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Evaluation of Antihyperglycemic Effect of Extract of Moringa stenopetala (Baker f.) Aqueous Leaves on Alloxan-Induced Diabetic Rats

Authors Woldekidan S, Mulu A, Ergetie W, Teka F, Meressa A, Tadele A, Abebe A, Gemechu W, Gemeda N, Ashebir R, Sileshi M, Tolcha Y

Received 17 September 2020

Accepted for publication 4 December 2020

Published 15 January 2021 Volume 2021:14 Pages 185—192

DOI https://doi.org/10.2147/DMSO.S266794

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 5

Editor who approved publication: Dr Antonio Brunetti


Samuel Woldekidan,1 Abay Mulu,2 Wondwossen Ergetie,3 Frehiwot Teka,1 Asfaw Meressa,1 Ashenif Tadele,1 Abiy Abebe,1 Worku Gemechu,1 Negero Gemeda,1 Rekik Ashebir,1 Meron Sileshi,4 Yoseph Tolcha4

1Directorate of Traditional and Modern Medicine Research, Ethiopian Public Health Institute, Addis Ababa, Ethiopia; 2Department of Anatomy, School of Medicine, College of Health Science, Addis Ababa University, Addis Ababa, Ethiopia; 3Department of Pathology, School of Medicine, College of Health Science, Addis Ababa University, Addis Ababa, Ethiopia; 4National Clinical Chemistry Reference Laboratory, Addis Ababa, Ethiopia

Correspondence: Samuel Woldekidan
Directorate of Traditional and Modern Medicine Research, Ethiopian Public Health Institute, P.O. Box 1242, Addis Ababa, Ethiopia
Email woldekidans6@gmail.com

Background: Diabetes is a serious metabolic disorder with complications that result in significant morbidity and mortality. Current drugs used for diabetes therapy are not free from side effects and do not restore normal glucose homeostasis. Therefore, the purpose of this study is to evaluate the antidiabetic effect of Moringa stenopetala (Baker f.) aqueous leaves extract.
Methods: Thirty rats of weight 90– 150 gram were distributed to five groups (n= 6). Then labelled as diabetic control (DC), normal control (NC), extract treated (MS 250 and 500mg/kg), and glibenclamide treated (GL 5mg/kg). The experimental rats were induced by intra-peritoneal injection of Alloxan monohydrate at a dose of 180 mg/kg after dissolving in normal saline. Clinical biochemistry such as AST, ALT, ALP, urea, creatinine, and cholesterol, blood glucose level, histopathological and preliminary phytochemical screening were evaluated.
Results: Phytochemical tests revealed the presence of different secondary metabolites. Alkaloid, flavonoid, tannin, saponin, phytosteroids, phenols and terpenoids. Moringa stenopetala (Baker f.) leaves aqueous extract (250 and 500mg/kg) improved the body weight of rats, showed remarkable reduction in blood glucose concentration (P< 0.05), and significantly decreased serum urea, creatinine, ALT, AST and ALP (P < 0.05). Levels of serum cholesterol remained unaltered in the experimental groups when compared with diabetic control. Histopathology of non-treated rats showed deterioration of insulin producing pancreas cells; nevertheless, β-cells restoration was observed due to administration of Moringa stenopetala (Baker f.) aqueous leaves extract.
Conclusion: It is possible to conclude that oral administration of Moringa stenopetala (Baker f.) aqueous leaf extracts (250mg/kg and 500mg/kg) for 28 days showed beneficial effects on antihyperglycemia, improved body weight and Alloxan damaged pancreatic β-cells, and restored biochemical changes.

Keywords: diabetes, Moringa stenopetala, pancreatic β-cells, alloxan monohydrate

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