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Examination of the effect of ovarian radiation injury induced by hysterosalpingography on ovarian proliferating cell nuclear antigen and the radioprotective effect of amifostine: an experimental study

Authors Can B, Atilgan R, Pala S, Kuloğlu T, Kiray S, Ilhan N

Received 11 November 2017

Accepted for publication 29 March 2018

Published 25 May 2018 Volume 2018:12 Pages 1491—1500

DOI https://doi.org/10.2147/DDDT.S156757

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Frank M. Boeckler


Behzat Can,1 Remzi Atilgan,1 Sehmus Pala,1 Tuncay Kuloğlu,2 Sule Kiray,3 Nevin Ilhan4

1Department of Obstetrics and Gynecology, Firat University School of Medicine, Elazig, Turkey; 2Department of Histology and Embryology, Firat University School of Medicine, Elazig, Turkey; 3Department of Obstetrics and Gynecology, Maltepe University School of Medicine, Istanbul, Turkey; 4Department of Biochemistry, Firat University School of Medicine, Elazig, Turkey

Aim: The aim of this study was to examine the effect of amifostine on cellular injury in the ovarian tissue induced by hysterosalpingography (HSG).
Methods: In total, forty 4-month old female Wistar Albino rats were assigned into 8 groups. Each group contained 5 rats. Group 1 (G1): rats were decapitated without any procedure. Group 2 (G2): rats were decapitated after 3 hours of total body irradiation. Group 3 (G3): rats were decapitated 3 hours after HSG procedure. Group 4 (G4): rats were decapitated 3 hours after HSG procedure performed 30 min after receiving amifostine 200 mg/kg intraperitoneally. Group 5 (G5): rats were decapitated after 1 month without any procedure. Group 6 (G6): rats were decapitated after 1 month of total body irradiation. Group 7 (G7): rats were decapitated 1 month after HSG procedure. Group 8 (G8): rats were decapitated 1 month after HSG procedure performed 30 min after receiving amifostine 200 mg/kg intraperitoneally. After rats were decapitated under general anesthesia in all groups, blood samples were obtained and bilateral ovaries were removed. One of the ovaries was placed in 10% formaldehyde solution for histological germinal epithelial degeneration, apoptosis and proliferating cell nuclear antigen scoring. The other ovary and blood sera were stored at –80°C. TNF-α, total antioxidant status, total oxidant status, and malondialdehyde levels were studied in tissue samples and anti-mullerian hormone levels in blood samples.
Results: At the end of the first month, there was significant ovarian germinal epithelium degeneration. Proliferating cell nuclear antigen immunoreactivity was significantly reduced in all other groups when compared with G1 and G5.
Conclusion: In conclusion, amifostine could significantly reduce the ovarian cellular injury induced by HSG.

Keywords: hysterosalpingography, amifostine, ovarian damage, radiation

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