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B7-H3 expression in breast cancer and upregulation of VEGF through gene silence

Authors Sun J, Guo Y, Li X, Zhang Y, Gu L, Wu P, Bai G, Xiao Y

Received 3 March 2014

Accepted for publication 26 June 2014

Published 29 October 2014 Volume 2014:7 Pages 1979—1986

DOI https://doi.org/10.2147/OTT.S63424

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 4


Jing Sun,1 Yun-Di Guo,1 Xiao-Ning Li,1 Yang-Qin Zhang,1 Li Gu,1 Ping-Ping Wu,1 Guang-Hui Bai,2 Yang Xiao1

1Suzhou Health College, Jiangsu Suzhou, People’s Republic of China; 2The Fourth Hospital of Suzhou, Jiangsu Suzhou, People’s Republic of China


Abstract: B7-H3, a novel member of the B7 family, was previously known as a regulatory ligand regulating T-cell-mediated immune response, and in recent years it was found to take a significant role in various cancers. In some tumor types, high expression of B7-H3 had been linked to a poor prognosis, whereas in other cancers the opposite effect had been observed. The precise role of B7-H3 in tumor immunity is unclear, and further investigations are needed. In the present study, we studied the expression of B7-H3 in the pathologic specimens of 221 patients treated for breast cancer by immunohistochemistry. Strong B7-H3 expression was found in cancer tissues from 80.55% patients, and B7-H3 expression had a negative relation with vascular endothelial growth factor (VEGF) expression, microvascular density for CD34, and tumor size. Furthermore, through lipopolysaccharide-mediated delivery of stable short hairpin ribonucleic acid we observed that silencing of B7-H3 could increase the transcription and secreting of VEGF in breast cancer cell line MCF-7. In summary, the present study demonstrated that B7-H3 suppressed tumor growth through inhibiting VEGF expression. These results increased knowledge of the nonimmunological role of B7-H3 protein and provided novel insights into great biological functions and a putative therapeutic target in breast cancer.

Keywords: breast cancer, B7-H3, vascular endothelial growth factor

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