Back to Journals » International Journal of Nanomedicine » Volume 6

Thiolated chitosan nanoparticles for enhancing oral absorption of docetaxel: preparation, in vitro and ex vivo evaluation

Authors Shahrooz Saremi, Fatemeh Atyabi, Seyedeh Parinaz Akhlaghi, et al

Published 12 January 2011 Volume 2011:6 Pages 119—128

DOI https://doi.org/10.2147/IJN.S15500

Review by Single-blind

Peer reviewer comments 2

Shahrooz Saremi1,4, Fatemeh Atyabi1,2, Seyedeh Parinaz Akhlaghi1, Seyed Nasser Ostad3, Rassoul Dinarvand1,2
1Department of Pharmaceutics, 2Nanotechnology Research Centre, 3Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; 4R&D Department, Osvah Pharmaceutical Co., Tehran, Iran

Abstract: The aim of this study was to prepare and evaluate mucoadhesive core-shell nanoparticles based on copolymerization of thiolated chitosan coated on poly methyl methacrylate cores as a carrier for oral delivery of docetaxel. Docetaxel-loaded nanoparticles with various concentrations were prepared via a radical emulsion polymerization method using cerium ammonium nitrate as an initiator. The physicochemical properties of the obtained nanoparticles were characterized by: dynamic light-scattering analysis for their mean size, size distribution, and zeta potential; scanning electron microscopy and transmission electron microscopy for surface morphology; and differential scanning calorimetry analysis for confirmation of molecular dispersity of docetaxel in the nanoparticles. Nanoparticles were spherical with mean diameter below 200 nm, polydispersity of below 0.15, and positive zeta potential values. The entrapment efficiency of the nanoparticles was approximately 90%. In vitro release studies showed a sustained release characteristic for 10 days after a burst release at the beginning. Ex vivo studies showed a significant increase in the transportation of docetaxel from intestinal membrane of rat when formulated as nanoparticles. Cellular uptake of nanoparticles was investigated using fluoresceinamine-loaded nanoparticles. Docetaxel nanoparticles showed a high cytotoxicity effect in the Caco-2 and MCF-7 cell lines after 72 hours. It can be concluded that by combining the advantages of both thiolated polymers and colloidal particles, these nanoparticles can be proposed as a drug carrier system for mucosal delivery of hydrophobic drugs.

Keywords: poly methyl methacrylate, mucoadhesive, cytotoxicity

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Other articles by this author:

Chitosan–Pluronic nanoparticles as oral delivery of anticancer gemcitabine: preparation and in vitro study

Hosseinzadeh H, Atyabi F, Dinarvand R, Ostad SN

International Journal of Nanomedicine 2012, 7:1851-1863

Published Date: 11 April 2012

Poly (ε-caprolactone) nanofibrous ring surrounding a polyvinyl alcohol hydrogel for the development of a biocompatible two-part artificial cornea

Bakhshandeh H, Soleimani M, Shah Hosseini S, Hashemi H, Shabani I, Shafiee A, Behesht Nejad AH, Erfan M, Dinarvand R, Atyabi F

International Journal of Nanomedicine 2011, 6:1509-1515

Published Date: 14 July 2011

Polyanionic carbohydrate doxorubicin–dextran nanocomplex as a delivery system for anticancer drugs: in vitro analysis and evaluations

Yousefpour P, Atyabi F, Vasheghani Farahani E, Sakhtianchi R, Dinarvand R

International Journal of Nanomedicine 2011, 6:1487-1496

Published Date: 11 July 2011

Increased paclitaxel cytotoxicity against cancer cell lines using a novel functionalized carbon nanotube

Sobhani Z, Dinarvand R, Atyabi F, Ghahremani M, Adeli M

International Journal of Nanomedicine 2011, 6:705-719

Published Date: 5 April 2011

Readers of this article also read:

Lipid-coated gold nanocomposites for enhanced cancer therapy

Kang JH, Ko YT

International Journal of Nanomedicine 2015, 10:33-45

Published Date: 25 August 2015

Green synthesis of water-soluble nontoxic polymeric nanocomposites containing silver nanoparticles

Prozorova GF, Pozdnyakov AS, Kuznetsova NP, Korzhova SA, Emel’yanov AI, Ermakova TG, Fadeeva TV, Sosedova LM

International Journal of Nanomedicine 2014, 9:1883-1889

Published Date: 16 April 2014

Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon

Ashwanikumar N, Kumar NA, Nair SA, Kumar GS

International Journal of Nanomedicine 2012, 7:5769-5779

Published Date: 15 November 2012

Cross-linked acrylic hydrogel for the controlled delivery of hydrophobic drugs in cancer therapy

Deepa G, Thulasidasan AK, Anto RJ, Pillai JJ, Kumar GS

International Journal of Nanomedicine 2012, 7:4077-4088

Published Date: 27 July 2012

Improved performance of diatomite-based dental nanocomposite ceramics using layer-by-layer assembly

Lu X, Xia Y, Liu M, Qian Y, Zhou X, Gu N, Zhang F

International Journal of Nanomedicine 2012, 7:2153-2164

Published Date: 24 April 2012

Degradable gene delivery systems based on Pluronics-modified low-molecular-weight polyethylenimine: preparation, characterization, intracellular trafficking, and cellular distribution

Fan W, Wu X, Ding B, Gao J, Cai Z, Zhang W, Yin D, Wang X, Zhu Q, Liu J, Ding X, Gao S

International Journal of Nanomedicine 2012, 7:1127-1138

Published Date: 24 February 2012

Crystallization after intravitreal ganciclovir injection

Pitipol Choopong, Nattaporn Tesavibul, Nattawut Rodanant

Clinical Ophthalmology 2010, 4:709-711

Published Date: 14 July 2010