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Comprehensive Analysis of SiNPs on the Genome-Wide Transcriptional Changes in Caenorhabditis elegans

Authors Liang S, Duan J, Hu H, Zhang J, Gao S, Jing H, Li G, Sun Z

Received 25 February 2020

Accepted for publication 10 June 2020

Published 23 July 2020 Volume 2020:15 Pages 5227—5237

DOI https://doi.org/10.2147/IJN.S251269

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Mian Wang


Shuang Liang,1,2,* Junchao Duan,1,2,* Hejing Hu,1,2 Jingyi Zhang,1,2 Shan Gao,2,3 Haiming Jing,2,3 Guojun Li,2,3 Zhiwei Sun1,2

1Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing 100069, People’s Republic of China; 2Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, People’s Republic of China; 3Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control/Beijing Center of Preventive Medicine Research, Beijing 100013, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Zhiwei Sun
School of Public Health, Capital Medical University, Beijing 100069, People’s Republic of China
Email zwsun@ccmu.edu.cn
Guojun Li
Beijing Center for Disease Prevention and Control/Beijing Center of Preventive Medicine Research, Beijing 100013, People’s Republic of China
Email ligj@bjcdc.org

Background: Large-scale production and application of amorphous silica nanoparticles (SiNPs) have enhanced the risk of human exposure to SiNPs. However, the toxic effects and the underlying biological mechanisms of SiNPs on Caenorhabditis elegans remain largely unclear.
Purpose: This study was to investigate the genome-wide transcriptional alteration of SiNPs on C. elegans.
Methods and Results: In this study, a total number of 3105 differentially expressed genes were identified in C. elegans. Among them, 1398 genes were significantly upregulated and 1707 genes were notably downregulated in C. elegans. Gene ontology analysis revealed that the significant change of gene functional categories triggered by SiNPs was focused on locomotion, determination of adult lifespan, reproduction, body morphogenesis, multicellular organism development, endoplasmic reticulum unfolded protein response, oocyte development, and nematode larval development. Meanwhile, we explored the regulated effects between microRNA and genes or signaling pathways. Pathway enrichment analysis and miRNA-gene-pathway-network displayed that 23 differential expression microRNA including cel-miR-85-3p, cel-miR-793, cel-miR-241-5p, and cel-miR-5549-5p could regulate the longevity-related pathways and inflammation signaling pathways, etc. Additionally, our data confirmed that SiNPs could disrupt the locomotion behavior and reduce the longevity by activating ins-7, daf-16, ftt-2, fat-5, and rho-1 genes in C. elegans.
Conclusion: Our study showed that SiNPs induced the change of the whole transcriptome in C. elegans, and triggered negative effects on longevity, development, reproduction, and body morphogenesis. These data provide abundant clues to understand the molecular mechanisms of SiNPs in C. elegans.

Keywords: SiNPs, genome microarrays, longevity, development, Caenorhabditis elegans

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