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Characterising Vascular Cell Monolayers Using Electrochemical Impedance Spectroscopy and a Novel Electroanalytical Plot

Authors Bussooa A

Received 17 June 2020

Accepted for publication 27 August 2020

Published 23 September 2020 Volume 2020:13 Pages 89—101

DOI https://doi.org/10.2147/NSA.S266663

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Professor Israel (Rudi) Rubinstein


Anubhav Bussooa

BHF Cardiovascular Research Centre, University of Glasgow, Glasgow G12 8TA, UK

Correspondence: Anubhav Bussooa
BHF Cardiovascular Research Centre, University of Glasgow, Glasgow G12 8TA, UK
Email abussooa@gmail.com

Introduction: Biological research relies on the culture of mammalian cells, which are prone to changes in phenotype during experiments involving several passages of cells. In regenerative medicine, specifically, there is an increasing need to expand the characterisation landscape for stem cells by identifying novel stable markers. This paper reports on a novel electric cell-substrate impedance sensing-based electroanalytical diagram which can be used for the “electrical characterisation” of cell monolayers consisting of smooth muscle cells, endothelial cells or co-culture.
Materials and Methods: Interdigitated electrodes were microfabricated using standard cleanroom procedures and integrated into cell chambers. Electrochemical impedance spectroscopy data were acquired for 2 vascular cell types after they formed monolayers on the electrodes.
Results and Discussion: A Mean impedance per unit area vs Mean phase plots provided a reproducible, visually obvious and statistically significant method of characterising cell monolayers. This electroanalytic diagram has never been used in previous papers, but it confirms findings by other research groups using similar approaches that the complex impedance spectra of different cell type are different. Further work is required to determine whether this method could be extended to other cell types, and if this is the case, a library of “signature spectra” could be generated for “electrical characterisation” of cells.

Keywords: impedance sensors, electrochemical impedance spectroscopy, Bode plot, Nyquist diagram, electroanalytical plot

Creative Commons License This work is published by Dove Medical Press Limited, and licensed under a Creative Commons Attribution License. The full terms of the License are available at http://creativecommons.org/licenses/by/4.0/. The license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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