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Anti-tumor efficacy of folate modified PLGA-based nanoparticles for the co-delivery of drugs in ovarian cancer

Authors Li S, Li X, Ding J, Han L, Guo X

Received 21 November 2018

Accepted for publication 14 March 2019

Published 18 April 2019 Volume 2019:13 Pages 1271—1280


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Cristiana Tanase

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Shu Li, Xiaofeng Li, Jianyi Ding, Lingfei Han, Xiaoqing Guo

Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, People’s Republic of China

Background: Ovarian cancer is a leading cause of death in gynecologic malignancies. The high mortality is mainly caused by advanced stage at presentation in most patients. Even after the combination of cytoreductive surgery and systemic platinum and taxane treatment, most patients relapse and eventually succumb to the disease. Therefore, there is an urgent need for new treatments.
Purpose: A novel folate (FA)-targeted co-delivery of docetaxel (DTX) and gemcitabine (GEM) nanoparticles (NPs) was developed to overcome ovarian cancer.
Materials and methods: Physicochemical characteristics of NPs such as size, morphology, and release profiles were explored. In vitro and in vivo studies were carried out to assess the efficacy of their antitumor activity in target cells.
Results: FA modified DTX and GEM co-loaded NPs were prepared using the solvent evaporation method. The NPs with a particle size of ∼120nm were stable in the observation period. The hemolysis results indicated that FA-PEG2000-PLGA was potentially feasible for targeted antitumor drug delivery through blood circulation. In vitro release study suggested that in comparison with the free drug, PLGA-DTX/GEM NPs and FA-PEG2000-PLGA-DTX/GEM NPs had sustained-release properties. However, there was no obvious difference between the two NPs with the same drug in the release profile. Ovarian cancer cells in vitro efficiently took up the non-targeted and FA-targeted NPs; improved cytotoxicity was observed in the FA-targeted NPs, showing a 3.59- fold drop in the IC50 in SKOV-3 cells as compared to DTX/GEM alone. Cellular uptake showed that through surface modification, more drugs entered the cell successfully. Pharmacodynamics results showed a statistically significant effect on the rate of reduction of tumor volume for FA-PEG2000-PLGA-DTX/GEM NPs than other groups and no toxicity of organs.
Conclusion: The present study indicates that the FA-PEG2000-PLGA-DTX/GEM NPs provides a promising platform for the treatment of ovarian cancer.

Keywords: folate, co-delivery, docetaxel, gemcitabine, nanoparticles


Ovarian cancer is a leading cause of death in gynecologic malignancies.1 The high mortality is mainly caused by advanced stage at presentation in most patients. Even after the combination of cytoreductive surgery and systemic platinum and taxane treatment, most patients relapse and eventually succumb to the disease.2 Therefore, there is an urgent need for new treatments. Docetaxel (DTX) is a member of the taxane family. It has been shown to be effective for a variety of malignant tumors including ovarian cancer.3 However, the shortcomings of DTX, such as poor water solubility, low oral bioavailability and serious adverse reactions, have a negative impact on its clinical application. Side effects, including allergic reactions, fluid retention, neuromusculoskeletal toxicity and neutropenia, may be resulted from the fact that active pharmaceutical ingredients (API) or components of the solvent system need to dissolve hydrophobic DTX, such as Tween 80 or ethanol, which are associated with the formula of those currently in the market. Gemcitabine (GEM) is an S-phase-specific anti-metabolite with shown activity in a range of tumor types, especially those influencing the pancreas, lung and bladder.4

When GEM and other chemotherapeutic agents are used in combination, different modes of action lead to increased possibility of cytotoxic activity. In fact, in vivo experiments in murine tumors have shown the additive and super additive activity (mainly through the enhancement of induced apoptosis) when DTX and GEM are combined in different sequences.5 In addition, synergistic cytotoxicity has been reported in a human cancer cell line after administration of GEM followed by DTX 96 h later.6 Some pharmaceutical experts have prepared DTX and GEM co-delivery preparation platforms, but unfortunately they are limited to the study of breast cancer.79

Folate receptor (FA) is a glycosylated phosphatidylinositol binding membrane glycoprotein widely distributed in normal and tumor tissues. α type folic acid receptor is the main subtype of folate transport, and is over expressed on the surface of various types of tumor, including pancreatic, prostate, lung, head and neck, breast and ovarian cancers, as well as mesothelioma.10,11 Over 90% of ovarian cancer cells overexpress folate receptors.12

Based on the above observations, we have studied the development of folic acid-targeted co-delivery PLGA nanoparticles of DTX and GEM (FA-PLGA-DTX/GEM NPs) in this study. The prepared NPs were characterized for drug loading and encapsulation efficiency, particle size, and surface morphology. Furthermore, the in vitro release profiles, cellular uptake and effect on cell viability in SKOV3 ovarian cancer cell lines of NPs were evaluated.

Materials and methods


DTX and GEM were gifted by Hengrui Pharmaceuticals Co Ltd (Jiangsu, China). FA-PEG2000-PLGA or PLGA was purchased from Hunan Huaten Pharma (Changsha, China). SKOV3 ovarian cancer cell lines were purchased from the Shanghai Institute of Biochemistry and Cell Biology. The chemical and solvents used were analytical or HPLC. In this study, deionized water was used. Female Balb/c mice (5–6 weeks old, 25±2 g) were obtained from Laboratory Animal Center of Faculty of Pharmacy and Pharmaceutical Science, Tongji University School of Medicine, China. All animals were free from pathogens and had free access to food and water. Animal experiments were carried out in accordance with the guidelines issued by the National Institutes of Health and approved by the Tongji University School of Medicine.

Preparation of FA-PEG2000-PLGA-DTX/GEM NPs

FA modified DTX and GEM co-loaded NPs were prepared using the solvent evaporation method. Briefly, DTX (5 mg), GEM (5 mg) and FA-PEG2000-PLGA (100 mg) were dissolved in 10 mL of chloroform to produce oil phase. This was attached to a rotary evaporator and the organic phase was removed by evaporation at 45±2 °C to form a film on the flask wall. The flask was kept overnight to remove any trace in the solvent. The lipid membranes were hydrated with 5 mL of phosphate-buffered saline (PBS, pH 7.4) at 37 °C for 30 mins. After that, the suspension was mixed and homogenized for 10 cycles at 25,000 psi to obtain NPs preparations (Figure 1A). The suspensions in ultrafilter tubes (MWCO, 30,000 Da) were centrifuged at 1,000×g for 30 min to remove free drug. Non-targeted preparations (PLGA-DTX/GEM NPs) without a folate conjugate and corresponding blank NPs without the DTX/GEM were prepared in a similar manner. The ultimate solution was filtered through a 0.8 µm cellulose acetate filter unit, freeze-dried, and stored at −20 °C until use.

Figure 1 The microstructural schematic diagram of FA-PEG2000-PLGA-DTX/GEM NPs (A). Transmission electron microscope photograph of FA-PEG2000-PLGA-DTX/GEM NPs (B) and PLGA-DTX/GEM NPs (C). (amplification×44,000).Abbreviations: DTX, docetaxel; GEM, gemcitabine; NPs, nanoparticles.


Morphologies of NPs were observed by TEM (H-600; Hitachi, Tokyo, Japan) with an accelerating voltage of 75 kV. A droplet of copolymer solution (0.5 mg/mL) was deposited on a carbon-coated copper grid (200 meshes). A picture of the sample was taken after it was completely air-dried. Size distribution and zeta potential were determined by diluting 10 μl of different NPs formulations in 10 ml of deionized water and subjected to analysis using Zetas Sizer ZS (Malvern, Worcestershire, UK). The average size of each NPs formulation was determined by dynamic light scattering at room temperature and a fixed angle of 90°. The polydispersity index (PDI) of the droplets was determined for a count rate between 100–500 kcps. For zeta potential measurements, the diluted sample was placed in an electrophoresis tank and the charge was recorded.

Entrapment efficiency and drug loading

Lyophilized powder of FA-PEG2000-PLGA-DTX/GEM NPs was dispersed in an aqueous medium containing 0.5% (w/v) Tween-80 to dissolve DTX and GEM. After centrifugation at 14,000 rpm for 15 mins (Eppendorf Centrifuge 5430; Hamburg, Germany), the free drug was separated from NPs by an ultracentrifugal filter (cut-off 10,000 Da). The amount of free drug was determined by HPLC. The entrapment efficiency and drug loading were calculated as follows:

where Mdrug is the mass of drug loaded in nanoparticles, Mpolymer the mass of polymer in formulations, and Madded the mass of added drug.

Stability study

The accelerated stability study was conducted according to the International Coordinating Committee (ICH) guidelines (1993). The freshly prepared freeze-dried NPs were placed in a stable chamber at 25 °C, 60% RH. The NPs subjected to stability tests were analyzed over a 3-month period for physical appearance, size, physical and chemical properties and with a sampling frequency of 1 month.

Hemolytic activity testing

Hemolytic activity was evaluated by determining hemoglobin release from erythrocyte after incubation with different DTX/GEM NPs preparations. Simply put, blood from the rabbit ear artery was collected into tubes containing 124 mM sodium citrate (sodium citrate: blood =1: 9, v/v), centrifuged and washed with saline. The obtained red blood cells (RBC, 1 mL) were diluted with saline to 10 mL. 0.5 mL of the RBC suspension was incubated with different concentration NPs (Final Concentration=0.02, 0.05, 0.15 mg/mL) at 37 ºC with gentle shaking. After 1 hr, the samples were centrifuged for 5 min at 3000 rpm and the absorbance (A) of the supernatant was determined by UV-vis spectrophotometry at 545 nm. 0.5 mL of the RBC suspension with 2 mL of saline (0% lysis) was prepared as a negative control while water was used as a positive control (100% lysis). The positive absorbance value should be 0.8±0.3, and the negative should be less than 0.03. The hemolysis rate of the sample was calculated by the following equation:

Among them, At represents absorbance value of test sample, Anc and Apc represent the absorption values of negative and positive control respectively.

In vitro release profiles

In vitro release of drug from the FA-PEG2000-PLGA-DTX/GEM NPs was studied in an aqueous PBS medium (pH 7.4, containing 0.5% Tween-80 (w/v)). Briefly, an aliquot of NPs, containing a fixed amount of DTX or GEM (10 mg), was introduced into a dialysis bag and then placed in PBS adjusted at 37 °C under gentle magnetic stirring (75 rpm) in a volume of 200 ml to achieve the sink condition. Drug release from different NPs went on for 24 hrs, and 1 ml samples were withdrawn at predetermined time intervals and replaced with fresh medium. The concentrations of DTX or GEM in samples were analyzed by HPLC. The whole process of the experiment was performed in triplicate. The release of free drugs and PLGA-DTX/GEM NPs was also carried out in the same way as the control samples.

Cytotoxicity assay

The cytotoxicity of blank NPs, free DTX/GEM, PLGA-DTX/GEM NPs, and FA-PEG2000-PLGA-DTX/GEM NPs was evaluated by MTT assay in SKOV-3 cells. Briefly, the cells were maintained in RPMI 1640 culture medium supplemented with 10% FBS and 1% penicillin (100 IU/mL)-streptomicin (100 μg/mL) under an incubation condition of 98% relative humidity and 5% CO2. Each cell line contained 6×104 cells/mL, and was seeded in 96-well cell culture plates and incubated for 24 hrs to ensure cell attachment under the above condition. Subsequently, the cells were treated with different samples: blank NPs, free DTX/GEM, PLGA-DTX/GEM NPs, and FA-PEG2000-PLGA-DTX/GEM NPs. Each sample was prepared with a different DTX/GEM concentration (ranging from 5.0 ng/mL to 1000 ng/mL, and plates were incubated for 24 hrs, DTX:GEM=1:1). Cells were then incubated with 20 µL MTT solution (5 mg/mL in PBS) for 4 h before the medium was removed, and 200 µL DMSO was used to dissolve cells. The absorbance was 570 and 490 nm as shown by the microplate reader (Thermo Fisher Scientific). The calculation formula of cell viability (%) is as follows:

A Test, A Control, and A Blank represent the absorbances of the cells with different treatments, untreated cells, and blank culture media respectively. Each experiment was performed three times.

Cellular uptake

Cellular uptake of the FA-PEG2000-PLGA-DTX/GEM NPs was quantitatively compared to non-targeted NPs using coumarin 6 as a fluorescent probe, which was entrapped instead of DTX/GEM in the NPs.17 Briefly, SKOV-3 cells were placed in a 12-well plate at a density of 2×105 cells per well . After 24 h of incubation, coumarin 6-loaded FA-PEG2000-PLGA NPs, coumarin 6-loaded PLGA NPs and free coumarin 6, with the same final coumarin-6 concentration of 0.5 µg/mL and diluted in culture medium, were applied to each well and incubated for a further 2 hrs. Then, cells were collected using trypsin-EDTA mixture (0.25%-0.01%), washed twice with PBS, and centrifuged at 1,800 rpm to remove any non-internalized particles. Finally, the cells were resuspended in PBS for flow-cytometry measurement using FACS Calibur (Becton Dickinson, Franklin Lakes, NJ, USA). In order to confirm that the fluorescence detected in flow-cytometry studies was resulted from the uptake of FA-PEG2000-PLGA-DTX/GEM NPs into the cells, free coumarin 6 was removed using dialysis bag (cutoff 12,000 Da).

Antitumor study

In the study of cancer treatment, each nude mouse was implanted with SKOV-3 cells (5×106 per mouse). When the initial average size of tumor reached 50~100 mm3, the mice were randomly divided into four groups (n=6): the control group (PBS), free DTX/GEM, PLGA-DTX/GEM NPs and FA-PEG2000-PLGA-DTX/GEM NPs. Then the mice were given a dose of 10 mg/kg via the tail vein every 2 days for 3 weeks. The umor size and the body weight were examined and recorded every 3 days. The volume of the tumor (V) was calculated according to the following formula:18 Tumor volume mm=Long diameter×Short diameter2×0.52. After treatment, the mice were sacrificed. The main organs and tumors were weighed and the tissue sections were collected. The specimens were fixed with 4% paraformaldehyde and then paraffin embedded for hematoxylin and eosin (H-E) staining and histological examination.

Statistical analysis

Student’s t-test was used to compare the two groups. P<0.05 was considered statistically significant. All data from the three independent experiments were expressed as the mean±SD.

Result and discussion


The morphology of NPs was observed visually by TEM. FA-PEG2000-PLGA-DTX/GEM NPs could be self-assembled into spherical NPs with a diameter of ~120 nm (Figure 1B). In contrast, PLGA-DTX/GEM NPs showed spherical morphology with a diameter of ~110 nm (Figure 1C). The surface potential of two NPs was −22.3±1.4 mV and −19.5±1.2 mV respectively. Interestingly, the slight increase in the diameter of FA-PEG2000-PLGA-DTX/GEM NPs and the slight reduction of PLGA-DTX/GEM NPs surface potential (absolute value) might be attributed to the hydrophilic ability and the surface charge-shielding effects of hydrophilic PEG outer layer. In the process of drug-delivery, physical coating and electrostatically interacted drug loading in carriers were expected to prevent premature drug loss. A favorable hydrodynamic diameter (around 100 nm)13 and a negative surface charge14 were important in meeting the needs of advanced antitumor drug-delivery.

Drug entrapment efficiency (DTX) in FA-PEG2000-PLGA-DTX/GEM NPs was 81.3%±3.3%, slightly lower than the non-targeted PLGA-DTX/GEM NPs (84.9%±6.4%). Other parameters are shown in Table 1.

Table 1 Characterization and stability study of the FA-PEG2000-PLGA-DTX/GEM NPs and PLGA-DTX/GEM NPs. (n=3; mean± SD)

Stability study

FA-PEG2000-PLGA-DTX/GEM NPs exhibited good stability during the three months. No significant changes in physical appearance or particle aggregation were observed. At the same time, NPs showed good physical stability. There was no obvious change in all the indexes during the observation period. No degradation was observed in DL% and ER% in the preparations (Table 1).

Hemolytic activity testing

Despite all these advantages in size and surface properties, intravenous injection of NPs always came with potential risks, such as causing severe erythrocyte damage known as hemolytic activity (lysis of red blood cells). Even excessive hemolysis activity could be seriously life-threatening.15,16 Therefore, the study of hemolytic activity is one of the important safety endeavors to prevent serious side effects in vivo. Although in practice, polymer concentration applied in blood is diluted, in order to ensure adequate safety, an excessively high polymer concentration in this study was examined.

Hemolytic images showed no obvious hemolysis in any polymer concentration (Figure 2). Although hemolysis increased slightly at a higher polymer concentration, that of PLGA-DTX/GEM NPs and FA-PEG2000-PLGA-DTX/GEM NPs was less than 5%, which is considered to be non-toxic in in vivo applications. Low hemolysis indicated good biocompatibility and low toxicity even when the polymer concentration was as high as 0.15 mg/ml. Therefore, the hemolysis results indicated that FA-PEG2000-PLGA was potentially feasible for targeted antitumor drug delivery through blood circulation.

Figure 2 Hemolytic activity results (B) and photo images (A) at different polymeric concentrations.

In vitro release profiles

The cumulative drug-release profile in vitro was shown in Figure 3. Over time, the release of DTX and GEM in NPs became much slower than that of free drug. Within 2 hrs, the cumulative release of free drug (DTX) was about 92% but only about 45% in NPs (GEM 82%, 22%). In this case, biphasic release was observed with DTX to be about 45% within 2 hrs, followed by a sustained release of about 77% within 24 hrs (GEM 22%, 60%). The main reason for this phenomenon was that the drug loaded on the NPs was easier to release into the dissolution medium at first; that the NPs dissolved over time, and that the drugs within the particles were gradually released. It suggested that in comparison with the free drug, PLGA-DTX/GEM NPs and FA-PEG2000-PLGA-DTX/GEM NPs had better sustained-release properties. However, there was no obvious difference between the two NPs with the same drug in the release profile. After calculation, the in vitro drug-release kinetic model of DTX and GEM (FA-PEG2000-PLGA-DTX/GEM NPs) in release medium was found to fit well with the Higuchi equation: Q=7.12t1/2-1.23 (DTX, r=0.994) and Q=4.32t1/2+0.32 (GEM, r=0.993). Thus, it was speculated that the sustained-release properties of NPs could extend the absorption time of drugs in vivo.

Figure 3 In vitro release profiles of DTX and GEM from different nanoparticles. Release experiments were carried out in PBS as a dissolution medium at 37±0.5◦C. Each point represents the mean value of three different mean±SD. (n=3).Abbreviations: DTX, docetaxel; GEM, gemcitabine; NPs, nanoparticles.

Cytotoxicity assay

In order to investigate the in vitro cytotoxicity of FA-PEG2000-PLGA-DTX/GEM NPs in comparison to free DTX/GEM and PLGA-DTX/GEM NPs, MTT assay was performed on SKOV-3 cells. Blank NPs, which did not contain DTX/GEM were used as controls. As shown in Figure 4, significant reductions in cell viability were observed in the FA-PEG2000-PLGA-DTX/GEM NPs treated group, while the cytotoxicity of cells was clearly lower in the PLGA-DTX/GEM NPs treated and free DTX/GEM groups. In addition, blank NPs without DTX/GEM showed no apparent cytotoxicity to SKOV-3 cells, even at the highest concentration used (1000 ng/mL). According to the above results, although DTX and GEM were powerful antitumor drugs, they could not completely inhibit cell proliferation. Similarly, NPs conjugation of PTX/CUR did not result in superior effect. The ability to suppress tumor cells was only slightly better than free DTX/GEM. According to the results obtained, FA-PEG2000-PLGA-DTX/GEM NPs treatment reduced cell survival more significantly compared with other groups.

Figure 4 The cytotoxicity assay of different DTX/GEM formulations in SKOV-3 cells. *p<0.05, The other three groups vs FA-PEG2000-PLGA-DTX/GEM NPs. (n=6).Abbreviations: DTX, docetaxel; GEM, gemcitabine; NPs, nanoparticles.

The half-maximal inhibitory concentration (IC50) values were measured by GraphPad Prism 5. Briefly, the IC50 value of the FA-PEG2000-PLGA-DTX/GEM NPs was 175.3 ng/ml compared with a value of 629.4 ng/ml for free DTX/GEM and 437.1 ng/ml for the nontargeted NPs, PLGA-DTX/GEM NPs.

Cellular uptake

Confocal microscopy was employed to characterize the cellular internalization of free coumarin 6, coumarin 6-loaded PLGA NPs and coumarin 6-loaded FA-PEG2000-PLGA NPs in SKOV-3 cells. As shown in Figure 5A, a strong green fluorescence was observed in the cytoplasmic region of coumarin 6-loaded FA-PEG2000-PLGA NPs after incubation for 2 h. In SKOV-3 cells, the cell internalization of coumarin 6-loaded FA-PEG2000-PLGA NPs was higher than others.

Figure 5 (A) Fluorescence microscope images showing the cellular uptake in SKOV-3 cells. (A) free DTX/GEM; (B) PLGA-DTX/GEM NPs; (C) FA-PEG2000-PLGA-DTX/GEM NPs. Magnification ×200. (B) Mean fluorescence intensity percentage as determined by flow cytometry experiments. *p<0.05, The other three groups vs FA-PEG2000-PLGA-DTX/GEM NPs. (n=6).Abbreviations: DTX, docetaxel; GEM, gemcitabine; NPs, nanoparticles.

The results showed that more drugs entered the cell successfully through surface modification of NPs. In the study of quantitative cell uptake, through the recovery of drug NPs from the celland coumarin-6 in the three formulations was quantified by measuring the fluorescence. Quantitative results were consistent with the confocal images (Figure 5B).

Antitumor study

To determine the therapeutic effects of the FA-PEG2000-PLGA-DTX/GEM NPs, nude mice were implanted with SKOV-3 cells and used for therapeutic efficacy evaluation of free DTX/GEM or DTX/GEM NPs. As shown in Figure 6, the other three groups were better able to inhibit tumor growth and decrease tumor size when compared with the control group. At the end of the experiment, tumors were found to be more sensitive to FA-PEG2000-PLGA-DTX/GEM NPs at a volume of 243.6±123.9 mm3, compared to that of the PBS group (1237.2±276.3 mm3); free DTX/GEM (787.6±131.7 mm3) and PLGA-DTX/GEM NPs (512.5±156.1 mm3). The results indicated a significant effect on the reduction rate of tumor volume for FA-PEG2000-PLGA-DTX/GEM NPs, which was statistically higher than other groups. Because of the EPR effect and receptor ligand-mediated targeted delivery, FA-modified NPs increased accumulation of DTX/GEM in tumors and enhanced the anticancer effect in vivo (Figure 6A).19,20 Toxicity of normal organs has always been a common limiting factor in clinical use of anticancer drugs. Thus, the body weight and survival rate of mice were tested to evaluate the toxicity of NPs. There was no significant change in body weight and organ coefficient in different preparations (Figure 6B). In addition, no death occurred until the completion of this study. It was confirmed in vitro that NPs allowed sustained release of the entrapped drug, which might result in a decrease in the dose administered and in toxic side effects. H-E staining was used to determine whether there was organ damage. The results showed that there were no obvious lesions in heart, liver, spleen, lung or kidney of the four groups, suggesting that DTX/GEM had no obvious acute toxicity in vivo (Figure 6C).

Figure 6 The antitumor activity of free DTX/GEM; PLGA-DTX/GEM NPs and FA-PEG2000-PLGA-DTX/GEM NPs on SKOV-3 tumor-bearing nude mice. (A) Tumor volume growth curves. (B) Body weight variations of mice during the treatment. (C) Histopathological studies of heart, liver, spleen, lung and kidney. *p<0.05, The other three groups vs FA-PEG2000-PLGA-DTX/GEM NPs. (n=6). Abbreviations: DTX, docetaxel; GEM, gemcitabine; NPs, nanoparticles.


In this study, a novel FA-targeted co-delivery of DTX and GEM NPs was developed to overcome ovarian cancer. The hemolysis results indicated that FA-PEG2000-PLGA was potentially feasible for targeted antitumor drug delivery through blood circulation. In vitro release study suggested that in comparison with the free drug, PLGA-DTX/GEM NPs and FA-PEG2000-PLGA-DTX/GEM NPs had better sustained-release properties. However, there was no obvious difference between the two NPs with the same drug in the release profile. Ovarian cancer cells in vitro efficiently took up the non-targeted and FA-targeted NPs; improved cytotoxicity was observed for the FA-targeted NPs, showing a 3.59- fold drop in the IC50 in SKOV-3 cells as compared to DTX/GEM alone. Cellular uptake showed that because of surface modification, more drugs entered the cell successfully. Pharmacodynamics results showed a significant effect on the reduction rate of tumor volume for FA-PEG2000-PLGA-DTX/GEM NPs, which was statistically higher than other groups, and no toxicity of organs.


This study was funded by National Natural Science Foundation (81572546, 81372305), the project of Tongji University basic research capability enhancement (20120170047).


The authors report no conflicts of interest in this work.


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