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Columbamine suppresses the proliferation and malignization of colon cancer cells via abolishing Wnt/β-catenin signaling pathway

Authors Lei C, Yao Y, Shen B, Liu J, Pan Q, Liu N, Li L, Huang J, Long Z, Shao L

Received 24 March 2019

Accepted for publication 22 July 2019

Published 23 September 2019 Volume 2019:11 Pages 8635—8645

DOI https://doi.org/10.2147/CMAR.S209861

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Cristina Weinberg

Peer reviewer comments 2

Editor who approved publication: Dr Antonella D'Anneo


Changjiang Lei,1,* Yao Yao,2,* Bin Shen,3,* Junru Liu,2,* Qingyun Pan,4,* Ning Liu,1 Lei Li,1 Jianbin Huang,1 Zhixiong Long,2 Liwei Shao1

1Department of General Surgery, Fifth Hospital in Wuhan, Wuhan, Hubei 430050, People’s Republic of China; 2Department of Oncology, The Fifth Hospital of Wuhan, Wuhan, Hubei 430050, People’s Republic of China; 3Department of Hepatobiliary Surgery, Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing 314001, Zhejiang, People’s Republic of China; 4Department of Blood Endocrinology, The Fifth Hospital of Wuhan, Wuhan, Hubei 430050, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Zhixiong Long
Department of Oncology, The Fifth Hospital of Wuhan, No. 122, Xian Zheng street, Hanyang District, Wuhan 430000, Hubei, People’s Republic of China
Tel +86 1 850 719 9619
Email 2428850630@qq.com
 
Liwei Shao
Department of General Surgery, The Fifth Hospital of Wuhan, No. 122, Xian Zheng street, Hanyang District, Wuhan 430000, Hubei, People’s Republic of China
Tel +86 1 387 105 8697
Email b18310715028@163.com

Background: Colon cancer is one of the most common malignancies worldwide. Because of the side effects and defects in tolerance of chemotherapy, it is necessary to discover new drugs for colon cancer treatment. Columbamine has been identified as an effective anti-osteosarcoma compound with only minor side effects. In this study, we analyzed the anticancer effect of columbamine on colon cancer.
Methods: Human colon cancer cell lines were treatment with columbamine. MTT assay, colony formation assay, apoptosis detection and tumorigenicity assay were performed to detect the protective effect of columbamine on colon cancer development. Western blot assay and luciferase reporter assay were conducted to investigate the potential mechanism of the columbamine treatment.
Results: Columbamine significantly inhibited the proliferation, migration, invasion process of colon cancer cells, and dramatically promoted the apoptosis rate of colon cancer cells to further suppress the development of colon cancer to tumor. Both the signaling transducing and key factors expression of Wnt/β-catenin signaling pathway were obviously repressed by columbamine treatment in a dose-dependent manner.
Conclusion: The present study indicated that columbamine exerts its anti-tumor effect in colon cancer cells through abolishing Wnt/β-catenin signaling pathway. Columbamine may be a new therapy compound for colon cancer.

Keywords: colon cancer, cells growth, apoptosis, HCT116, LoVo

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