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Bioactive chromone constituents from Vitex negundo alleviate pain and inflammation

Authors Khan A, Naz S, Farooq U, Shahid M, Ullah I, Ali I, Rauf A, Mabkhot YN

Received 4 July 2017

Accepted for publication 26 October 2017

Published 28 December 2017 Volume 2018:11 Pages 95—102


Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Katherine Hanlon

Ajmal Khan,1,2 Sadia Naz,1 Umar Farooq,1 Muhammad Shahid,3 Irfan Ullah,4 Iftikhar Ali,5 Abdur Rauf,6 Yahia Nasser Mabkhot7

1Department of Chemistry, COMSATS Institute of Information Technology, Abbottabad, Pakistan; 2University of Nizwa Chair of Oman’s Medicinal Plants and Marine Natural Products, University of Nizwa, Nizwa, Oman; 3Department of Pharmacy, Sarhad University of Science and Information Technology, 4Department of Pharmacy, University of Peshawar, Peshawar, 5Department of Chemistry, Karakoram International University, Gilgit-Baltistan, 6Department of Chemistry, University of Swabi, Khyber Pakhtunkhwa, Pakistan; 7Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia

Background: Vitex negundo L. has been widely studied for its beneficial effect in inflammatory and pain conditions. The present study describes the isolation of two new bioactive chromone constituents from V. negundo and their in vivo evaluation for anti-inflammatory and antinociceptive activities.
Methods: Two new chromone derivatives, namely, methyl 3-(2-(5-hydroxy-6-methoxy-4-oxo-4H-chromen-2-yl)ethyl)benzoate (1) and 3-(1-hydroxy-2-(5-hydroxy-6-methoxy-4-oxo-4H-chromen-2-yl)ethyl)benzoic acid (2) were isolated from V. negundo and their structures were determined through various spectroscopic techniques including mass spectrometry, UV, IR, 1H NMR, 13C NMR, and two-dimensional-NMR like correlation spectroscopy and heteronuclear multiple bond correlation techniques. The isolated compounds (12) were tested for their prospective antinociceptive activity in acetic acid-induced abdominal constriction assay and anti-inflammatory activity in the carrageenan-induced paw edema assay in mice.
Results: Significant attenuation (P<0.001) of tonic visceral nociception was demonstrated by compound 1 and 2 at doses of 50 and 100 mg/kg. At similar doses, these compounds (12) also showed potent amelioration (P<0.001) of carrageenan-induced paw swelling.
Conclusion: The isolated chromone derivatives (12) from V. negundo are able to alleviate nociception and inflammation and the findings corroborated that V. negundo may be used as a potential source of antinociceptive and anti-inflammatory candidates.

Keywords: chromone derivatives, Vitex negundo, Verbenaceae, antinociceptive, anti-inflammatory

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