Back to Journals » International Journal of Nanomedicine » Volume 10 » Issue 1

Temperature-dependent and time-dependent effects of hyperthermia mediated by dextran-coated La0.7Sr0.3MnO3: in vitro studies

Authors Haghniaz R, Umrani R, Paknikar K

Received 26 November 2014

Accepted for publication 18 January 2015

Published 25 February 2015 Volume 2015:10(1) Pages 1609—1623


Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 4

Editor who approved publication: Prof. Dr. Thomas J Webster

Reihaneh Haghniaz, Rinku D Umrani, Kishore M Paknikar

Centre for Nanobioscience, Agharkar Research Institute, Pune, Maharashtra, India

Background: The purpose of this study was to investigate the therapeutic efficacy of dextran-coated (Dex) La0.7Sr0.3MnO3 (LSMO) nanoparticles-mediated hyperthermia at different temperatures (43°C, 45°C, and 47°C) based on cell killing potential and induction of heat shock proteins in a murine melanoma cell (B16F1) line.
Methods: LSMO nanoparticles were synthesized by a citrate-gel method and coated with dextran. B16F1 cells were exposed to the Dex-LSMO nanoparticles and heated using a radiofrequency generator. After heating, the morphology and topology of the cells were investigated by optical microscopy and atomic force microscopy. At 0 hours and 24 hours post heating, cells were harvested and viability was analyzed by the Trypan blue dye exclusion method. Apoptosis and DNA fragmentation were assessed by terminal deoxynucleotidyl transferase-dUTP nick end labeling (TUNEL) assay and agarose gel electrophoresis, respectively. An enzyme-linked immunosorbent assay was used to quantify heat shock protein levels.
Results: Our data indicate that cell death and induction of heat shock proteins in melanoma cells increased in a time-dependent and temperature-dependent manner, particularly at temperatures higher than 43°C. The mode of cell death was found to be apoptotic, as evident by DNA fragmentation and TUNEL signal. A minimum temperature of 45°C was required to irreversibly alter cell morphology, significantly reduce cell viability, and result in 98% apoptosis. Repeated cycles of hyperthermia could induce higher levels of heat shock proteins (more favorable for antitumor activity) when compared with a single cycle.
Conclusion: Our findings indicate a potential use for Dex-LSMO-mediated hyperthermia in the treatment of melanoma and other types of cancer.

Keywords: hyperthermia, Dex-LSMO nanoparticles, heat shock proteins, melanoma, apoptosis

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]


Other article by this author:

Hyperthermia mediated by dextran-coated La0.7Sr0.3MnO3 nanoparticles: in vivo studies

Haghniaz R, Umrani RD, Paknikar KM

International Journal of Nanomedicine 2016, 11:1779-1791

Published Date: 27 April 2016

Readers of this article also read:

Role of surface charge in determining the biological effects of CdSe/ZnS quantum dots

Liu QQ, Li HX, Xia QY, Liu Y, Xiao K

International Journal of Nanomedicine 2015, 10:7073-7088

Published Date: 16 November 2015

Formation of curcumin nanoparticles via solution-enhanced dispersion by supercritical CO2

Zhao Z, Xie M, Li Y, Chen A, Li G, Zhang J, Hu H, Wang X, Li S

International Journal of Nanomedicine 2015, 10:3171-3181

Published Date: 29 April 2015

Single-step green synthesis and characterization of gold-conjugated polyphenol nanoparticles with antioxidant and biological activities

Sanna V, Pala N, Dessì G, Manconi P, Mariani A, Dedola S, Rassu M, Crosio C, Iaccarino C, Sechi M

International Journal of Nanomedicine 2014, 9:4935-4951

Published Date: 23 October 2014

Use of electrical impedance spectroscopy to detect malignant and potentially malignant oral lesions

Murdoch C, Brown BH, Hearnden V, Speight PM, D'Apice K, Hegarty AM, Tidy JA, Healey TJ, Highfield PE, Thornhill MH

International Journal of Nanomedicine 2014, 9:4521-4532

Published Date: 23 September 2014

Entrapment of curcumin into monoolein-based liquid crystalline nanoparticle dispersion for enhancement of stability and anticancer activity

Baskaran R, Madheswaran T, Sundaramoorthy P, Kim HM, Yoo BK

International Journal of Nanomedicine 2014, 9:3119-3130

Published Date: 26 June 2014

In situ precipitation: a novel approach for preparation of iron-oxide magnetoliposomes

Xia S, Li P, Chen Q, Armah M, Ying X, Wu J, Lai J

International Journal of Nanomedicine 2014, 9:2607-2617

Published Date: 23 May 2014