Evaluation of Anti-Biofilm Capability of Cordycepin Against Candida albicans
Authors Wang Y, Pei Z, Lou Z, Wang H
Received 8 October 2020
Accepted for publication 1 December 2020
Published 5 February 2021 Volume 2021:14 Pages 435—448
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Professor Suresh Antony
Yu Wang,1,2,* Zejun Pei,1,2,* Zaixiang Lou,2 Hongxin Wang2
1The Affiliated Wuxi No. 2 People’s Hospital of Nanjing Medical University, Wuxi, 214122, People’s Republic of China; 2School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Zaixiang Lou Tel +86-18751559780
Zejun Pei Tel +86-510-68563496
Introduction: The opportunistic pathogen Candida albicans can form biofilms, resulting in drug resistance with great risk to medical treatment.
Methodology: We investigated the ability of C. albicans to form biofilms on different materials, as well as the inhibitory and eradicating effects of cordycepin on biofilm. The action mechanism of cordycepin against biofilm was studied by crystal violet staining, XTT [2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] reduction method, phenol-sulfuric acid method, cellular superficial hydrophobicity (CSH) assay, and confocal laser scanning microscope observation. We also evaluated the acute toxicity of cordycepin in vivo.
Results: The results showed facile formation of biofilms by C. albicans on polypropylene. The 50% minimum inhibitory concentration (MIC50) of cordycepin was 0.062 mg/mL. A concentration of 0.125 mg/mL significantly decreased biofilm formation, metabolic activity, secretion of extracellular polysaccharides, and relative CSH. Cordycepin could inhibit biofilm formation at low concentration without affecting fungal growth. In addition, cordycepin effectively eradicated 59.14% of mature biofilms of C. albicans at a concentration of 0.5 mg/mL. For acute toxicity, the LD50 (50% of lethal dose) of cordycepin was determined as higher than 500 mg/kg for mice.
Conclusion: The results of this study show that cordycepin significantly inhibited and eradicated biofilms by decreasing metabolic activity, the ratio of living cells, the hydrophobicity, and damaging the extracellular polysaccharides of biofilm. These findings should facilitate more effective application of cordycepin and suggest a new direction for the treatment of fungal infections.
Keywords: cordycepin, Candida albicans, biofilm, inhibition, eradication
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