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The Relationship Between Single Nucleotide Polymorphisms of SMAD3/SMAD6 and Risk of Esophageal Squamous Cell Carcinoma in Chinese Population

Authors Yu J, Dong Y, Tang W, Pan H, Lv L, Long T, Zhou Q, Qi J, Liu J, Ding G, Yin J, Tan L

Received 16 February 2020

Accepted for publication 20 July 2020

Published 24 August 2020 Volume 2020:13 Pages 355—363


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Martin Bluth

Jinjie Yu,1,* Yunpeng Dong,1,* Weifeng Tang,2 Huiwen Pan,2 Lu Lv,2 Tao Long,2 Qiang Zhou,2 Junqing Qi,2 Jianchao Liu,2 Guowen Ding,2 Jun Yin,1 Lijie Tan1

1Department of Thoracic Surgery, Zhongshan Hospital of Fudan University, Shanghai, People’s Republic of China; 2Department of Cardiothoracic Surgery, Affiliated People’s Hospital of Jiangsu University, Jiangsu, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Jun Yin; Lijie Tan
Department of Thoracic Surgery, Zhongshan Hospital of Fudan University, No. 180, Fenglin Road, Xuhui District, Shanghai, People’s Republic of China
Tel +86 13917483128
; +86 13681972151

Background: The TGF-β signal pathways play a key role in the development and promotion of squamous cell carcinoma (SCC). The pathway is mediated by the SMAD family proteins that include SMAD3 and SMAD6. Our study aimed to evaluate the relationship between single nucleotide polymorphism (SNP) of SMAD3/SMAD6 and susceptibility to esophageal squamous cell carcinoma (ESCC) in the Chinese population.
Patients and Methods: This was a hospital-based case–control study compromised of 1043 ESCC patients and 1315 non-cancer patients. Seven SMAD3/SMAD6 (rs8028147, rs3743343, rs3743342, rs8025774, rs8031440, rs803167, and rs34643453) SNPs were selected and used to evaluate their correlation with ESCC susceptibility. Genetic model tests, stratified analyses, linkage disequilibrium analyses, and haplotype analyses were performed in our study.
Results: Participants with SMAD3 rs3743342 C>T, rs8025774 C>T, rs8031440 G>A or rs8031627 G>A had a significantly higher risk of ESCC. This was more evident in males, older patients (> 63 years), smokers, and non-alcohol drinking participants. Linkage disequilibrium analyses further revealed that there were strong correlations between SMAD3 rs3743342 C>T, rs8025774 C>T, rs8031440 G>A, and rs8031627 G>A. In the same line, haplotype analyses revealed that SMAD3 ACCCGGSMAD6A and SMAD3AGCCGGSMAD6A were associated with less susceptibility to ESCC while SMAD3ATTTAASMAD6A was associated with a higher risk of ESCC.
Conclusion: SNPs of SMAD3 were related to higher susceptibility to ESCC. As such, they may contribute to the development of viable strategies for early diagnosis and treatment of ESCC. However, more detailed association mechanisms between SMAD3/SMAD6 SNPs and ESCC need further experiments to prove.

Keywords: SMAD, single nucleotide polymorphism, esophageal squamous cell carcinoma

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