, HMGA1 was further verified as a miR-519d target in this work. HMGA1 is an oncogene, which is required for various types of tumor development by modulating cancer cell apoptosis, growth, and invasion.26–29 In addition, HMGA1 has been reported to confer DDP resistance in many tumors, including ovarian cancer and bladder cancer.20,21 In this work, consistently, we proved that miR-519d could suppress the expression of HMGA1 via targeting it, and HCP5 could positively regulate HMGA1, which could be reversed by miR-519d. Therefore, HCP5 could elevate HMGA1 expression through sponging miR-519d in GC. These demonstrations partly explained the mechanism by which HCP5 participated in GC progression and DDP chemoresistance.

In summary, our findings disclose an unexplored HCP5/miR-519d/HMGA1 axis in regulating gastric cancer progression and DDP resistance. Therefore, it may provide a promising therapeutic option for gastric cancer treatment.

Disclosure

No competing financial interests exist. The authors report no conflicts of interest for this work.

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