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TGF-β1 Gene Polymorphism and Its Correlation with Serum Level of TGF-β1 in Psoriasis Vulgaris Among Iraqi People

Authors Ahmed BT, Saeed MY, Noori SH, Amin DM

Received 16 September 2020

Accepted for publication 18 November 2020

Published 24 November 2020 Volume 2020:13 Pages 889—896

DOI https://doi.org/10.2147/CCID.S281585

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Jeffrey Weinberg


Bryar T Ahmed,1 Mohammad Y Saeed,1 Saman H Noori,2 Dashty M Amin3

1Department of Medicine/Dermatology, College of Medicine, University of Sulaimani, Sulaimani City, Kurdistan, Iraq; 2Department of Biochemistry, College of Medicine, University of Sulaimani, Sulaimani City, Kurdistan, Iraq; 3Medical Laboratory Sciences, Komar University of Science and Technology, Sulaimani City, Kurdistan, Iraq

Correspondence: Bryar T Ahmed
Department of Medicine/Dermatology, College of Medicine, University of Sulaimani, P.O Box 97, Hawarabarza, Sulaimani City, Iraq
Tel +9647701520468
Email bryarbarawi@hotmail.com

Purpose: Many cytokines have been implicated in the pathogenesis of psoriasis, among these the transforming growth factor-beta 1 (TGF-β 1) can be endorsed by different mechanisms besides inhibiting keratinocytes proliferation. The role of genetic polymorphisms of TGF-β 1 has been studied in various inflammatory diseases. Our aim is to study the correlation of TGF-β 1 gene polymorphism at codon 10 and 25 with the expression of serum level of TGF-β 1 in a sample of Iraqi psoriatic patients compared to the control group.
Materials and Methods: A cross-sectional study involved 100 patients with psoriasis vulgaris and 50 sex- and age-matched healthy volunteers as control group. Serum and genomic DNA were prepared from peripheral blood samples. Amplification refractory mutation system–polymerase chain reaction technique (ARMS-PCR) had been applied for genotyping TGF-β 1 codon 10 [rs1982073] and codon 25 [rs1800471] genetic polymorphisms. Enzyme-linked immunosorbent assay technique (ELISA) based on the sandwich principle was used for quantification of serum TGF-β 1 level. Psoriasis Area and Severity Index (PASI) scoring was applied for determining the severity in psoriatic patients and classified accordingly to mild (PASI< 7), moderate (PASI 7– 12), severe (PASI> 12) groups.
Results: Statistically significant difference was found in TGF-β 1 gene polymorphism between psoriatic patients and control group at codon 10 (T869C) polymorphism (p=0.021) and codon 25 (G915C) polymorphism (p=0.040). No significant association was detected with the mean serum TGF-β 1 level, severity of the disease, disease onset, gender, history of psoriatic arthritis, and smoking in both codons. Significant lower mean serum TGF-β 1 level was found among psoriatic group (192.17 ± 531.12 ng/L) compared with controls (565.89 ± 1372.30 ng/L) (p = 0.018). Relation of mean serum TGF-β 1 level with the onset of the disease was statistically significant (p = 0.004), early-onset disease group was lower (105.92 ± 68.02 ng/L) compared with the late-onset disease group (450.92 ± 1027.79 ng/L). The mean serum TGF-β 1 level showed no significant differences with the severity of psoriasis, gender, history of psoriatic arthritis, and smoking.
Conclusion: Iraqi population showed a significant association between TGF-β 1 gene polymorphism at codon 10 and 25 were with psoriasis susceptibility, and a significantly lower mean serum TGF-β 1 level was detected in psoriatic patients.

Keywords: psoriasis, TGF-β 1, gene, polymorphism

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