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A multichannel computer-driven system to raise aquatic embryos under selectable hypoxic conditions

Authors Metikala S, Neuhaus H, Hollemann T

Received 13 September 2017

Accepted for publication 2 November 2017

Published 12 January 2018 Volume 2018:6 Pages 1—9

DOI https://doi.org/10.2147/HP.S151536

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Akshita Wason

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Doerthe Katschinski


Sanjeeva Metikala,1,2,* Herbert Neuhaus,1,* Thomas Hollemann1

1Department of Medical Molecular Biology, University Halle-Wittenberg, Institute for Physiological Chemistry, Halle, Germany; 2Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, USA

*These authors contributed equally to this work

Abstract: The formation of a functional cardiovascular system is an essential step in the early vertebrate embryo. Nevertheless, the effect of hypoxia on the developmental program of organisms was studied rarely. In particular, this holds true for vertebrate embryos that depend on a functional placenta for proper development and had not been studied in this respect due to the obvious limitation. We established a protocol to culture aquatic embryos, which enabled us to culture a high number of Xenopus embryos until tadpole stage under defined hypoxic conditions in four hypoxia chambers simultaneously, employing a computerized system. In general, our results show that hypoxia results in delayed development and, in particular, we could show that oxygen availability was most crucial during gastrulation and organogenesis (early tailbud) phases during embryonic development of Xenopus laevis.

Keywords: Xenopus, hypoxia, vasculogenesis, culture conditions, er71, ami

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