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Transcriptome Analysis Reveals the Resistance Mechanism of Pseudomonas aeruginosa to Tachyplesin I

Authors Hong J, Jiang H, Hu J, Wang L, Liu R

Received 9 August 2019

Accepted for publication 17 December 2019

Published 16 January 2020 Volume 2020:13 Pages 155—169

DOI https://doi.org/10.2147/IDR.S226687

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 5

Editor who approved publication: Dr Sahil Khanna


Video abstract presented by Jun Hong.

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Jun Hong, 1, 2 Honghao Jiang, 1 Jianye Hu, 1 Lianzhe Wang, 1 Ruifang Liu 1

1College of Life Science and Engineering, Henan University of Urban Construction, Ping Dingshan 467036, People’s Republic of China; 2Henan Province Key Laboratory of Water Pollution Control and Rehabilitation Technology, Henan University of Urban Construction, Pingdingshan, Henan 467036, People’s Republic of China

Correspondence: Lianzhe Wang
College of Life Science and Engineering, Henan University of Urban Construction, Ping Dingshan 467036, People’s Republic of China
Tel +86-180 3758 0131
Email jijy99@126.com

Background: Tachyplesin I is a cationic antimicrobial peptide with a typical cyclic antiparallel β-sheet structure. We previously demonstrated that long-term continuous exposure to increased concentration of tachyplesin I can induce resistant Gram-negative bacteria. However, no significant information is available about the resistance mechanism of Pseudomonas aeruginosa (P. aeruginosa) to tachyplesin I.
Materials and Methods: In this study, the global gene expression profiling of P. aeruginosa strain PA-99 and P. aeruginosa CGMCC1.2620 (PA1.2620) was conducted using transcriptome sequencing. For this purpose, outer membrane permeability and outer membrane proteins (OMPs) were further analyzed.
Results: Transcriptome sequencing detected 672 upregulated and 787 downregulated genes, covering Clusters of Orthologous Groups (COGs) of P. aeruginosa strain PA-99 compared with PA1.2620. Totally, 749 differentially expressed genes (DEGs) were assigned to 98 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and among them, a two-component regulatory system, a beta-lactam resistance system, etc. were involved in some known genes resistant to drugs. Additionally, we further attempted to indicate whether the resistance mechanism of P. aeruginosa to tachyplesin I was associated with the changes of outer membrane permeability and OMPs.
Conclusion: Our results indicated that P. aeruginosa resistant to tachyplesin I was mainly related to reduced entry of tachyplesin I into the bacterial cell due to overexpression of efflux pump, in addition to a decrease of outer membrane permeability. Our findings were also validated by pathway enrichment analysis and quantitative reverse transcription polymerase chain reaction (RT-qPCR). This study may provide a promising guidance for understanding the resistance mechanism of P. aeruginosa to tachyplesin I.

Keywords: antibiotic resistance, tachyplesin I, P. aeruginosa, differentially expressed genes, outer membrane permeability, outer membrane proteins

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