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Three-dimensional tumor cell cultures employed in virotherapy research

Authors Kloker LD, Yurttas C, Lauer UM

Received 13 February 2018

Accepted for publication 12 May 2018

Published 5 September 2018 Volume 2018:7 Pages 79—93

DOI https://doi.org/10.2147/OV.S165479

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Amy Norman

Peer reviewer comments 4

Editor who approved publication: Dr Chae-Ok Yun


Linus D Kloker,1 Can Yurttas,2 Ulrich M Lauer1,3

1Department of Clinical Tumor Biology, University Hospital, University of Tübingen, Tübingen, Germany; 2Department of General, Visceral and Transplant Surgery, University Hospital, University of Tübingen, Tübingen, Germany; 3German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Tübingen, Germany

Abstract: Oncolytic virotherapy constitutes an upcoming alternative treatment option for a broad spectrum of cancer entities. However, despite great research efforts, there is still only a single US Food and Drug Administration/European Medicines Agency-approved oncolytic virus available for clinical use. One reason for that is the gap between promising preclinical data and limited clinical success. Since oncolytic viruses are biological agents, they might require more realistic in vitro tumor models than common monolayer tumor cell cultures to provide meaningful predictive preclinical evaluation results. For more realistic invitro tumor models, three-dimensional tumor cell-culture systems can be employed in preclinical virotherapy research. This review provides an overview of spheroid and hydrogel tumor cell cultures, organotypic tumor-tissue slices, organotypic raft cultures, and tumor organoids utilized in the context of oncolytic virotherapy. Furthermore, we also discuss advantages, disadvantages, techniques, and difficulties of these three-dimensional tumor cell-culture systems when applied specifically in virotherapy research.

Keywords: oncolytic virotherapy, oncolytic viruses, three-dimensional cell culture, tumor modeling, preclinical testing

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