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Synthesis and preliminary characterization of polyurethane nanoparticles with ginger extract as a possible cardiovascular protector

Authors Borcan F, Chirita-Emandi A, Andreescu NI, Borcan LC, Albulescu RC, Puiu M, Tomescu MC

Received 18 January 2019

Accepted for publication 6 March 2019

Published 21 May 2019 Volume 2019:14 Pages 3691—3703

DOI https://doi.org/10.2147/IJN.S202049

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Thomas J. Webster

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Florin Borcan,1 Adela Chirita-Emandi,2,3 Nicoleta Ioana Andreescu,2,3 Livia-Cristina Borcan,4 Ramona Carmen Albulescu,5 Maria Puiu,2,3 Mirela Cleopatra Tomescu4

1The 1st Department (Analytical Chemistry), Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania; 2The 2nd Department (Genetics), Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania; 3Center of Genomic Medicine, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania; 4The 5th Department (Internal Medicine I), Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania; 5The 11th Department (Pediatrics), Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania

Background and aim: The extract of ginger, obtained from the rhizome of Zingiber officinale, contains 6-gingerol, 6-shogaol, 8-gingerol, and 10-gingerol. It has many therapeutic effects such as being chemopreventive against stroke and heart diseases, malabsorption, bacterial infections, indigestion, and nausea, which have been observed since ancient times. The main aim of this study is to evaluate the polyurethane (PU) as a proper material for the hollow nanoparticles’ preparation.
Methods: The PU nanoparticles were obtained by a spontaneous emulsification, in the presence of a nonionic surfactant, combined with an interfacial polyaddition process between an aliphatic diisocyanate and different mixtures of etheric and esteric polyols. The synthesis was done without any PU additives, such as catalysts, blowing agents, chains promoters, cross-linking agents, and stabilizers.
Results: The particles present almost neutral pH values and low water solubility. They are heat resistant up to 280°C. Decreased irritation level was found in the assay of PU nanoparticles loaded with pure ginger extract (GE) on the murine skin tests than the irritation level recorded for pure GE.
Conclusion: This research shows the reduced noxiousness of these PU nanoparticles and consequently the possibility of their use as a possible cardiovascular protector.

Keywords: CD1 Nu/Nu mice, drug carrier, hollow nanoparticles, noxiousness, Zeta potential

Introduction

Cardiovascular diseases are disorders of heart and blood vessels including coronary artery disease, cerebrovascular disease, or peripheral arterial disease.1 Cardiovascular diseases are the leading cause of mortality in the world. Elevated levels of cholesterol and triglycerides, increased percentage of fat, and decreased percentage of muscle mass which are not counterbalanced by an efficient metabolism and a regular physical activity are favorable factors for cardiovascular diseases.2

Phytotherapy is a therapeutic method which uses medicinal plants to ameliorate disease. This kind of therapy is a thousand years old and has already proven its efficiency. Unlike allopathic medicine, which recommends only substances extracted from plants, phytotherapy uses plants as total extracts. Various parts of the plant are used – together or separately, as appropriate – from roots to flowers, including bark and buds.3 The following main categories of phytocompounds are used in different therapies: flavonoids (rutin, quercetin, epicatechin, kaempferol, etc.), phenolic acids (capsaicin, rosmarinic acid, and tannic acid), hydroxycinnamic acid (coumarin and ferulic acid), organosulfides (allicin, glutathione, and sulforaphane), alkaloids (theophylline and caffeine), carotenoids (beta-carotene and lycopene), anthocyanins, terpenes, isoflavones, and phytosterols.4 Vasanthi et al described that a part of isoflavones, diosgenin, resveratrol, quercetin, catechin, sulforaphane, tocotrienols, and carotenoids were already found to reduce the risk of cardiovascular diseases and aid in cardioprotection by their antioxidative, antihypercholesteroemic, antiangiogenic, anti-ischemic, inhibition of platelet aggregation, and anti-inflammatory activities.5

Polyurethane (PU) materials appear for the first time 80 years ago in I.G. Farbenindustrie Laboratories from Leverkusen; Prof. O. Bayer obtained PU foams from toluene diisocyanate and polyester polyols.6 The use of PU in the field of drug delivery is relatively recent: Hong and Park developed the first PU microcarrier two decades ago.7 We already obtained PU nano- and microstructures with different shapes and sizes in the last 5 years.812 The aim of this study is to obtain and to characterize a mixture of polyester- and polyether-urethanes which can assure a constant release of apure ginger extract (GE) for a prolonged time.

Material and methods

Reagents

The characteristics of the main commercial reagents are presented in Table 1. Reagents were kept under the conditions indicated by the manufacturer, and they were used without further purifications.

Table 1 The main characteristics of the reagents

Obtaining of GE

Munteanu et al described the procedure which was chosen for obtaining the GE;10 the ginger root was crushed using a chisel, and it was rapidly dried at 90°C for 2 days and extracted with 70% ethanol for 24 hrs. The obtained mixture was then filtered, centrifuged at 25°C with 2,000 rpm for 5 mins, and concentrated using a rotavapor at 90°C until constant weight.

Synthesis of nanoparticles

The procedure chosen for the synthesis is based on an interfacial polyaddition reaction (Figure 1) combined with a spontaneous emulsification; the protocol presents the following steps:11,12

  1. Preparation of the aqueous phase (PU soft segment) – a mixture of BD, PEG and PCL, Tween® 20, and distilled water 0.5:1:1:30 was heated at 35°C and homogenized in an Erlenmeyer flask (100 mL);
  2. Preparation of the organic phase (PU hard segment) – a mixture of LDI and acetone 1:15 was heated at 35°C and homogenized in a Berzelius beaker (50 mL);
  3. The organic component was injected into the aqueous one under magnetic stirring (400 rpm) and heated (35°C). The PU nanoparticles start to appear in this moment as a white precipitate. However, the stirring is maintained for 3 hrs at 40°C in order to ensure the completion of all chemical reactions (maturation time);
  4. The obtained suspensions were repeatedly washed with a mixture (acetone–water 3:1 v/v) in order to eliminate unwanted/secondary products such as amines;
  5. The solvent and a part of water were removed by slow evaporation, keeping the suspension as thin layers at 50°C for around 72 hrs.

Figure 1 The chemical reaction used for the synthesis of polyurethane particles.

The procedure previ ously described was repeated six times using different PEG/PCL ratios and with/without GE. Table 2 presents the main raw materials used for the two sample: sample PU_0 is a mixture 1:1:1 (w/w) of the first three syntheses’ products, while PU_1 combines the last three syntheses’ products at the same ratio, because the combining of samples with different polyether/polyester ratios leads to products containing particles with different degradation speed and assures an approximately constant concentration of drug released.

Table 2 The raw materials for PU nanoparticle synthesis

pH and solubility measurements

The preliminary characterization of PU nanoparticles with and without GE starts by a series of dissolution tests. Distilled water, ethanol, acetone, and DMSO were used as solvents, following a procedure described in ASTM D 3132-84.13

The pH of PU nanoparticles samples was evaluated using a portable pH Meter Checker® (Hanna Instruments, Woonsocket, RI, USA) and aqueous solutions with the same concentration (0.8 mg/mL) at the same temperature (25°C). The instrument was previously calibrated using four different buffer solutions (pH=3, 5, 7, and 10).

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis

The MALDI-TOF technique was used to determine any change in the nanoparticle chemistry following the addition of GE during the synthesis. Gingerol, as the main constituent of GE, is a chemical compound with two hydroxyl groups, and it can be a chain extender during the particle synthesis.

The Bruker HCCA (alpha-cyano-4-hydroxycinnamic acid) matrix (5 mg/mL in a mixture of acetonitrile, water, and trifluoroacetic acid) was used to suspend the nanoparticles. 1 µL suspension was added onto the target plate (MTP 384 ground steel, Bruker Daltonics, Bremen, Germany). The spots were allowed to dry at room temperature.

The mass spectra were obtained using UltrafleXtreme MALDI TOF/TOF instrument (Bruker Daltonics). The analysis was performed in positive ion reflectron mode in a mass range of 700–3,500 m/z. The instrument was calibrated using Bruker Peptide Calibration Standard I containing mixture of seven standard peptides. Flex Control® (version 3.4) software was used to acquire the data and set the method parameters. The following settings were used: laser frequency – 1,000 Hz; smartbeam - “4_large”; sample rate and digitiser settings – 2.50 GS/s; accelerator voltage – 20.12 kW; extraction voltage – 17.89 kW; lens voltage – 7.23 kW; delayed extraction – 120 ns; and reflector voltage – 20.23 kW. The laser intensity was adjusted such that the highest peak in the spectrum was in the range of 104 arbitrary units. 1,000 laser shots were used for each individual spectrum and minimum 10 individual spectra (10,000 laser shots) were cumulated and saved.

The mass spectra were processed using Flex Analysis® software (version 3.4, Bruker Daltonics).

Yield of encapsulation

Bouchemal et al described a procedure to evaluate the amount of an active agent entrapped inside PU nanocapsules; the protocol is based on the determination of free/untrapped drug using its UV-Vis absorption related to the quantity of this drug added into synthesis.14

The formula is: Yield of encapsulation = (1–Quantity of free drug/Quantity of drug added)⨰100.

Thermal analysis

The thermal behavior of samples containing PU nanoparticles was studied by differential scanning calorimetry (DSC). Small amounts of samples, between 3.4 and 3.7 mg, were placed inside aluminum crucibles with pierced caps, and they were heated between 30°C and 280°C in an inert atmosphere (a flow rate of 100 mL/min Ar was used to prevent sample contamination and oxidation during the DSC runs) with a heating speed equal with 5 degree/min. A reference material (empty aluminum crucible with pierced cap) simultaneously undergoes the same routine. The instrument, a DSC1 (Mettler-Toledo, Greifensee, Switzerland), was previously calibrated with the use of Zn and In as standards.

Zetasizer measurements

The size and the surface charge of the PU nanoparticles with and without GE were evaluated using a Cordouan Zetasizer (Cordouan Technologies, Pessac, France). This instrument contains two different analyzers: a Vasco Particle Size Analyzer and a Wallis Zetapotential Analyzer. The following parameters were set in the case of Vasco Analyzer: working temperature (25°C); time intervals (22 μs); number of channels (410±20); laser power (80±5%); acquisition mode (continuous) and analysis mode (Pade-Laplace). In the case of Wallis Zetapotential Analyzer (Cordouan Technologies, Pessac, France), the parameters were: plastic cuvette with applicability between 380 and 780 nm; temperature (25°C); laser power (75±5%); applied field (automatic); resolution (0.8 Hz - medium); 3 measures/sequence, and Henry function (Smoluchowski).

Test of membrane penetration

The penetrability of polymer nanoparticles with and without GE through membranes was tested using a PVDF (polyvinylidene fluoride) artificial membrane Spectra/Por®:15 2.0 mL sample was loaded inside a dialysis tube and 100 mL saline buffer were used in the external chamber. The experiment was done at room temperature and at every 10 hrs, 1.0 mL liquid from the external chamber was replaced with fresh buffer, and it was analyzed by comparing the absorbance recorded on a UVi Line 9400 (SI Analytics, Mainz, Germany) at 290 nm.

In vivo evaluations

Twelve CD1 Nu/Nu, 2-month-old, female mice were purchased from Charles River (Budapest, Hungary) and they were divided into four equal groups: group B (mice used as blank, treated with solvent), group PU_0 (mice treated with PU nanoparticles solution), group PU_1 (mice treated with solution of particles loaded with GE), and group G (mice treated with pure GE).

The solutions were applied on the back skin every third day (0.5 mL/application) and the determinations of skin parameters were performed within 20 mins after each application. All measurements were carried out according to the published guidelines16 with a Multiprobe Adapter System (MPA5) from Courage&Khazaka Electronics, Germany, equipped with a Tewameter® TM300 probe and a Mexameter® MX18 probe, at the same moment of the day, by the same operator, in a narrow range of temperature (22±1 °C) and air humidity (40±3%).

Human and animal skin has a crucial role in the protection of the body against different pathogens.17 The Tewameter® TM300 probe is designed to evaluate the barrier role of skin. The transepidermal water loss (TWL) is a natural process whose important increases show a disruption of the skin barrier (wounds, scratches, burns, exposure to solvents or surfactants, and extreme dryness) and it was reported as a good parameter to predict the irritation potential of a given compound.18

On the other hand, mexametry is a technique used to determine the two components of skin color: melanin and hemoglobin (erythema).19 The Mexameter® MX18 probe emits green, red, and infrared and the quantity of light absorbed by the skin is calculated in just 1–3 s.

Statistics and ethical approval

All statistical analyses were performed using a trial version of IBM SPSS. The measurements from this study were done in triplicate, and the results are expressed as mean ± SE. One-way ANOVA and Bonferonni–Dunn tests were used to determine the statistical difference between experimental and blank groups; *, ** and *** indicate p<0.05, p<0.01, and p<0.001.

All investigations were done according to the declaration of Helsinki principles. Authors declare that all procedures involving animal subjects respect the specific regulations and standards: this study was first evaluated and approved by the Ethical Committee of “Victor Babes” University of Medicine and Pharmacy Timisoara, Romania. The work protocol followed the rules of the National Institute of Animal Health: during the experiment, animals were maintained in standard conditions of 12 hr light–dark cycle, food, and water ad libitum, at 25±1°C and humidity >55%.

Results

Evaluation of samples stability

The stability of samples was first monitored after their syntheses in a similar manner to that described by Borcan et al:11 a part of every sample was separated after purification, and it was divided and maintained at 3 different temperatures (8±0.1°C in a refrigerator, 25±0.1 and 40±0.1 in a laboratory incubator) for 30 days. The aspect including the color of samples was stable all this period.

pH and solubility measurements

Table 3 presents the pH and solubility values of samples containing PU particles without and with GE (PU_0 and PU_1). Increased solubility values of PU sample with GE were observed in water, ethanol, and acetone, and this may be due to the fact that PU chains may have a different number of free hydroxyl groups depending on the entrapped drug.

Table 3 pH and solubility of samples in different solvents

MALDI-TOF MS analysis

The main component of our synthesis (PEG) can be observed on MALDI-TOF MS spectra (Figure 2). Both samples (PU_0 and PU_1) present similar spectra with an average m/z of 1,420. In both samples, the most intense peak was observed at 1,199 m/z. The adjacent peaks differ in mass by one monomer unit (average of 44.5 m/z). No significant differences were observed between the two samples, and therefore no change was detected in the particle chemistry, when loaded with the extract.

Figure 2 MALDI-TOF spectra of polyurethane particles.

Ketata et al have described in their paper that PU fragmentation is similar to its thermal decomposition; the recast of the raw materials (polyol and diisocyanate) is the main pathway of these degradation processes.20

Yield of encapsulation

The maximum absorbance of PU samples is between 300 and 310 nm, while GE presents a peak at 386 nm (Figure 3). This wavelength was chosen to evaluate the amount of free active substance; four solutions with different concentrations were prepared, and a graph absorbance vs calibration was drawn (R2=0.997). An encapsulation efficiency equal with 82.9% was obtained by reporting the amount of free drug to the quantity of all GE added to synthesis.

Figure 3 UV-Vis spectra of polyurethane particles vs ginger extract.

Thermal analysis

DSC curves indicate the melting and boiling point of an unknown sample (endothermic phase transitions), a crystallization (an exothermic process), and other material parameters such as the vitreous transition of polymers, etc.,21 The PU nanoparticles are stable within the studied temperature range. Bolcu et al have described that PU decomposition starts at around 300±20°C depending on the aliphatic/aromatic character of diisocyanate radicals.22 No important difference was observed between the sample with and without GE (Figure 4).

Figure 4 DSC thermograms of polyurethane samples. Abbreviations: DSC, differential scanning calorimetry; PU, polyurethane; PU_0, polyurethane sample without ginger extract; PU_1, polyurethane sample with ginger extract; Lab, laboratory; UMFT, University of Medicine and Pharmacy Timisoara; exo, exoterm.

Zetasizer measurements

Table 4 presents the size and surface charge values of the PU nanoparticles without and with GE (PU_0 and PU_1).

Table 4 Zetasizer characterization of synthesized samples

Munteanu et al have presented that colloidal suspensions with zeta potential values >30 mV are exceptionally stable, while particles with zeta potential between 20 and 30 mV have a medium tendency to form clusters in order to increase their stability.10 The particle sizes are <100 nm; thus, it can be appreciated that this carrier contains nanoparticles.

Test of membrane penetration

The nanoparticles play an important role in modern medicine due to their increased mobility, chemical reactivity, and energy absorption. Murthy wrote about quantum dots and their applications in optical images, superparamagnetic iron oxide nanoparticles in magnetic resonance imaging, and polymer- and liposome-based nanoparticles used in drug and gene delivery.23

Their increased mobility allows passing different membranes. However, the transmembrane transport is limited to their size, shape, and surface properties. The membrane penetration was evaluated using the dialysis procedure, and the variation of GE concentration was calculated by Beer–Lambert law at 386 nm (Figure 5).

Figure 5 The evolution of ginger extract amount passed through a membrane.

The PU nanoparticle degradation starts with a delay of 2 days, and a maximum concentration of GE passed through an artificial membrane was obtained after 3–4 days. This situation is often met in the case of PEGylated particles used as drug carriers; polyether-based particles present a delayed degradation due to their increased stability against hydrolysis.

In vivo evaluations

The skin is a sensitive organ that modifies its parameters during different experiments. The skin tissue is different from one person to another and it depends on the life experience, job, and daily personal care. Irritation agents such as strong acidic compounds or alkali change the important parameters of skin such as the transepidermal water loss, erythema, and the level of stratum corneum hydration. Figure 6 presents the evolution of TWL.

Figure 6 Evolution of TWL during the experiment.

In skin evaluations, TWL has increased significantly in the case of pure GE during the 3-week experiment, and this increase can be associated with an irritation effect. The skin patches with hot peppers are well known to relieve the rheumatic pain, and, on the other hand, the irritation character of spicy herbs is known too. TWL evolution presents lower increases in the case of GE entrapped inside PU nanoparticles; thus, we can appreciate that this carrier is proper due to its lower irritation effect.

The skin pigmentation is mainly based on the level of melanin. People with Fitzpatrick Skin Phototype I always suffer skin burns and not tan, while those with Phototype V and VI always develop dark tan.24 CD1 Nu/Nu mice strain has a white and sensitive skin; this is the reason why small increases of melanin index were obtained during our experiment. The melanin scale used by Courage–Khazaka has 1,000 arbitrary units; thus, our changes (around 70 units/3 weeks) are insignificant (Figure 7).

Figure 7 Evolution of melanin during the experiment.

Erythema, probably the most important skin parameter, was another skin parameter evaluated in our experiment. Figure 8 presents an important increase of hemoglobin level in the case of mice treated with GE. This increase confirms the skin alteration that was suggested by the increase of TWL. The other samples, the PU nanoparticles with and without GE, present similar increases with the blank.

Figure 8 Evolution of erythema during the experiment.

Discussion

Nanotechnology is considered at this moment as an “emerging technology” that can revolutionize a large number of domains. In pharmaceutical technology, a research major priority is the development of alternative routes of drug administration.25 The appearance of nanomedicine and drug delivery systems, in particular, the polymeric carriers, is correlated with the advancement in understanding disease mechanisms and with the development of new polymer structures and synthetic methodologies for adapting its physicochemical properties. Although the drug delivery systems based on synthetic polymers have been used in drug delivery in recent decades, there are several examples of new macromolecules used in the field. It is important to explore these macromolecules and to persevere in the investigation of their production methods, cleaner, and more efficient and less costly although clinical approval of them may be strenuous. Nonetheless, clinical approval of them may be strenous.26

GE is often used due to its rich composition of volatile oils. In traditional Chinese, Indian, and Japanese medicine, it was used due to the heater effect, to increase digestive capacity, in bloating, nausea, vomiting, as well as in respiratory diseases characterized by the increase in cold quality. In Africa, the plant was introduced in the 15th century, being used by natives in cases of malaria and yellow fever. The first medical treatment based on ginger was written in the 13th century by Dr. Rhiwallow at the order of the Prince of South Wales. Ginger is currently grown in tropical countries around the world.27,28

Verma et al described that dried ginger powder has an important protective effect on cardiovascular disease by decreasing the lipid peroxidation and enhancing the fibrinolytic activity on experimentally induced atherosclerosis in rabbits.29 A treatment with just 1 g ginger powder has a synergetic effect on nifedipine and on antiplatelet aggregation in hypertensive patients, and a dose of 10 g powder exhibits a significant reduction of platelet aggregation in patients with coronary artery disease.30,31 On the other hand, the scientific literature presents that natural compounds from ginger have beneficial effects on blood pressure and they can be beneficial in lowering elevated blood glucose levels and lipid concentrations.3237

The ethanol extract suppresses the increase of body weight and decreases the levels of insulin and serum glucose induced by a fat diet.38 Unfortunately, this extract is contraindicated in gastritis and gastroduodenal ulcers due to the synergism effects of alcohol and its chemical compounds. The encapsulation of GE inside a drug delivery system with controlled release is a solution to this problem.

The molecular weight of polymers always presents a distribution with different dispersity. The polydispersity index (PDI) value is the ratio between the weight average molecular mass (Mw) and the number average molecular mass (Mn).39 Mn depends on the mass of PEG used as raw material and the dependence is almost linear; Ketata et al found by ESI/MALDI-TOF technique that Mn of a PU synthesized by PEG 200 is 230, while the Mn of a PU based on PEG 2000 is 2,030.40 Different PU nanoparticles have been obtained in this study in order to assure a prolonged release of GE; PDI between 0.4 and 0.7 indicates the obtaining of a carrier with an increased degree of heterogeneity.

The yield of encapsulation of a drug carrier is an important characteristic. The amount of loaded drug depends on the size of carrier particles. On the other hand, Biswal et al studied the influence of drug/polymer ratio on the encapsulation efficiency.41 They found that 1:1 and 1:2 ratios lead to medium encapsulation efficiencies (between 54% and 68%), while 1:5 and 1:6 ratios lead to the best encapsulation efficiency (around 95%). Hallow PU nanoparticles always present higher encapsulation efficiency than other polymer carriers such as dendrimers; we obtained satisfactory encapsulation efficiency equal with 82.9% in this research.

The evaluations of TWL and erythema show the nonirritation effects of GE samples entrapped inside PU nanoparticles.

Conclusion

In this experiment, PU nanoparticles with GE were obtained using a polyaddition process combined with a simultaneous emulsification. The products present an almost neutral pH and low solubility and are stable against thermal degradation. The transmembrane carrier contains particles with sizes around 90 nm, with a medium stability against the tendency to form clusters; they absorb at 300–310 nm. Good encapsulation efficacy (82.9%) and transmembrane penetration rate were obtained. The carrier with and without GE did not present irritation effects in in vivo testing on murine skin.

Acknowledgments

MALDI-TOF analysis was done in the Center of Genomic Medicine of “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania (POSCCE [Programul Operational Sectorial Cresterea Competitivitatii Economice (Sectoral Operational Program for the Increasing of Economic Competitiveness) Project ID: 1854, code SMIS [Sistemul Unic de Management al Informației pentru Instrumentele Structurale (Unique Information Management System for Structural Instruments): 48749, “Center of Genomic Medicine v2”, contract 677/09.04.2015).

Disclosure

The authors report no conflicts of interest in this work.

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