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Dysfunction of microRNA-32 regulates ubiquitin ligase FBXW7 in multiple myeloma disease

Authors Hua J, Ding T, Yang L

Received 6 February 2016

Accepted for publication 1 June 2016

Published 25 October 2016 Volume 2016:9 Pages 6573—6579

DOI https://doi.org/10.2147/OTT.S105945

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Lucy Goodman

Peer reviewer comments 2

Editor who approved publication: Dr William Cho


Jingsheng Hua,1,* Tianling Ding,2,* Linjun Yang3

1Department of Hematology, Taizhou Municipal Hospital, Taizhou, Zhejiang, 2Department of Hematology, Huashan Hospital, Fudan University, Shanghai, 3Department of Surgical Oncology, Taizhou Municipal Hospital, Taizhou, Zhejiang, People’s Republic of China

*These authors contributed equally to this work

Abstract: Dysfunction of microRNA (miRNA) expression has been associated with tumor occurrence, progression, and development. The aim of this work was to study the dysfunction of miR-32 – an miRNA that was abnormally regulated in different tumors – in clinical tissues from patients with multiple myeloma (MM). The tumor tissues in which we assessed miR-32 expression levels were collected during our 5 years of clinical practice. Our study found an increase in miR-32 expression in MM tissues. Assessment of F-box and WD repeat domain-containing 7 (FBXW7) in MM tissues showed an inverse relation between the expression of FBXW7 and miR-32. To further investigate the relation between miR-32 and FBXW7, cells were transfected with miR-32 or anti-miR-32. In vitro studies found that cells transfected with miR-32 showed a lower expression of FBXW7 and a higher expression of cancer-related proteins, c-Jun and c-Myc. In contrast, the cells transfected with anti-miR32 showed a relatively higher expression of FBXW7, but a lower expression of c-Jun and c-Myc. This study may offer perceptive insights into developing new strategies for MM cancer detection and therapy.

Keywords: multiple myeloma, miR-32, F-box and WD repeat domain-containing 7, in vitro
 

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