A Novel Silicon Platform for Selective Isolation, Quantification, and Molecular Analysis of Small Extracellular Vesicles
Received 12 March 2021
Accepted for publication 20 May 2021
Published 28 September 2021 Volume 2021:16 Pages 5153—5165
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Professor Israel (Rudi) Rubinstein
Antonio Alessio Leonardi,1– 3 Rosalia Battaglia,4 Dario Morganti,1,2 Maria Josè Lo Faro,1,3 Barbara Fazio,2 Chiara De Pascali,5 Luca Francioso,5 Gerardo Palazzo,6,7 Antonia Mallardi,8 Michele Purrello,4 Francesco Priolo,1 Paolo Musumeci,1,* Cinzia Di Pietro,4,* Alessia Irrera2,*
1Dipartimento di Fisica e Astronomia, Università di Catania, Catania, 95123, Italy; 2CNR-IPCF, Istituto per i Processi Chimico-Fisici, Messina, 98158, Italy; 3CNR-IMM UoS Catania, Istituto per la Microelettronica e Microsistemi, Catania, 95123, Italy; 4Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy; 5CNR-IMM, Institute for Microelectronics and Microsystems, Via Monteroni, University Campus, Lecce, 73100, Italy; 6Chemistry Department, University of Bari ‘Aldo Moro’, Bari, 70125, Italy; 7CSGI, Center for Colloid and Surface Science c/o Chemistry Department, Bari, 70125, Italy; 8CNR-IPCF, Institute for Chemical-Physical Processes, c/o Chemistry Department, Bari, 70125, Italy
*These authors contributed equally to this work
Correspondence: Paolo Musumeci; Cinzia Di Pietro Email [email protected]; [email protected]
Introduction: Small extracellular vesicles (sEVs), thanks to their cargo, are involved in cellular communication and play important roles in cell proliferation, growth, differentiation, apoptosis, stemness and embryo development. Their contribution to human pathology has been widely demonstrated and they are emerging as strategic biomarkers of cancer, neurodegenerative and cardiovascular diseases, and as potential targets for therapeutic intervention. However, the use of sEVs for medical applications is still limited due to the selectivity and sensitivity limits of the commonly applied approaches.
Methods: Novel sensing solutions based on nanomaterials are arising as strategic tools able to surpass traditional sensor limits. Among these, Si nanowires (Si NWs), realized with cost-effective industrially compatible metal-assisted chemical etching, are perfect candidates for sEV detection.
Results: In this paper, the realization of a selective sensor able to isolate, concentrate and quantify specific vesicle populations, from minimal volumes of biofluid, is presented. In particular, this Si NW platform has a detection limit of about 2× 105 sEVs/mL and was tested with follicular fluid and blastocoel samples. Moreover, the possibility to detach the selectively isolated sEVs allowing further analyses with other approaches was demonstrated by SEM analysis and several PCRs performed on the RNA content of the detached sEVs.
Discussion: This platform overcomes the limit of detection of traditional methods and, most importantly, preserves the biological content of sEVs, opening the route toward a reliable liquid biopsy analysis.
Keywords: silicon nanowires, biosensor, small extracellular vesicles, label-free, luminescence
Various biomarkers have been discovered and characterized as novel tools to quickly and easily monitor a patient’s health state related to different diseases.1–5 Among these, extracellular vesicles (EVs) and their content have taken on an increasingly important role in the last few years. EVs, commonly identified as a heterogeneous membrane vesicle population6–9 including large EVs (lEVs) and small EVs (sEVs) carry proteins, lipids, RNA, and DNA, from one cell to another.10–12 The analysis of their cargo has found different applications in precision medicine providing diagnostic, prognostic, and/or therapeutic information. In oncology research and diagnosis,10,13,14 liquid biopsy represents the most promising application. The isolation and purification of sEVs from biological fluids, (serum or plasma) and the molecular analysis of their cargo offer the possibility of monitoring tumor state without the use of invasive surgical procedures. The liquid biopsy strategy has also been proposed for other diseases such as cardiovascular diseases15 or atherosclerosis.16 Moreover, in In vitro Fertilization (IVF) procedures sEV analysis may be able to predict the success of a pregnancy, replacing the more invasive pre-implantation genetic diagnosis technologies.17 Despite remarkable progress in EV research, the complexity and challenges associated with the lack of standard methods to accurately isolate, characterize and quantify them remain considerable.18
The most widely used techniques to isolate sEVs (ultracentrifugation, density gradients, precipitation, filtration, size exclusion chromatography, and immunoisolation) have many drawbacks including the co-isolation of EV subtypes, sample contamination with protein aggregates, low RNA yield, and the modification of the biophysical properties of the isolated vesicles.18–20 In the last few years, new methods able to distinguish EV subpopulations from EV isolates have been proposed. Capillary electrophoresis (CE) that separates analytes in a capillary tube under the influence of an electric field according to their different charge-to-hydrodynamic radius ratios, has recently been described for the characterization of EV preparations with ultraviolet (UV) or fluorescence detection.21–23 In 2019, Zhang and Lyden optimized asymmetric-flow field-flow fractionation technology for separating and characterizing extracellular nanoparticles, particularly exosomes.24
Moreover, these procedures require bulky and expensive instrumentation, time-consuming and laborious processing, and large amounts of the starting samples.28 Considering that their concentration in biological fluids depend on the number of cells involved in the secretion and up-take as well as changes in different physiological or pathological conditions (infectious diseases, cellular senescence, age-related neurodegenerative diseases, and cancer), the concentration may be very low to make impossible their isolation and an accurate quantification. For example, in the fluid produced by the embryo during the early stages of preimplantation development (blastocoel fluid) their concentrations are so low that it is not possible to isolate and measure them with the techniques available today.17
An interesting approach is micro-Nuclear Magnetic Resonance (µNMR), based on the labeling of sEVs through magnetic beads (diameter around 500 nm) that are read in a microfluidic chip after filtering larger vesicles.25 Despite the remarkable limit of detection (LOD) of 107 Ex/mL this sensor requires a microfluidic approach with high costs that limit its application.
The innovative properties of nanomaterials, as well as their increased high surface to volume ratio, make nanotechnology a strategic tool to overcome standard sensor limits.26–28 Surface Plasmon Resonance (SPR) is based on the refractive index change upon the target binding to the transduction sensor interface.29–33 A nanoplasmonic system called nPLEX has a LOD 100 times better than ELISA (whose LOD is around 108 EX/mL) for sEV detection.34 A drawback is the high cost of the microelectronics-grade fabrication requirement of this platform, which makes a capillary diffusion of these sensors difficult.34
Sensors do not couple selectivity, high sensitivity, and small volume analysis that are indeed considered strategic for several EV applications and they are commonly characterized by a high production cost and the incompatibility of their synthesis approaches with current industrial technology.
A silicon-based platform is of absolute interest for real marketable applications and 1D materials such as Si NW are emerging as an integrable solution with the standard technology offering a robust platform with very a high aspect ratio and exposed surface.35–40 Si NW-based sensors are commonly based on the electrical signal variation in field effect transistor configuration with a very high cost of fabrication and electrical addressing due to the lithographic processes required.41,42
Recently, we have demonstrated the realization of room temperature (RT) luminescent Si NWs by a cost-effective, fast, and industrially compatible approach43–45 that opens the route to a novel class of sensors. Si NW label-free luminescent sensors have demonstrated high selectivity guaranteed by proper functionalization processes and high sensitivity able to surpass standard bulk approaches such as ELISA by several orders of magnitude,46,47 providing a robust and very appealing platform for real commercial transfer.
Our proposed Si NW luminescent sensing system can be functionalized with a specific antibody able to selectively bind the vesicles that present the respective antigen. In this work, we were able to isolate, concentrate, and quantify sEV CD81+ with a sensitivity higher than the actual nanostructured sensors, with a key working range, expressed as sEVs/mL (Ex/mL) concentration, which is found in most bio-fluid analyses. The high sensitivity allowed us to isolate sEV CD81+ and estimate their concentration in a very small volume of blastocoel fluid from a single embryo. Moreover, we demonstrated that it is possible to recover sEVs, and that the isolation, quantification, and detachment procedures do not cause nucleic acid degradation. We believe that our platform, being able to be functionalized with different antibodies, opens very interesting perspectives for the future application of liquid biopsy in personalized medicine and in preimplantation genetic analysis.
Si NW Fabrication
The fabrication of circular Si NW cavities was carried out, obtaining a working area with an accurately determined size, and we performed the measurements on a well-defined functionalized area with a highly reproducible protocol.
Exposed Si circular cavities of 100-400 µm in diameter were obtained by a photolithographic process (see Supplementary Information, section 1). The formation of Si NWs is driven by the use of a percolative metal film deposited by an electron beam evaporator and, thanks to the photolithographic mask, the metal deposition was obtained only in the target area, ie, the designed circular spot. Thus, Si NWs were realized only inside these cavities, as reported in the experimental section. This approach also enables the fabrication of multi-probe arrays, considering that the fabricated circular cavities can serve as an embedded microfluidic platform for very precise sample confinement on the chip.
In Figure 1A and B, the SEM images of the synthetized Si NWs in the circular cavities are shown, in plan view and in cross section, respectively. In the inset of Figure 1A a magnification of one of the borders is shown, which shows the Si NW morphology, highlighting the high uniformity and the defined development of these structures. The cross section shown in Figure 1B clearly shows the profile of the cavity border where it is possible to see that the Si NW growth on that region (about 2 µm wide) is affected by the photoresist mask wall-effect on solution diffusive processes. Outside this small region (about 2% of the cavity diameter), Si NWs show a narrowly distributed length of 3.3 µm.
Figure 1 (A) Plan view SEM image of the Si NW cavity designed. In the inset the border part attesting the good features of the Si NWs is reported. (B) cross section SEM image of the Si NWs showing the good quality also near the border.
An ad-hoc protocol of functionalization of the silicon nanowires was carried out to make the sensor highly selective for the detection of the CD81+ sEVs towards other vesicles. This protocol is based on the know-how previously developed by our group in the realization of Si NWs-based sensors46,47 and it is shown in Figure 2A–D (more details in the experimental section). Finally, the obtained sensing platform was tested with standard solutions at different concentrations of sEVs (Figure 2D).
A confocal microscopy analysis was carried out to demonstrate the functionalization protocol efficacy and the ability of the Si NW sensor to capture target sEVs. In Figure 2E–H the micrographs obtained by laser scanning confocal microscopy (LSCM) of the vertical sections (X-Z) of the Si NW layer are shown. The appropriate choice of the excitation and emission wavelengths (see Supplementary Information) discriminates the signal of just one type of marked molecule at a time. In this way, it is possible to analyze the correct functionalization of each step of the sequential binding of streptavidin, anti-CD81 and the capture of EVs.
In Figure 2E the signal obtained from the Si NWs is shown. The success of the functionalization is attested by the analysis shown in Figure 2F and G showing the streptavidin and the biotinylated anti-CD81 signals, respectively. These signals coincide with the Si NW one demonstrating functionalization success.
Anti-CD81 can selectively bind to the corresponding tetraspanin present on the surface of sEVs, thus anchoring the extracellular vesicles to the silicon NWs (as shown in Figure 2D). The anchoring of the sEVs can also be probed by LSCM. For this purpose, the sEVs had been previously labelled with a phosphoethanolamine bearing the fluorophore NBD. Figure 2H shows the successful capture of the sEVs after the incubation of the sensor with the labelled sEVs, notwithstanding extensive washing.
After proving the effectiveness of the functionalization, a negative test was designed to demonstrate the selectivity of the Si NW platform.
Figure 3A shows PL spectra, carried out at room temperature, of the as-synthetized Si NWs (black curve), as well as of the Si NWs after immersion in a PBS solution in presence or not of synthetic vesicles (VES, 100 nm diameter) used as an EV mimetic system. The figure shows that the presence of the PBS matrix causes a small quenching of the Si NW PL signal. However, a variation below 4% was detected in the presence of synthetic VES with a concentration of 3×1011 VES/mL (red curve) demonstrating that the Si NW sensing platform does not respond to other types of vesicles, thus proving the high selectivity of the sensor.
The platform performance was tested with an sEV concentration range from 107 to 1011 sEVs/mL (Ex/mL) and the obtained PL spectra at room temperature are shown in Figure 3B. The signal of the sensor immersed in PBS without sEVs is shown in black and is considered the sensor reference signal. Increasing the concentration of the sEVs, a corresponding increase of the sensor PL quenching that is used as a sensing mechanism is clearly visible. Indeed, the calibration curve was obtained by plotting the quenching of the platform versus the concentration of the sEVs. The PL signals were obtained by fitting the Gaussian area of each spectrum and normalizing it to the area of the reference one: thus, the quenching value was obtained as the complementary part (the missing part) of the PL signal with respect to the reference one. In this way, the higher the sensor signal obtained, the higher the sEVs corresponding concentration. As shown in Figure 3C, this allowed us to fit the dose response curve with Hill’s binding model,46,48 commonly used to describe the performance of an antibody/antigen reaction. Further details on the model can be found within the Suppl. Information.
As shown in Figure 3C, a 35% Si NW PL quenching corresponds to a 107 Ex/mL concentration, while a further 26% PL quenching is observed reaching a 108 Ex/mL concentration. Between 108 and 1010 Ex/mL, the response of the sensor approaches a linear trend with a 10% variation for each increase of one order of magnitude of the sEVs concentration. Finally, from 1010 Ex/mL to 1011 Ex/mL, a small further quenching (an increase of just 4%) suggested that the sensor saturation was reached. The fitted calibration curve shows a LOD, estimated considering the concentration that corresponds at 2 times the average error on the PL response with respect to the minimum asymptotic value of the calibration curve, in the order of 2×105 Ex/mL. This value surpasses the sEVs LOD obtained through bulk methods and nanostructures in the literature by at least 2 orders of magnitude. The adopted functionalization has already been successfully used for CRP analysis.49 However, to prove the robustness of this result, all the reported PL measurements were performed on several sensors, always obtaining the same result within a few percentage points of relative error (as reported in the spectra in Figure 3).
Subsequently, the Si NW sensor was tested with some real samples, previously investigated with other standard approaches. In particular, the Si NW sensor was tested with sEVs purified from two follicular fluid (FF) samples (FF-EVs 1, FF-EVs 2) and with a sample of blastocoel fluid (BF) from a single embryo. Further details on these samples can be found in the Supplementary Information. The PL quenching obtained with these real samples was used to find the indirect concentration, thanks to the calibration curve obtained for the Si NW sensor. In Figure 3D, the measured PL quenching for FF and BF reported on the calibration curve shows the sEVs concentration measured by the Si NW sensor. In particular, for the FF-EVs 1 and FF-EVs 2 samples (for two samples of human FF), whose concentrations measured with the nanoparticle tracking analysis (NTA) were about 1–2×1011 Ex/mL, concentrations of about 1 and 6×109 Ex/mL were found, respectively. The difference between the values obtained through an immunoassay (as our Si NW sensor) and a non-selective method such as nanoparticle tracking analysis is quite common. Indeed, non-specific methods cannot distinguish sEVs among the other types of vesicles and thus the measured concentrations were higher than those obtained using a selective sensor as also observed comparing ELISA and NTA in our previous study.17
Furthermore, our Si NW sensor was tested with one blastocoel fluid sample diluted in about 50 µL of H2O. The obtained result, indicated as blastocoel* in Figure 3D, shows a concentration of 2.6×106 Ex/mL that corresponds to 1.6×107 Ex/mL considering the added solvent (50 µL). The small amounts of volumes available for the measurement is a strong practical limit of the traditional approaches, whereas these samples are easily analyzable by this Si NW platform. In fact, a BF sample is commonly limited to a volume of 0.3–0.5nL (that are dissolved in a few microliters of H2O during sample withdrawal)17 and thus the low sEV concentrations are not measurable in a sample from a single embryo through NTA or ELISA. The measurements are commonly carried out in a pool of several samples mixed together and to evaluate embryo competence in IVF cycles it is necessary to carry out the analysis on the individual embryo.17 The measurements performed on a pool of three different samples for NTA and a pool of six samples for ELISA, indicate a concentration of 2.46×109 Ex/mL by NTA and 3.3×108 Ex/mL by ELISA17 by using anti-CD63. Moreover, in this case a strong difference (one order of magnitude) was found between selective and non-selective approaches.
EV Recovery and Molecular Analysis of the Cargo
Another challenge of strategic relevance is the selective isolation of sEVs for further studies. In fact, our sensor is able, after sEV quantification, to detach the selectively isolated sEVs, allowing a further analysis with other approaches (the protocol used for detaching EVs is reported in the Supplementary Material). To demonstrate this possibility, we investigated the sEVs selectively isolated from our Si NW platform and detached with a proper protocol by SEM analysis (bio-sample treatment in Supplementary Information). In Figure 4A a plan view SEM image obtained showing a distribution of sEVs is shown. We performed a statistical analysis on the SEM image of the detached sEVs finding a Gaussian distribution with an average size of 94 ± 32 nm and an imperfect agreement with the previous NTA measurements and typical for an sEV and exosome distribution.17 As shown in the inset of Figure 4A, the morphology of the sEVs is preserved and is comparable before and after treatment.
Finally, to demonstrate the possibility to perform molecular analysis on the EV cargo, we focused our attention on RNA molecules (miRNA and lncRNA) because they represent the most promising biomarkers. We verified that the optical analysis, by laser measurements, did not influence RNA quality and also that PBS or Qiazol can be used for the storage of NWs after EV capture (details and negative tests in the Supplementary Materials). Figure 4B shows the results of Real Time Polymerase Chain Reaction (RT-PCR) for miR-212 reported as 2−Ct values on two follicular fluid samples, indicated as FF-EVs 3 and 4.
Finally, we demonstrated the integrity of RNA cargo inside the EVs isolated by our sensor, both for small RNAs (miRNA) and for long RNAs (lncRNA). We analyzed miR-132, miR-203 and MALAT1 in scalar dilutions of the same samples, by Real-Time RT-PCR. We found a significant correlation between the Ct values (represented as 2−Ct) and the three dilutions for both miRNAs and MALAT1 (Figures 5 and 6).
The characterization and analysis of DNA and incRNAs enclosed inside sEVs and released from tumor cells into biological fluids is perhaps the most promising application of liquid biopsy. In fact, in a minimally invasive way, it is possible to obtain diagnostic, prognostic, and predictive biomarkers to use in precision medicine. Liquid biopsy can be applied not only in cancers but also in different pathologies such as neurodegenerative diseases and in preimplantation diagnosis.10,13,14 Despite remarkable progress, the complexity and challenges of standard methods to accurately isolate, characterize, and quantify EVs represent limiting factors in the application of the procedure in clinical practice.18–20
In this paper, a Si-based platform realized with a low-cost industrially compatible process is reported for the first time as a novel selective platform. This sensor surpasses traditional approaches in the isolation, concentration, and quantification of sEVs. Indeed, this Si NW sensor shows a limit of detection of about 2×105 Ex/mL and the possibility to carry out the measurement on very small volume samples, surpassing traditional approaches that require volumes of at least 100 µL and are, at the same time, far less sensitive. To the best of our knowledge this sensor surpasses the state of the art LOD of the other sensors reported in the literature of at least one order of magnitude.34 Compared with standard immunoassays that present some of the best LODs such as ELISA (whose LOD is around 108 EX/mL) the advantage is of several orders of magnitude for sEV detection. Moreover, the operating range of this Si NW platform between 105 and 1011 sEVs/mL is perfectly tailored to work with real sEV concentrations in human bio-fluid samples.
We tested the sensitivity of our platform with blastocoel fluids (BF) representing a case in point where standard approaches usually fail. Indeed, to characterize BF sEVs by NTA at least three different samples have to be pooled and the obtained values are close to the LOD for both NTA tracking analysis and ELISA, making their measures complex and sometimes unreliable.17 However, as shown in this paper, by using this novel Si NW platform we were able to isolate and quantify BF sEV CD81+ from a single sample, addressing the crucial demand for higher performance sensors (Figure 3D). This Si NW sensor can perfectly operate with these small volumes and concentrations that cannot be measured by other standard approaches such as dynamic light scattering, NTA, and ELISA. Moreover, by using a microliter dispenser and thanks to the photolithography process cavity-based approach, this sensor could be used with even smaller volumes.
Another remarkable point is the possibility to recover the isolated and quantified vesicles to directly carry out the molecular analysis of the cargo on the same aliquot. Furthermore, we demonstrated that the isolation and quantification steps preserve vesicle morphology and do not degrade nucleic acid cargo (Figures 4–6). In fact, in scalar dilutions of the same sample, loaded onto three different Si NW platforms, a significant correlation between nucleic acid concentration and the dilutions was found. We verified that not only the miRNAs, well-known to resist degradation, but also MALAT1, a long RNA molecule, can be properly analyzed after the isolation and quantification by our sensor (Figures 5 and 6).
In this work, CD81+ sEVs have been isolated but it could be possible to isolate other specific vesicle subpopulations by using different specific antibodies and to release them unmodified, even after the quantification process. Thus, this device offers the possibility to study only the content of the selected sEVs among the other EVs present in bio-fluids. The future perspectives of this novel platform are manifold, not all for the quantification but above all for the further study of macromolecules such as DNA or RNAs inside sEVs with a tremendous impact on both sEV research and precision medicine.
Commercial 4” Si wafers were purchased from Siegert Wafer, whereas hydrofluoric acid (HF) and hydrogen peroxide were from Honeywell and Sigma Aldrich, respectively. Streptavidin and Phosphate Buffer Saline (PBS, 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4, at 25 °C) tablets were obtained from Sigma Aldrich, while the biotinylated anti-CD81 monoclonal antibody (speciﬁc for CD81 binding) was purchased from LifeSpan BioSciences. Exosomes were acquired from Hansa BioMed Life Sciences Ltd. Milli-Q water (18 MOhm × cm) was used in all steps of the synthesis and for the preparation of the aqueous solutions used for the functionalization.
Si NW Fabrication
The whole manufacturing procedure for the Si NW cavity fabrication is reported in the Supplementary Information. After a standard microelectronics-grade silicon cleaning with acetone and 2-propanol and Milli-Q water, followed by a hotplate de-hydration at 120°C for 600 sec, a negative resist was dispensed obtaining a layer with a final thickness of about 1.4 µm (AZ5214). The patterning of the photoresist was realized by a 365 nm optical lithography process in hard contact mode with a Karl Suss MA6 tool. The final circular patterns used for this activity had a diameter of about 100 µm. After the developing procedure, in order to increase the mask etching selectivity by the HF bath for the nanowire formation, a hard bake step at 150 °C for 10 min was performed on a hotplate.
Silicon nanowires were fabricated through a modified metal assisted chemical etching that involved the use of a thin discontinuous gold film.45 A 2 nm gold layer was deposited by an Electron Beam Evaporator (Kenosystec equipment) at ultrahigh vacuum and at room temperature. The samples were then immersed in an aqueous solution of HF (2.5M) and H2O2 (0.25M). Gold acts as a catalyst and, in the presence of H2O2, as an oxidizing agent, driving the oxidation of the Si that is in contact with the metal layer. Hydrofluoric acid etches the silicon oxide driving the anisotropic etching of the Si just underneath this metal mask. Uncovered silicon regions remain unattached and form the Si NWs. As the last step, a gold etchant was used to completely remove the gold metal layer. All the chemical processes were carried out at room temperature and no gold contamination was detected inside the wires.
After the fabrication procedure, the Si NW arrays were cleaned by immersion in isopropanol (IPA) 1m, followed by 1min in Milli-Q water to remove the IPA residue, and finally dried by in a nitrogen flux. The functionalization protocol50 was divided into 3 steps: i) Sample immersion in a streptavidin 10 µg/mL solution overnight (about 14 h); ii) Sample immersion in a biotinylated Anti-CD81 (Ab) 50 µg/mL solution for 4 h; iii) Immersion in the exosome solution overnight.
After each step, the sensing platform was rinsed in 10 mM phosphate buffer saline (PBS) three times to remove unconjugated molecules. Streptavidin was deliberately used as this protein strongly binds biotin. The dissociation constant for streptavidin-biotin binding is Kdiss= 10−15 M−1, allowing the efficient attachment of the biotinylated antibody for CD81, which is a transmembrane protein expressed on the phospholipidic membrane of sEVs. Thus, the use of the specific antibody guarantees the high selectivity required by this type of application. All the functionalization solutions and the exosome solutions used for the calibration were prepared using PBS.
The protocol adopted for the dye-binding used in the confocal microscopy experiment is reported in the Supplementary Information.
EV Isolation and Quantification
Follicular Fluid (FF) of preovulatory follicles was collected from women undergoing intracytoplasmic sperm injection (ICSI) cycles, recruited at the IVF Unit Cannizzaro Hospital, Catania (Italy), according to a previously published protocol.51 EVs from FF samples were isolated by differential ultracentrifugation according to the protocol by Santonocito et al with minor modifications and resuspended in 1 mL PBS.51,200 μL of EV suspension was loaded onto Si NWs for EV quantification.
To test the sensitivity of the Si NW array we quantified EVs also in BF samples from a single embryo. Expanded blastocysts on day 5 underwent blastocentesis and cryopreservation as previously described.17 Around 0.3–0.5 nL of BF isolated from each blastocyst were transferred to a PCR tube with 5 μL of RNase-free water and stored at −80 °C until further processing. Because of the low quantity of samples, no procedure of EV purification was performed. Specifically, BF samples diluted in 50 μL PBS were loaded onto Si NWs for EV isolation and quantification.
Structural and Optical Measurements
The scanning electron microscope (SEM) images were acquired using a ZEISS Supra 25 microscope by an InLens detector with typically 5 KeV of extraction voltage.
In the case of the exosome images, a typical protocol for the analysis of biological samples was used (more detail in the Supplementary Material).17 The solution containing the exosomes detached from the Si NW platform was drop-cast onto a glass support on the top of a SEM sample holder and was then dehydrated by several washing steps with aqueous solutions at increasing IPA percentages. Finally, as widely reported in the literature, the sample was decorated with gold through a previously calibrated sputtering procedure obtaining a uniform gold decoration. This step is essential to make the sample conductive as required by a standard SEM analysis.
Laser scanning confocal microscopy (LSCM) was carried out with a direct confocal microscope (Leica TSC SP8 TCS SMD FLCS, Leica Microsystem, Germany) by using a fully tunable supercontinuum white-light laser. To avoid perturbations associated with the use of an immersion objective, a low-resolution 20x dry objective was used. Therefore, the size of the analyzed structures is exaggerated. All data were subsequently processed with Leica LAS AF LITE (Leica Microsystems, Germany).
The photoluminescence signals were acquired using a HR800 spectrometer by Horiba Jobin-Yvon. The excitation was obtained through the 476.5 nm line of an Ar+ laser and focused through a 100X (NA=0.9) objective with a power of 100 µW onto the sample plane. The Spectrometer works in a backscattering configuration and the signal is then obtained by the same objective, spectrally decomposed by a 600 L/mm grating, and acquired through a Peltier cooled CCD at −70°C (Synapse).
EV Recovery and Molecular Analysis of the Cargo
To verify the possibility of recovering undamaged EVs after isolation and quantification by Si NWs, the platform with sEVs from BF was transferred into a new 1.5 mL Eppendorf tube containing PBS 1x (diluted 1:5 in RNase-free water) and heated to 60 °C for 60 sec and then removed.52 EV elution was then transferred at −20 °C and processed for SEM analysis.
To verify the best way to store and later analyze the vesicle content demonstrating that the laser treatment does not affect RNA integrity and amplification, we assayed miR-212 in different samples. Specifically, Si NWs with or without laser exposure and differently stored, with either 200 μL of PBS or Qiazol Lysis Reagent, were processed for RNA purification and miRNA amplification (described below). Moreover, we evaluated the quality of isolated RNA cargo by assessing three EV preparations at different concentrations. Specifically, we tested two miRNAs (miR-132 and miR-203) and one lncRNA (MALAT1) by Real-Time RT-PCR in scalar dilutions of two single samples. Each dilution was analyzed with three technical replicate reactions. The schemes and description of the protocols are reported in the Supplementary Information.
Total RNA was extracted from FF-EV samples, still fixed on the NW platform, by using the miRNeasy mini kit (Qiagen, GmbH, Hilden, Germany), according to the manufacturer’s instructions, and finally eluted in a 30 μL total volume of RNase-free water. Due to the limited quantities of sample and according to previous papers, no quantification or quality control was carried out at this stage.17
4.5 μL of total RNA was used for miRNA-specific reverse transcription (RT) to obtain miRNA-specific cDNAs. 5μL of the cDNA total volume was analyzed with qRT-PCR using TaqMan MicroRNA Assays (Applied Biosystems). All qRT-PCR reactions were performed in 20 μL, containing 10 μL of TaqMan Universal PCR Master Mix (Applied Biosystems), 1 μL of miRNA-specific TaqMan MicroRNA Assay, 5 μL of RT product and 4 μL of nuclease-free water. We assayed three miRNAs (miR-212, miR-132 and miR-203), previously detected in FF.51 For each reaction, the following amplification profile was applied: 95 °C for 10 min for the first cycle; 95 °C for 15 sec and 60 °C for 1 m for 40 cycles.
For the analysis of longer RNA molecules, the lncRNA Metastasis Associated Lung Adenocarcinoma Transcript 1 (MALAT1) was selected by querying the public database ExoCarta (http://www.exocarta.org/) and literature data retrieval for genes expressed within the exosomes of FF. Primers for the MALAT1 lncRNA sequence were designed using Primer Blast software (Primer sequences are shown in Supplementary Material). Three different qRT-PCR reactions per sample were performed in a single step using 5 μL of total RNA and Power SYBR® Green RNA-to-Ct™ 1-StepKit (Applied Biosystems) according to the manufacturer’s protocol. The reaction was performed in the 7900 HT Fast Real-Time PCR System (Applied Biosystems) according to the following protocol: 48°C for 30 m for RT reaction, 95°C for 10 min for the first cycle, followed by 40 cycles of amplification (95°C for 15 seconds and 60°C for 1 m). Finally, 95°C for 15 sec and 60°C for 15 sec for melting curve analysis.
Simultaneously, complementary cDNA was synthesized from 5 μL of total RNA from three different FF-EV samples using SuperScript II Reverse Transcriptase (Invitrogen S.R.L.) and random hexamer primers (Roche Molecular Diagnostics) according to standard protocols. We performed 3 different PCR reactions by using Platinum Taq polymerase (Invitrogen).
For statistical analysis, Pearson’s Correlation coefficient (r) and P-value were calculated using GraphPad Prism 6. Expression data are shown as mean 2−Ct (threshold cycle) values ± SD (Standard deviation). The correlation between RNA inputs (1x, 2x and 4x) and each data point representing 2−Ct values were determined for miR-132, miR-203 and MALAT1.
Synthetic Vesicles Preparation
Synthetic vesicles were made of Soybean lecithin and were prepared according to the protocol reported in.53 Unilamellar vesicles with a diameter of 100 nm were obtained. Their concentration was estimated according to this work.
We acknowledge the project ADAS + ARS01_00459. The authors would like to thank the Scientific Bureau of the University of Catania for language support and Fondi di ateneo 2020–2022, University of Catania, Open Access line, and Project Piaceri-University of Catania.
The authors report no conflicts of interest in this work.
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