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Correlations of lncRNAs with cervical lymph node metastasis and prognosis of papillary thyroid carcinoma

Authors Li N, Cui M, Yu P, Li Q

Received 22 October 2018

Accepted for publication 17 January 2019

Published 18 February 2019 Volume 2019:12 Pages 1269—1278

DOI https://doi.org/10.2147/OTT.S191700

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Amy Norman

Peer reviewer comments 2

Editor who approved publication: Dr Arseniy Yuzhalin


Na Li,1,2 Mingming Cui,2 Ping Yu,1 Qiang Li1

1Department of Endocrinology and Metabolism, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150080, China; 2Department of Internal Medicine, Harbin Medical University Cancer Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, China

Background: Cervical lymph node metastasis is an important prognostic indicator for papillary thyroid carcinoma (PTC) and affects treatment strategies for PTC. lncRNAs essentially contribute to the biological functions of tumors. This study aimed to identify the lncRNAs associated with cervical lymph node metastasis and prognosis of PTC and their potential pathophysiological mechanisms.
Materials and methods: PTC-associated lncRNAs were selected from The Cancer Genome Atlas database, and correlations among lncRNAs, lymph node metastasis, tumor staging, and prognosis of PTC were analyzed in silico. These correlations were then validated through quantitative reverse transcription PCR (qRT-PCR) and immunohistochemistry (IHC).
Results: In silico analysis showed that FAM95B1 and UCA1 were significantly correlated with cervical lymph node metastasis, tumor staging, and PTC prognosis (P<0.05). qRT-PCR analysis revealed high UCA1 expression in PTC tissues and correlations between UCA1 expression levels and cervical lymph node metastasis and tumor staging in PTC, that is, higher UCA1 expression resulted in poorer PTC prognosis. IHC analysis revealed that a high expression of UCA1 was accompanied by a high expression of metastasis-related proteins (MMP-2 and MMP-9), thereby validating the correlation of UCA1 expression with metastasis.
Conclusion: FAM95B1 and UCA1 expression was significantly correlated with the occurrence and progression of PTC. The expression levels of UCA1 significantly affected the prognosis of PTC patients and were significantly correlated with tumor staging and cervical lymph node metastasis.

Keywords: papillary thyroid carcinoma, lncRNA, FAM95B1, UCA1

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