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Genistein treatment increases bone mass in obese, hyperglycemic mice

Authors Michelin R, Al-Nakash L, Broderick T, Plochocki J

Received 3 October 2015

Accepted for publication 6 January 2016

Published 15 March 2016 Volume 2016:9 Pages 63—70

DOI https://doi.org/10.2147/DMSO.S97600

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Pietro Scicchitano

Peer reviewer comments 3

Editor who approved publication: Professor Ming-Hui Zou


Richard M Michelin,1 Layla Al-Nakkash,2 Tom L Broderick,3 Jeffrey H Plochocki4

1Arizona College of Osteopathic Medicine, 2Department of Physiology, 3Laboratory of Diabetes and Exercise Metabolism, Department of Physiology, 4Department of Anatomy, Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ, USA

Background:
Obesity and type 2 diabetes mellitus are associated with elevated risk of limb bone fracture. Incidences of these conditions are on the rise worldwide. Genistein, a phytoestrogen, has been shown by several studies to demonstrate bone-protective properties and may improve bone health in obese type 2 diabetics.
Methods: In this study, we test the effects of genistein treatment on limb bone and growth plate cartilage histomorphometry in obese, hyperglycemic ob/ob mice. Six-week-old ob/ob mice were divided into control and genistein-treated groups. Genistein-treated mice were fed a diet containing 600 mg genistein/kg for a period of 4 weeks. Cross-sectional geometric and histomorphometric analyses were conducted on tibias.
Results: Genistein-treated mice remained obese and hyperglycemic. However, histomorphometric comparisons show that genistein-treated mice have greater tibial midshaft diameters and ratios of cortical bone to total tissue area than the controls. Genistein-treated mice also exhibit decreased growth plate thickness of the proximal tibia.
Conclusion: Our results indicate that genistein treatment affects bone of the tibial midshaft in the ob/ob mouse, independent of improvements in the hyperglycemic state and body weight.

Keywords:
obesity, hyperglycemia, genistein, ob/ob mice, bone

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