Downregulation of ribophorin II suppresses tumor growth, migration, and invasion of nasopharyngeal carcinoma
Authors Hong F, Li Y, Ni H, Li J
Received 28 November 2017
Accepted for publication 11 March 2018
Published 15 June 2018 Volume 2018:11 Pages 3485—3494
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 2
Editor who approved publication: Prof. Dr. Geoffrey Pietersz
Feilong Hong, Yong Li, Haifeng Ni, Jing Li
Department of Otolaryngology, Hangzhou First People’s Hospital, Hangzhou, China
Background: It has been reported that ribophorin II (RPN2) expression is increased in many cancers, but the role of RPN2 in nasopharyngeal carcinoma (NPC) remains unclear.
Patients and methods: This study found that the expression of RPN2 is increased dramatically in NPC tissues of patients compared with that in the adjacent normal tissues. This study attempted at understanding the effect of siRNA-RPN2 treatment on the migration and invasion of NPC cell lines CNE2 and HNE1.
Results: RT-PCR and Western blotting showed that RPN2 was highly expressed in CNE2 and HNE1 cells. siRNA-RPN2 treatment significantly inhibited cell viability at 24 and 48 h compared with the control group. Results of the transwell assay showed that, compared to the control groups, migration and invasion of the cells treated with siRNA-RPN2 decreased markedly. In addition, compared to the control groups, caspase-3, caspase-9, and E-cadherin expression levels increased and MMP 2 expression decreased significantly in the siRNA-RPN2-treated group. Phosphorylation of AKT and PI3K was also inhibited after siRNA-RPN2 treatment.
Conclusion: siRNA-RPN2 can effectively inhibit the invasion and migration of human NPC cells via AKT/PI3K signaling. This can serve as a novel strategy for NPC treatment.
Keywords: siRNA-RPN2, nasopharyngeal carcinoma, migration, invasion, MMP2, MMP9
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