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Functional connectivity of paired default mode network subregions in primary insomnia

Authors Nie X, Shao Y , Liu S, Li H , Wan A, Nie S, Peng D, Dai X

Received 27 August 2015

Accepted for publication 13 October 2015

Published 16 December 2015 Volume 2015:11 Pages 3085—3093


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Professor Wai Kwong Tang

Xiao Nie,1,* Yi Shao,2,* Si-yu Liu,3 Hai-jun Li,1 Ai-lan Wan,4 Si Nie,1 De-chang Peng,1 Xi-jian Dai1,5

1Department of Radiology, The First Affiliated Hospital of Nanchang University, Nangchang, Jiangxi, People’s Republic of China; 2Department of Ophthalmology,The First Affiliated Hospital of Nanchang University, Nangchang, Jiangxi, People’s Republic of China; 3Medical College of Nanchang University, Nangchang, Jiangxi, People’s Republic of China; 4Department of Psychosomatic Medicine, The First Affiliated Hospital of Nanchang University, Nangchang, Jiangxi, People’s Republic of China; 5Brain Cognition and Brain Disease Institute, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, People’s Republic of China

*These authors contributed equally to this work

Objective: The aim of this study is to explore the resting-state functional connectivity (FC) differences between the paired default mode network (DMN) subregions in patients with primary insomnia (PIs).
Methods: Forty-two PIs and forty-two age- and sex-matched good sleepers (GSs) were recruited. All subjects underwent the resting-state functional magnetic resonance imaging scans. The seed-based region-to-region FC method was used to evaluate the abnormal connectivity within the DMN subregions between the PIs and the GSs. Pearson correlation analysis was used to investigate the relationships between the abnormal FC strength within the paired DMN subregions and the clinical features in PIs.
Results: Compared with the GSs, the PIs showed higher Pittsburgh Sleep Quality Index score, Hamilton Anxiety Rating Scale score, Hamilton Depression Rating Scale score, Self-Rating Depression Scale score, Self Rating Anxiety Scale score, Self-Rating Scale of Sleep score, and Profile of Mood States score (P<0.001). Compared with the GSs, the PIs showed significant decreased region-to-region FC between the medial prefrontal cortex and the right medial temporal lobe (t=-2.275, P=0.026), and between the left medial temporal lobe and the left inferior parietal cortices (t=-3.32, P=0.001). The abnormal FC strengths between the DMN subregions did not correlate with the clinical features.
Conclusion: PIs showed disrupted FC within the DMN subregions.

Keywords: insomnia, functional connectivity, default mode network, functional magnetic resonance imaging, resting-state, blood oxygenation level dependent


Primary insomnia (PI), one of the most prevalent health complaints worldwide, characterized by difficulties in initiating or maintaining sleep, or non-restorative sleep in the absence of any related medical or psychiatric condition, has been associated with cognitive deficits, including the attention, memory, decision making, and executive function.1,2 In spite of a recent increase in the neuroimaging research into the PI, it has yet to establish a consistent conclusion about its neuropathology,3 especially the structural studies of the brain volume alterations.410 On the functional imaging aspects, the studies are too few and diverse in methodology to yield any general conclusions. Altena et al concluded that patients with PI (PIs) are cognitively compromised, as shown by hypoactivation in the left prefrontal cortex and left inferior frontal gyrus during task performance.11 Drummond et al12 further investigated the cognitive impairments in the PIs, they found less activation in the thalamus, fronto-parietal cortex, and cerebellum, and these brain regions normally associated with the working memory and the motor and visual processing during the cognitive tasks compared with the good sleepers (GSs).12 Previous study found that the aberrant activation of the insula, which integrates temporal and bodily states, in arousal networks may underlie the misperception of sleep quality and subjective distress in insomnia.13 Our previous study found that both female and male PIs showed different regional homogeneity in the temporal cortex, cingulate cortex, cerebellum, and frontal gyrus.2 Although these studies provided insights into the neural events occurring in the PIs, there was far less evidence for the neuromechanism changes.

It is proposed that the resting-state functional magnetic resonance imaging (rs-fMRI), one of the hot areas in neuroimaging and one that is suitable for the mechanism research of central nervous system, can detect the spontaneous neuronal activity of the human brain and provide new insights into the pathophysiology of disease, because of its advantages in not requiring exposure to radioactive tracers, accurate positioning, and ease of combining functional imaging with structural imaging. Functional connectivity (FC) that is an important part of the rs-fMRI has been widely used as a technique for unbiased analysis of the brain’s functional connectome.14,15 Previous studies demonstrated that the PIs relative to the GSs showed reduced FC between the left orbitofrontal cortex and the left caudate head,16 and between the parietal lobe and the frontal lobe.17 Huang et al found decreased FC mainly between the amygdala and the insula, striatum, and thalamus, and increased FC mainly between the amygdala and the premotor cortex and sensorimotor cortex in the PIs compared with the GSs.18

The “default-mode network” (DMN) is an organized functional network of several brain regions. Anatomically, the DMN spans the bilateral inferior parietal cortices (IPC), posterior cingulate cortex, precuneus, medial prefrontal cortex (MPFC), retrosplenial cortex, and parts of the hippocampal formation and medial temporal lobe (MTL).19,20 Recent researches have suggested that the DMN might be associated with the collection and evaluation of information,21 self-referential mental activity,22 extraction of episodic memory,23 emotion and anxiety,24,25 mind wandering or daydreaming,26 and autobiographical memory retrieval and envisioning the future when individuals are not focusing on the external environment.2729 More and more researches have been devoted to exploring the DMN changes in various kinds of diseases, including the sleep deprivation,30,31 cognitive impairment,32 and autism.33 However, far less is still known about the effect of PIs on the intrinsic DMN node connectivity. In this study, we examined the FC changes of the paired DMN subregions in the PIs using the seed-based region-to-region FC method to explore its possible neural mechanisms.

Materials and methods

Forty-two PIs (15 males and 27 females; mean age: 49.24±12.26) who had sleep onset and/or maintenance insomnia were recruited from the Psychiatry Department of our Hospital, and forty-two GSs (18 males and 24 females; mean age: 49.14±10.20) who were age, sex, and education status-matched to the PIs were recruited from the community via a newspaper advertisement. Twenty-three PIs (seven males and 16 females) were not the first-time visitors and had taken hypnotic medications or psychoactive medications before, the other nineteen PIs (eight males and eleven females) were first-time visitors and had never taken medications before. The medication history duration was from 1 month to 5 years. Before the tests, the PIs were asked to stop taking any medications for at least 2 weeks before the data collection and for the duration of the study; however, three PIs only stopped taking agents 2–4 days before the test.

The PIs met the following criteria as in our previous study where the regional homogeneity results of a group of 24 PIs from the 42 PIs in our study were reported:2 conformity to the pertinent diagnostic criteria as defined by the International Classification of Sleep Disorder-2,34 higher duration of insomnia than 2 months, higher score of Pittsburgh Sleep Quality Index (PSQI) than 5, a sleep diary for at least 2 weeks’ duration, and right-handedness. All GSs met the following criteria: a good sleeping habit and good sleep onset and/or maintenance, a regular dietary habit, no consumption of any stimulants, medications, tea or coffee for at least 3 months before the study, lower score of PSQI than five, and lower score of Hamilton Depression Rating Scale (HAMD) and Hamilton Anxiety Rating Scale (HAMA) scores <7.

The exclusion criteria for both groups comprised pathological brain MRI findings, inborn or other acquired diseases, any foreign implants in the body, present, or past psychiatric or central nervous system disorders, substance dependency or substance abuse (including heroin, nicotine, or alcohol addiction for GSs), foreign implants in the body, any history of swing shift, shift work, sleep complaints or other sleep disorder, including the hypersomnia, parasomnia, sleep-related breathing disorder, sleep-related movement disorder, or circadian rhythm sleep disorder.

Research design and procedures

An experienced psychiatrist evaluated the PIs with the Diagnostic and Statistical Manual of Mental Disorders, version 4 (DSM-IV)35 for the life history of psychiatric disorders, as well as an unstructured clinical interview for the history of medical and sleep disorders. To evaluate the sleep status, the PIs were asked to wear a Fitbit Flex tracker ( for two consecutive nights and the GSs for 1 week. During the time, the total sleep time, sleep onset latency, sleep efficiency, and number of awakenings were recorded.

The volunteers were instructed to wear black blinders and sponge earplugs, and fix the head, to avoid audiovisual stimulus during the rs-fMRI scans. They were told to relax and not to think of anything, and not to fall asleep in particular. A simple questionnaire was administered immediately after the scans to determine whether the subjects were awake during the session. The data of the subjects who were asleep during the scans were excluded. This study was approved by The Human Research Ethics Committee of our Hospital. All volunteers participated voluntarily and were informed of the purposes, methods, and the potential risks, and all signed an informed consent form.


All volunteers were asked to complete a number of questionnaires, including the PSQI,36 Insomnia Severity Index,37 HAMD,38 HAMA,39 Self-Rating Depression Scale,40 Self Rating Anxiety Scale,41 Self-Rating Scale of Sleep, and Profile of Mood States.42,43

MRI parameters

MRI scanning was performed on a 3-Tesla MR scanner (Trio, Siemens, Erlangen, Germany). High-resolution T1-weighted images were acquired with a three-dimensional spoiled gradient-recalled sequence in an sagittal orientation: 176 images (repetition time =1,900 ms, echo time =2.26 ms, thickness =1.0 mm, gap =0.5 mm, acquisition matrix =256×256, field of view =250 mm ×250 mm, flip angle =9°) were obtained. Finally, an 8-minute rs-fMRI scan was obtained with eyes closed. A total of 240 functional images (repetition time =2,000 ms, echo time =30 ms, thickness =4.0 mm, gap =1.2 mm, acquisition matrix =64×64, flip angle =90°, field of view =220 mm ×220 mm, 29 axial slices with Gradient-Recalled Echo-Planar Imaging pulse sequence) covering the whole brain were obtained.

Data preprocessing

Functional data were checked by MRIcro software ( to exclude the defective data. The first ten time points of the functional images were discarded due to the possible instability of the initial MRI signal and the participants’ adaptation to the scanning environment. On the basis of MATLAB2010a (Mathworks, Natick, MA, USA), the rest of the data pre-processing was performed by the DPARSFA ( software, including digital imaging and communications in medicine form transformation, slice timing, head-motion correction, spatial normalization, and smooth with a Gaussian kernel of 6×6×6 mm3 full-width at half-maximum. The participants who had more than 1.5 mm maximum translation in x, y, or z and 1.5° of motion rotation were rejected. The Friston six head-motion parameters were used to regress out head-motion effects based on recent work, showing that higher-order models were more effective in removing head-motion effects.44,45 Linear regression was also applied to remove other sources of spurious covariates along with their temporal derivatives, including the signal from a ventricular regions of interest, and the signal from a region centered in white matter.27 Of note, the global signal was not regressed out in the present data, as in our previous studies,2,46,47 for the reason that there is still a controversy concerning removing the global signal in the preprocessing of resting-state data.27,48 After the head-motion correction, the align fMRI images were spatially normalized to the Montreal Neurological Institute space and re-sampled at a resolution of 3 mm ×3 mm ×3 mm. After the pre-processing, the time series for each voxel was linearly detrended and filtered (bandpass 0.01–0.08 Hz) to reduce the low-frequency drift, high-frequency physiological respiratory and cardiac noise.49

Definition of DMN seed regions

According to the previous studies,5052 we defined the eight canonical core regions within the DMN: posterior cingulate cortex, MPFC, bilateral hippocampal formation, bilateral MTL, and bilateral IPC (Table 1, Figure 1). The average time courses of these eight regions were defined by placing spherical seeds (r=6 mm) and then extracted from each subject.

Table 1 The coordinate of the definition of the DMN subregions
Note: The coordinate of the eight canonical core regions within the DMN.
Abbreviations: DMN, default mode network; MNI, Montreal Neurological Institute; R, right; L, left.

Figure 1 Regions of interest within the DMN.
Notes: The red spots in the axial slices represent the DMN subregions (radius =6 mm). The z-value represents the Z direction of the MNI coordinate.
Abbreviations: PCC, posterior cingulate cortex; MPFC, medial prefrontal cortex; HF, hippocampus formation; IPC, inferior parietal cortices; MTL, medial temporal lobe; DMN, default mode network; MNI, Montreal Neurological Institute.

FC analysis

For each subject, a correlation map was produced by computing the correlation coefficient (r score) between each pair of the DMN regions, totally yielding the 28 paired connectivity. Correlation coefficients were then converted to z-values using Fisher’s r-to-z transform to standardize the statistic analysis because the correlation coefficient r is not normally distributed.

Correlation analysis

To investigate the relationships between the clinical measures and the FC strength of the region-to-region within the DMN in PIs, the z-values of the temporal correlation coefficients of the different paired connectivity between the PIs and the GSs were correlated with the clinical questionnaires using the Pearson correlation analysis. The threshold was set at a significance level of P<0.05.

Statistical analysis

For the clinical measures, two-sample Student’s t-test (homoscedasticity) and Mann–Whitney U-test (heteroscedasticity) were used for the continuous data. All the results are quoted as two-tailed P-values. P<0.05 was considered as statistically significant. All the statistical analyses were performed using IBM SPSS version 21.0 statistical software.

Within each group, a random-effect one-sample t-test was performed on the individual z-value maps of the 28 pairs of DMN subregions. A corrected significance level of P<0.05, using an false-positive adjustment, was performed for the multiple comparison corrections.53,54 The two-sample t-test was performed on the individual z-value maps of the 28 pairs of DMN subregions to determine the FC differences between the PIs and the GSs with the age, sex, and education as covariates. A corrected significance level of individual voxel P<0.05, using the false-positive adjustment, was used to determine the statistical significance.


Demographic and clinical questionnaires

Demographic and clinical questionnaires of each group are summarized in Table 2. No significant differences were found in age (t=−0.996; P=0.322), sex (χ2=0.645; P=0.422), and education (t=−0.408; P=0.684) between the PIs and the GSs (P>0.05). Compared with the GSs, the PIs showed higher PSQI score (P<0.001), shorter total sleep time (P<0.001) and lower sleep efficiency (P<0.001), had worse subjective sleep estimate as measured by the Self-Rating Scale of Sleep (P<0.001), and demonstrated disturbed mood state as measured by the HAMA (P<0.001), HAMD (P<0.001), Self Rating Anxiety Scale (P<0.001), Self-Rating Depression Scale (P<0.001), and Profile of Mood States (P<0.001). The Fitbit Flex tracker found that the no significant difference in the total sleep time (P=0.862) between the PIs and the GSs, but the PIs showed lower sleep efficiency (P<0.001), longer sleep onset latency (P<0.001), and more number of awakenings (P<0.001) compared with the GSs.

Table 2 Demographics and characteristics of the PIs and GSs
Notes: Compared with the GSs, the PIs showed significant differences in the sleep characteristics and Fitbit Flex tracker data (P<0.001). No significant differences were found in age, sex, and education between the PIs and the GSs (P>0.05).
Abbreviations: PIs, patients with primary insomnia; GSs, good sleepers; N/A, not applicable; PSQI, Pittsburgh Sleep Quality Index; SRSS, Self-Rating Scale of Sleep; ISI, Insomnia Severity Index; HAMA, Hamilton Anxiety Rating Scale; HAMD, Hamilton Depression Rating Scale; SAS, Self Rating Anxiety Scale; SDS, Self-Rating Depression Scale; POMS, Profile of Mood States.

FC results

Figure 2 shows the FC results of the 28 pairs of DMN subregions in the PIs and the GSs, respectively. In each group, all these DMN regions were strongly connected to each other. Compared with the GSs, the PIs demonstrated two significant decreased FC regions, that is, between the MPFC and the right MTL (MTL.R) (t=−2.275, P=0.026), and between the left MTL (MTL.L) and the left IPC (IPC.L) (t=−3.32, P=0.001) (Figure 3). No other region-to-region connectivities were found.

Figure 2 Correlation matrix of average time series of pair-wise subregions in DMN.
Note: The pictures represent the FC results of the 28 pairs of DMN subregions in PIs (A) and GSs (B), respectively by one-sample t-test.
Abbreviations: PIs, patients with primary insomnia; GSs, good sleepers; DMN, default mode network; FC, functional connectivity; PCC, posterior cingulate cortex; MPFC, medial prefrontal cortex; HF, hippocampus formation; IPC, inferior parietal cortices; MTL, medial temporal lobe; R, right; L, left.

Figure 3 FC differences within the DMN between the PIs and the GSs.
Notes: Comparison of FC within the DMN in the PIs versus the GSs, The PIs showed decreased FC between the MPFC and the MTL.R, and between the MTL.L and the IPC.L compared with the GSs. Undirected edges correspond to the significance of differences (t-values).
Abbreviations: FC, functional connectivity; DMN, default mode network; PIs, patients with primary insomnia; GSs, good sleepers; PCC, posterior cingulate cortex; MPFC, medial prefrontal cortex; HF, hippocampus formation; IPC, inferior parietal cortices; MTL, medial temporal lobe; R, right; L, left.

No correlation results

There were no significant correlations between the region-to-region FC strength within the DMN and the clinical questionnaires (P>0.05).


To the best of our knowledge, this study was the first to use the region-to-region FC method to investigate the FC differences of the DMN subregions, so as to better understand the underlying neural mechanisms of the PIs. Fortunately, in the present study we found that the PIs showed decreased FC between the MPFC and the right MTL, and between the left MTL and the left IPC compared with the GSs. Although no significant correlations were found between the abnormal paired FC subregions and the clinical measurements, the PIs showed more negative emotions compared with the GSs.

Recent researches indicate that the prefrontal cortex is particularly sensitive to the sleep, and has a major role in mediating sleep physiology.5557 Several neuropsychological studies have revealed that the PIs had impaired performance in tests involving the prefrontal cortex.58,59 The MPFC, as a core region of the DMN, associated with the social cognitive processes, is related to decision making and self-regulations.60,61 Koenigs et al found that the focal injuries to the left DMPFC are associated with the sleep initiation and maintenance.62 Joo et al found that the PIs showed significant decreased gray matter volume in the MPFC compared with the GSs.10 Our previous study found that male PIs showed lower regional homogeneity in the MPFC compared with male GSs.2 These studies demonstrated that the PIs had a disturbed function and structure in the MPFC.

The MTL, which consists of several critical memory-related structures, including the hippocampus, amygdala, cingulate gyrus, and the surrounding hippocampal areas (such as the entorhinal, perirhinal, and parahippocampal cortices),63 was associated with a variety of sensory information integration. The MTL is considered as a sensitive predictor for conversion to Alzheimer’s disease.6466 Nofzinger et al reported that the PIs have a smaller reduction in relative metabolism from wakefulness to non-rapid eye movement sleep in the hippocampus, parahippocampal, and amygdaloid.67 Our previous study found that the PIs showed lower regional homogeneity area in the MTL.2 These studies demonstrated that the PIs had a functional disorder in the MTL. Furthermore, previous studies showed that the MTL (entorhinal cortex, parahippocampal cortex, and perirhinal cortex), connected via the medial dorsal nucleus of the thalamus to the medial, orbital, and lateral prefrontal cortex, which is essential to the declarative memory.6870 Therefore, we speculated that the decreased FC between the MPFC and the right MTL in our study may be associated with the cognitive deficits in PIs.

The IPC, a large and heterogeneous region, is obligatorily or unintentionally engaged in the recall, consolidation, and retrieval of episodic memory information,7173 as well as being implicated in diverse cognitive operations74 that include bodily awareness,75 generating a sense of personal responsibility,76 and moral decision making,77 action, language, and mathematical problem solving.78,79 Chee and Chuah found that the sleep deprivation reduced IPL deactivation during a visual short-term memory task.80 Horovitz et al revealed reductions in the IPC-MPFC FC during deep sleep and after partial sleep deprivation.81,82 De Havas et al also found that the IPC node of the DMN was consistently impaired and might represent an early marker for the effects of 24-hour sleep deprivation, as well as serving as an indicator of hitherto-unexplored behavioral impairments.83 Gao et al found acute sleep deprivation showed decreased amplitude of low frequency fluctuation in the IPC.84 A previous regional homogeneity study found little decreased spontaneous brain activity in the IPC in patients with obstructive sleep apnea.85 Our previous sleep deprivation study found that a total of 72 hours sleep deprivation disturbed the spontaneous activity of the IPC area and its connectivity pattern with other DMN subregions.30 These studies showed consistent evidence that the IPC had abnormal spontaneous activity and connectivity pattern in the sleep disorders.

Recent studies suggest a role of DMN parietal regions during retrieval of information, which in concert with the medial temporal structures.86 Meanwhile, Tessitore et al revealed that the reduced FC between the MTL and the IPC may play a role in the development of cognitive decline in the Parkinson’s disease, and the functional abnormalities precede the structural abnormalities.87 In this study, our findings demonstrated that the PIs compared with the GSs had decreased FC between the MPFC and the right MTL, and between the left MTL and the left IPC. These abnormal connectivities may be a potential functional basis of the emotional, memory, and cognitive decline in the PIs. Although no significant correlations were found between the abnormal paired FC subregions and the clinical measurements, these findings demonstrated the FC impairment of the intrinsic DMN subregions and help us know more about the underlying mechanism of the PIs.


Using the resting-state seed-based FC method, we found decreased region-to-region FC between the MPFC and the right MTL, and between the left MTL and the left IPC. These findings will help us insight into a deeper understanding of the neural mechanism of the PIs. However, there are several limitations that should be paid attention to. First, a larger sample size should be studied. Second, future studies should go beyond examining limited DMN to make a comprehensive analysis. Third, some PIs were selected without reference to the polysomnogram, although all PIs were asked to wear a Fitbit Flex tracker to monitor their sleep quality.


This work was supported by Jiangxi Provincial Department of Science and Technology Support Program (grant No 20132BBG70061, and No 20141BBG70026), Jiangxi Provincial Department of Natural Science Foundation Project (grant No 20132BAB205100), and National Natural Science Foundation of China (grant No 81560285).


The authors report no conflicts of interest in this work.



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