Back to Journals » International Journal of Nanomedicine » Volume 10 » Issue 1

Electrospun nanofibrous SF/P(LLA-CL) membrane: a potential substratum for endothelial keratoplasty

Authors Chen JZ, Yan CX, Zhu MY, Yao QK, Shao CY, Lu WJ, Wang J, Mo XM, Gu P, Fu Y, Fan XQ

Received 18 November 2014

Accepted for publication 21 February 2015

Published 5 May 2015 Volume 2015:10(1) Pages 3337—3350

DOI https://doi.org/10.2147/IJN.S77706

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Dr Lei Yang

Junzhao Chen,1,* Chenxi Yan,1,* Mengyu Zhu,1,* Qinke Yao,1 Chunyi Shao,1 Wenjuan Lu,1 Jing Wang,2 Xiumei Mo,2 Ping Gu,1 Yao Fu,1 Xianqun Fan1

1Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 2Biomaterials and Tissue Engineering Laboratory, College of Chemistry and Chemical Engineering and Biotechnology, Donghua University, Shanghai, People’s Republic of China

*These authors contributed equally to this work

Background: Cornea transplant technology has progressed markedly in recent decades, allowing surgeons to replace diseased corneal endothelium by a thin lamellar structure. A thin, transparent, biocompatible, tissue-engineered substratum with corneal endothelial cells for endothelial keratoplasty is currently of interest. Electrospinning a nanofibrous structure can simulate the extracellular matrix and have beneficial effects for cell culture. Silk fibroin (SF) has good biocompatibility but poor mechanical properties, while poly(L-lactic acid-co-Ɛ-caprolactone) (P(LLA-CL)) has good mechanical properties but poor biocompatibility. Blending SF with P(LLA-CL) can maintain the advantages of both these materials and overcome their disadvantages. Blended electrospun nanofibrous membranes may be suitable for regeneration of the corneal endothelium. The aim of this study was to produce a tissue-engineered construct suitable for endothelial keratoplasty.
Methods: Five scaffolds containing different SF:P(LLA-CL) blended ratios (100:0, 75:25, 50:50, 25:75, 0:100) were manufactured. A human corneal endothelial (B4G12) cell line was cultured on the membranes. Light transmission, speed of cell adherence, cell viability (live-dead test), cell proliferation (Ki-67, BrdU staining), and cell monolayer formation were detected on membranes with the different blended ratios, and expression of some functional genes was also detected by real-time polymerase chain reaction.
Results: Different blended ratios of scaffolds had different light transmittance properties. The 25:75 blended ratio membrane had the best transmittance among these scaffolds. All electrospun nanofibrous membranes showed improved speed of cell adherence when compared with the control group, especially when the P(LLA-CL) ratio increased. The 25:75 blended ratio membranes also had the highest cell proliferation. B4G12 cells could form a monolayer on all scaffolds, and most functional genes were also stably expressed on all scaffolds. Only two genes showed changes in expression.
Conclusion: All blended ratios of SF:P(LLA-CL) scaffolds were evaluated and showed good biocompatibility for cell adherence and monolayer formation. Among them, the 25:75 blended ratio SF:P(LLA-CL) scaffold had the best transmittance and the highest cell proliferation. These attributes further the potential application of the SF:P(LLA-CL) scaffold for corneal endothelial transplantation.

Keywords: silk fibroin, poly(L-lactic acid-co-Ɛ-caprolactone), B4G12, corneal endothelium, regeneration

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Other articles by this author:

Readers of this article also read:

Efficacy and mechanism of tanshinone IIA liquid nanoparticles in preventing experimental postoperative peritoneal adhesions in vivo and in vitro

Qin F, Ma Y, Li X, Wang X, Wei Y, Hou C, Lin S, Hou L, Wang C

International Journal of Nanomedicine 2015, 10:3699-3717

Published Date: 21 May 2015

Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats

Liu XM, Hu R, Lian HW, Liu Y, Liu J, Liu JW, Lin GM, Liu LW, Duan XJ, Yong KT, Ye L

International Journal of Nanomedicine 2015, 10:3651-3662

Published Date: 20 May 2015

Effects of PVA coated nanoparticles on human immune cells

Strehl C, Gaber T, Maurizi L, Hahne M, Rauch R, Hoff P, Häupl T, Hofmann-Amtenbrink M, Poole AR, Hofmann H, Buttgereit F

International Journal of Nanomedicine 2015, 10:3429-3445

Published Date: 8 May 2015

Formation of curcumin nanoparticles via solution-enhanced dispersion by supercritical CO2

Zhao Z, Xie M, Li Y, Chen A, Li G, Zhang J, Hu H, Wang X, Li S

International Journal of Nanomedicine 2015, 10:3171-3181

Published Date: 29 April 2015

Characterization of nanostructured ureteral stent with gradient degradation in a porcine model

Wang XQ, Shan HL, Wang JX, Hou YC, Ding JX, Chen QH, Guan JJ, Wang CX, Chen XS

International Journal of Nanomedicine 2015, 10:3055-3064

Published Date: 20 April 2015

Pharmacokinetics of quercetin-loaded nanodroplets with ultrasound activation and their use for bioimaging

Chang LW, Hou ML, Hung SH, Lin LC, Tsai TH

International Journal of Nanomedicine 2015, 10:3031-3042

Published Date: 17 April 2015

Synthesis, characterization, and immune efficacy of layered double hydroxide@SiO2 nanoparticles with shell-core structure as a delivery carrier for Newcastle disease virus DNA vaccine

Zhao K, Rong G, Guo C, Luo X, Kang H, Sun Y, Dai C, Wang X, Wang X, Jin Z, Cui S, Sun Q

International Journal of Nanomedicine 2015, 10:2895-2911

Published Date: 13 April 2015

CdTe quantum dots induce activation of human platelets: implications for nanoparticle hemocompatibility

Samuel SP, Santos-Martinez MJ, Medina C, Jain N, Radomski MW, Prina-Mello A, Volkov Y

International Journal of Nanomedicine 2015, 10:2723-2734

Published Date: 2 April 2015

Electrospun gelatin/polycaprolactone nanofibrous membranes combined with a coculture of bone marrow stromal cells and chondrocytes for cartilage engineering

He X, Feng B, Huang C, Wang H, Ge Y, Hu R, Yin M, Xu Z, Wang W, Fu W, Zheng J

International Journal of Nanomedicine 2015, 10:2089-2099

Published Date: 17 March 2015