circPCNX and Pecanex Promote Hepatocellular Carcinoma Cell Viability by Inhibiting miR-506
Authors Sun P, Fan X, Hu X, Fu X, Wei Q, Zang Y
Received 30 September 2019
Accepted for publication 18 December 2019
Published 3 January 2020 Volume 2019:11 Pages 10957—10967
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 2
Editor who approved publication: Dr Chien-Feng Li
Peng Sun,1,* Xinyi Fan,2,* Xiao Hu,3 Xiaoyue Fu,1 Qian Wei,3 Yunjin Zang1
1Department of Liver Transplantation, The Affiliated Hospital of Qingdao University, Qingdao 266003, People’s Republic of China; 2Intensive Care Unit, the Affiliated Hospital of Qingdao University, Qingdao 266003, People’s Republic of China; 3Department of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qingdao University, Qingdao 266003, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Yunjin Zang
Department of Liver Transplantation, The Affiliated Hospital of Qingdao University, 69 Haier Road, Qingdao 266003, People’s Republic of China
Tel/Fax +86 532 82915906
Introduction: Hepatocellular carcinoma (HCC) is one of the most common malignant cancers, while the molecular mechanism is not clear. Circular RNAs (circRNAs) are a class of naturally occurring endogenous noncoding RNAs that prove to play important roles in the occurrence, development and prognosis of HCC. In this study, we focused on an abnormally expressed circular RNA-circPCNX in HCC and study the function of circPCNX and Pecanex (PCNX) in HCC.
Methods: Circular RNA sequencing was used to find the abnormally expressed circRNAs and qRT-PCR was used to verify it. CCK8 assay, colony formation assay and cell apoptosis assay were used to study biological functions, and Luciferase reporter assay and Western blot analysis were used to study the mechanism.
Results: We observed that circPCNX and Pecanex were significantly upregulated in tumor tissues of patients with HCC and correlated with clinicopathological variables or prognosis of HCC patients. Functional investigations showed circPCNX and Pecanex could promote the viability of HCC cells. Mechanistic investigations suggested that both circPCNX and Pecanex 3ʹUTR could bind to miR-506 and subsequently inhibited the miR-506-induced anticarcinogenic effect in HCC.
Conclusion: Our study revealed the function of circPCNX and Pecanex in promoting HCC progression and acting as biomarkers in the clinical estimate and treatment of HCC.
Keywords: circPCNX, Pecanex, miR-506, hepatocellular carcinoma
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.Download Article [PDF] View Full Text [HTML][Machine readable]