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A Response to Article “Anti-Diarrheal Activity of Hydromethanolic Crude Extract and Solvent Fractions of Acacia Seyal (Fabaceae) Roots in Mice” [Letter]
Authors Panjaitan NSD , Fitriana F, Handayani S
Received 18 November 2022
Accepted for publication 25 November 2022
Published 29 November 2022 Volume 2022:14 Pages 111—112
DOI https://doi.org/10.2147/CPAA.S398217
Checked for plagiarism Yes
Editor who approved publication: Professor Arthur E. Frankel
Novaria Sari Dewi Panjaitan, Fitriana Fitriana, Sarwo Handayani
Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency (BRIN), Cibinong Science Center, Cibinong, West Java, Indonesia
Correspondence: Novaria Sari Dewi Panjaitan, Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency (BRIN), Genomic Building, Cibinong Science Center, Jl. Raya Bogor No. 490, Km. 46, Cibinong, West Java, Indonesia, Email [email protected]
View the original paper by Mr Mengesha and colleagues
A Response to Letter has been published for this article.
Dear editor
We were interested in reviewing the study performed and reported by Mengesha et al, regarding the evaluation of anti-diarrheal activity of the extract of Acacia seyal root tested in mice.1 Interestingly, according to our searches, there were still very limited studies regarding Acacia seyal and most of those utilized its gums and bark for its antioxidant, antibacterial, cytotoxic till antimammary activities of Acacia seyal.2–4 This study was likely to be the first evaluation study of the anti-diarrheal activity of the hydromethanolic extract of Acacia seyal, especially and specifically utilizing its root. Following the traditional method of organic solvent extraction, the hydromethanolic crude extract of Acacia seyal root was also separated in aqueous, n-hexane and ethyl acetate fractions.
Based on the results obtained and reported in the article, all fractions of Acacia seyal root hydromethanolic crude extract and the fractions separated in aqueous, n-hexane, ethyl acetate fractions, showed anti-diarrheal activity in dose-dependent manner. The single component detection in all fractions and crude extract showed the presence of similar metabolites containing steroids, phenols, anthraquinones, and glycosides. However, the specific levels or concentrations of those metabolites were not detected specifically in each fraction. Therefore, we recommend the detection of specific levels of each secondary metabolite in each fraction in future studies. It was also unclear whether those metabolites are dependent of each other or could be utilized as anti-diarrheal agent in single purified metabolite.
The study model used in this study was diarrheal model induced by oil-castor. This study model is a traditional model which had been used in studies for decades. However, under the physiological condition, diarrhea is frequently caused by the infections.5 Therefore, the bacteria infection model could be taken as consideration for being used for the follow-up of this study in the near future.6 Overall, this study still convey the novel idea of exploring the anti-diarrheal activity possessed by the root extract of Acacia seyal which hopefully could be further explored and studied for addressing the suggestions provided above.
Acknowledgments
We would like to appreciate all supports given during the study performed by Mengesha, A. K. et al, and their article preparation. In addition, appreciations should be given to Dr Sunarno for his continuous support for the authors of this letter to the editor.
Author Contributions
NSDP, FF and SH read and reviewed the article. NSDP conceived the critical design of the letter. NSDP went through the cited publications and analyzed the reported data. NSDP wrote the whole letter draft. FF and SH recommended required corrections. NSDP, FF and SH revised the letter manuscript accordingly.
Disclosure
All authors clearly stated that there is no conflict of interest in this communication.
References
1. Mengesha AK, Birru EM, Adugna M. Anti-diarrheal activities of hydromethanolic crude extract and solvent fractions of acacia seyal (Fabaceae) roots in mice. Clin Pharmacol. 2022;14:99–110.
2. Elmi A, Spina R, Risler A, et al. Extracts and isolated compounds. Molecules. 2020;25(2392):1–15.
3. Ahmed AA, Fedail JS, Musa HH, Kamboh AA, Sifaldin AZ, Musa TH. Gum Arabic extracts protect against hepatic oxidative stress in alloxan induced diabetes in rats. Pathophysiology. 2015;22(4):189–194. doi:10.1016/j.pathophys.2015.08.002
4. Zingue S, Njuh AN, Tueche AB, et al. In vitro cytotoxicity and in vivo antimammary tumor effects of the hydroethanolic extract of acacia seyal (Mimosaceae) stem bark. Biomed Res Int. 2018;2018:1–13. doi:10.1155/2018/2024602
5. Alharbi MG, Al-hindi RR, Esmael A, et al. The “big six”: hidden emerging foodborne bacterial pathogens. Trop Med Infect Dis. 2022;7:1–19.
6. Wu Z, Xu Q, Li A, Lv L, Li L. Apple polyphenol extract suppresses clostridioides difficile infection in a mouse model. Metabolites. 2022;12:1042.
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