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Eco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles

Authors Li Z, Ali I, Qiu J, Zhao H, Ma W, Bai A, Wang D, Li J

Received 23 September 2020

Accepted for publication 22 December 2020

Published 19 January 2021 Volume 2021:16 Pages 481—492

DOI https://doi.org/10.2147/IJN.S283677

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Thomas J. Webster


Zhao Li,1,2 Iftikhar Ali,1,3 Jiying Qiu,4 Huanzhu Zhao,1 Wenya Ma,1,2 Aiying Bai,5 Daijie Wang,1 Jingchao Li1

1Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People’s Republic of China; 2College of Life Science, Shandong Normal University, Jinan 250014, People’s Republic of China; 3Department of Chemistry, Karakoram International University, Gilgit 15100, Pakistan; 4Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, People’s Republic of China; 5Jinan Municipal Center for Disease Control and Prevention, Jinan 250001, People’s Republic of China

Correspondence: Daijie Wang; Jingchao Li
Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People’s Republic of China
Tel +86-53182605319
Fax +86-0531-82964889
Email wangdaijie@126.com; lijingchao888@sohu.com

Background: Dihydromyricetin (DMY), a natural flavonoid, has reportedly antibacterial, antioxidant, anticancer and other properties. In the present study, DMY was used as a reducing agent and stabilizer to synthesize silver nanoparticles (AgNPs), and the optimal conditions for its synthesis were studied. The DMY-AgNPs were investigated for their DPPH scavenging properties and their potential against human pathogenic and food-borne bacteria viz. Escherichia coli (E. coli), and Salmonella. In addition, DMY-AgNPs also showed excellent inhibitory effects on cancer Hela, HepG2 and MDA-MB-231 cell lines.
Methods: The dihydromyricetin-mediated AgNPs (DMY-AgNPs) were characterized by ultraviolet-visible spectrophotometer (UV-Vis spectra), scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and X-ray powder diffraction (XRD). Antioxidant activity of DMY-AgNPs was determined by 1.1-diphenyl-2-picrylhydrazyl (DPPH) scavenging. The antibacterial activity was determined by 96-well plate (AGAR) gradient dilution, while anticancer potential was determined by MTT assay.
Results: The results showed that the dispersion of AgNPs had the maximum UV–visible absorption at about 410 nm. The synthesized nanoparticles were almost spherical. FTIR was used to identify functional groups that may lead to the transformation of metal ions into nanoparticles. The results showed that the prepared AgNPs were coated with biological molecules in the extraction solution. The biosynthesized DMY-AgNPs exhibited good antioxidant properties, at various concentrations (0.01– 0.1mg/mL), the free radical scavenging rate was about 56– 92%. Furthermore, DMY-AgNPs possessed good antibacterial properties against Escherichia coli (E. coli), and Salmonella at room temperature. The minimum inhibitory concentrations (MIC) were 10− 6 g/L, and 10− 4 g/L, respectively. The bioactivity of DMY-mediated AgNPs was studied using MTT assay against Hela, HepG2 and MDA-MB-231 cancer cell lines, and all showed good inhibitory effects.
Conclusion: The present study provides a green approach for the synthesis of DMY-AgNPs which exhibited stronger antioxidant, antibacterial and anticancer properties compared to the dihydromyricetin. DMY-AgNPs can serve as an economical, efficient, and effective antimicrobial material for its applications in food and pharmaceutical fields.

Keywords: natural flavonoid, DMY-AgNO3-NPs, green approach, DPPH, Escherichia coli, Salmonella, Hela, HepG2, MDA-MB-231

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