Diagnostic Accuracy of Interleukin-27 in Bronchoalveolar Lavage Fluids for Pulmonary Tuberculosis
Received 16 September 2019
Accepted for publication 22 November 2019
Published 2 December 2019 Volume 2019:12 Pages 3755—3763
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Melinda Thomas
Peer reviewer comments 2
Editor who approved publication: Professor Suresh Antony
Shan Lin,1,2,* Yan Wang,1,* Yuhong Li,1 Di Xiao,1 Jin Guo,1 Weixiu Ma,1 Wenjing An,1 Hongqian Liu,1 Yingqing Shi,1 Lei Zhang,1 Jingxia Cui,1 Wei Guan1,3,*
1Department of Respiratory Medicine, Qinghai University Affiliated Hospital, Xining 810001, People’s Republic of China; 2Department of Medical Intensive Care Unit, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, GuangDong 510080, People’s Republic of China; 3Department of Respiratory and Critical Care Medicine, Baoan Central Hospital of Shenzhen/The Fifth Affiliated Hospital of Shenzhen University, Shenzhen, GuangDong 518102, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Wei Guan
Department of Respiratory and Critical Care Medicine, Baoan Central Hospital of Shenzhen/The Fifth Affiliated Hospital of Shenzhen University, 60 Xixiang Leyuan Street, Shenzhen, GuangDong 518102, People’s Republic of China
Department of Respiratory Medicine, Qinghai University Affiliated Hospital, Xining 810001, People’s Republic of China
Background: The World Health Organization states that China had 0.9 million cases of tuberculosis in 2017, accounting for 9% of cases globally. Despite a decrease in the incidence and mortality of tuberculosis in China over time, development in choosing the appropriate prevention and control of TB is required.
Purpose: The aim of this study was to evaluate the diagnostic significance of interleukin-27 in bronchoalveolar lavage fluids for pulmonary tuberculosis.
Materials and methods: Eventually, 107 bronchoalveolar lavage fluids from patients were included in this study. The concentrations of interleukin-27 and adenosine deaminase were determined in bronchoalveolar lavage fluids using enzyme-linked immunosorbent assay.
Results: It was found that the concentrations of interleukin-27 in bronchoalveolar lavage fluids of sputum-positive pulmonary tuberculosis group were significantly higher than those in sputum-negative pulmonary tuberculosis, lung cancer, and previous pulmonary tuberculosis groups, respectively (all P<0.001). Interleukin-27 levels in bronchoalveolar lavage fluids could be used for diagnostic purpose for pulmonary tuberculosis, with the cutoff value of 7.867 pg/mL; interleukin-27 had a sensitivity of 68.8% and specificity of 100% for the differential diagnosis of pulmonary tuberculosis (sputum-negative and sputum-positive PTB) from lung cancer. And with the cutoff value of 6.012 pg/mL, IL-27 had sensitivity and specificity of both 100% for the differential diagnosis of PTB from previous PTB. The risk of pulmonary tuberculosis was positively associated with the concentrations of interleukin-27 and adenosine deaminase in bronchoalveolar lavage fluids.
Conclusion: Interleukin-27 in bronchoalveolar lavage fluids is a sensitive and specific biomarker for the differential diagnosis of pulmonary tuberculosis from lung cancer and previous pulmonary tuberculosis.
Keywords: pulmonary tuberculosis, interleukin-27, adenosine deaminase, bronchoalveolar lavage fluid
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