Anti-platelet effects of anti-glaucomatous eye drops: an in vitro study on human platelets
Received 4 January 2017
Accepted for publication 13 March 2017
Published 19 April 2017 Volume 2017:11 Pages 1267—1272
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 4
Editor who approved publication: Dr Tuo Deng
Marilita M Moschos,1,2 Giannis A Moustafa,1 Vasiliki D Papakonstantinou,3 Michael Tsatsos,4 Konstantinos Laios,1 Smaragdi Antonopoulou3
11st Department of Ophthalmology, Medical School, University of Athens, Athens, Greece; 2Biomedical Research Foundation, Academy of Athens, Athens, Greece; 3Laboratory of Biology, Biochemistry, Physiology and Microbiology, Department of Nutrition and Dietetics, School of Health Science and Education, Harokopio University, Athens, Greece; 4Royal Eye Infirmary, Dorset County Hospital NHS Foundation Trust, Dorchester, UK
Purpose: Altered platelet aggregability has been implicated in the pathogenesis of glaucoma. This study aims to investigate the anti-platelet potential of intraocular pressure lowering drops, with the possibility of establishing it as an additional mechanism of anti-glaucomatous action.
Materials and methods: The anti-aggregating effects of a series of anti-glaucomatous eye drops were determined on human platelets in the platelet aggregation model, using four known aggregating factors (platelet activating factor [PAF], adenosine diphosphate [ADP], thrombin receptor-activating peptide [TRAP], and arachidonic acid [AA]).
Results: Almost all of the tested samples inhibited platelet aggregation induced by PAF, ADP, TRAP, and AA, except for Alphagan, which did not demonstrate inhibition of ADP- and TRAP-induced aggregation at a wide range of concentrations. Trusopt, Betoptic, and Azarga eye drops were the most potent inhibitors of all four aggregating factors, while Alphagan was the least potent (P<0.05).
Conclusion: This study shows that anti-glaucomatous eye drops possess anti-platelet effects, and this was shown for the first time by experimenting on human platelets.
Keywords: glaucoma, eye, platelet, aggregation, PAF, TRAP
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