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Antimicrobial resistance pattern and molecular genetic distribution of metallo-β-lactamases producing Pseudomonas aeruginosa isolated from hospitals in Minia, Egypt

Authors Farhan SM, Ibrahim RA, Mahran KM, Hetta HF, Abd El-Baky RM

Received 15 December 2018

Accepted for publication 17 April 2019

Published 16 July 2019 Volume 2019:12 Pages 2125—2133

DOI https://doi.org/10.2147/IDR.S198373

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Colin Mak

Peer reviewer comments 2

Editor who approved publication: Dr Joachim Wink


Sara M Farhan,1 Reham A Ibrahim,2 Khaled M Mahran,3 Helal F Hetta4,5, Rehab M Abd El-Baky1,2

1Department of Microbiology and Immunology, Faculty of Pharmacy, Deraya University, Minia 11566, Egypt; 2Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia 61519 Egypt; 3General Surgery and Laparoscopic Surgery, Faculty of Medicine, Minia University, Minia 61519 Egypt; 4Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71515, Egypt; 5Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA

Background: Pseudomonas aeruginosa (P. aeruginosa) represents a great threat to public health worldwide, due to its high ability to acquire resistance to different antibiotic classes. Carbapenems are effective against multidrug resistant (MDR) P. aeruginosa, but their widespread use has resulted in the emergence of carbapenem-resistant strains, which is considered a major global concern. This study aimed to determine the prevalence of carbapenem resistance among P. aeruginosa strains isolated from different sites of infection.
Methods: Between October 2016 and February 2018, a total of 530 clinical specimens were collected from patients suffering from different infections, then processed and cultured. Isolates were tested for extended spectrum β-lactamase (ESBL) and metallo-β-lactamase (MBL) production using double-disk synergy test, modified Hodge tests, and disc potentiation test. PCR was used for the detection of selected OXA carbapenemases encoding genes.
Results: Of 530 samples, 150 (28.3%) P. aeruginosa isolates were obtained. MDR strains were found in 66.6% (100 of 150) of isolates. Of 100 MDR P. aeruginosa isolates, 54 (54%) were ESBL producers and 21 (21%) carbapenem resistant P. aeruginosa. MBL production was found in 52.3% (eleven) carbapenem-resistant isolates. CTX-M15 was found among 55.5% of ESBL- producing P. aeruginosa. Carbapenemase genes detected were blaIMP (42.8%, nine of 21), blaVIM (52.3%, eleven of 21), blaGIM (52.3%, eleven of 21), blaSPM (38%, 8/21). In addition, isolates that were positive for the tested genes showed high resistance to other antimicrobials, such as colistin sulfate and tigecycline.
Conclusion: Our study indicates that P. aeruginosa harboring ESBL and MBL with limited sensitivity to antibiotics are common among the isolated strains, which indicates the great problem facing the treatment of serious infectious diseases. As such, there is a need to study the resistance patterns of isolates and carry out screening for the presence of ESBL and MBL enzymes, in order to choose the proper antibiotic.

Keywords: MDR; P. aeruginosa, ESBL, MBL, antimicrobial resistance

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