Back to Journals » Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy » Volume 13

Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration

Authors Guan CY, Tian S, Cao JL, Wang XQ, Ma X, Xia HF

Received 14 March 2020

Accepted for publication 7 July 2020

Published 24 August 2020 Volume 2020:13 Pages 3009—3034


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Professor Ming-Hui Zou

Chun-Yi Guan,1,2,* Shi Tian,3,* Jing-Li Cao,1,2 Xue-Qin Wang,1,2 Xu Ma,1,2 Hong-Fei Xia1,2

1Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, People’s Republic of China; 2Graduate School, Peking Union Medical College, Beijing Province 100005, People’s Republic of China; 3Haidian Maternal & Child Health Hospital, Beijing 100080, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Xu Ma; Hong-Fei Xia
Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, People’s Republic of China
Tel +86-10-62178932
Fax +86-10-62179059

Purpose: This study aimed to investigate the role of miR-21 expression in the reduction of placental function in GDM patients.
Materials and Methods: qRT-PCR was used to detect the differential expression of miR-21 in the serum of gestational diabetes mellitus (GDM) and normal pregnant women, and to verify the functional target gene PPAR-α of miR-21 by double fluorescence experiments. Cellular experiments were performed to verify the effect of PPAR-α on cell function.
Results: miR-21 is down-regulated in the serum and placenta of GDM patients compared to normal pregnant women. In the case of insulin resistance, miR-21-5p knockdown promoted glucose uptake, but no significant effect was found under physiological condition. Functional studies have shown that reduced PPAR expression can restore miR-21 knockdown-mediated cell growth and metastasis inhibition. Additionally, decreased expression of miR-21 but increased expression of -PPAR-α was observed in patients with GDM and GDM rats.
Conclusion: The expression of the placental miR-21-5p, which inhibits cell growth and infiltration by up-regulating PPAR-α, is downregulated in pregnant GDM patients, which in turn may affect the placental function.

Keywords: gestational diabetes mellitus, miRNA-21, insulin resistance, PPAR-α

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]