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Standardized Arrabidaea chica Extract Shows Cytoprotective Effects in Zoledronic Acid-Treated Fibroblasts and Osteoblasts

Authors Wiziack Zago PM, Oliveira Sousa IM, Servat-Medina L, Jorge MP, Lima Neto LG, Hass V, Li X, Tasca Gois Ruiz AL, Saxena D, Foglio MA

Received 29 April 2020

Accepted for publication 26 June 2020

Published 11 August 2020 Volume 2020:12 Pages 327—333


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Professor Christopher E. Okunseri

Patricia Maria Wiziack Zago,1 Ilza Maria Oliveira Sousa,2 Leila Servat-Medina,2 Michelle Pedroza Jorge,2 Lidio Gonçalves Lima Neto,3 Viviane Hass,4 Xin Li,5 Ana Lucia Tasca Gois Ruiz,2 Deepak Saxena,5 Mary Ann Foglio2

1Sao Leopoldo Mandic Medical School, Araras, São Paulo, Brazil; 2Faculty of Pharmaceutical Sciences at University of Campinas, Campinas, São Paulo, Brazil; 3Postgraduate Program in Parasitic Biology, CEUMA University, São Luis, Maranhão, Brazil; 4School of Dentistry, University of Missouri-Kansas City, Kansas City, MO, USA; 5College of Dentistry, New York University, New York, NY, USA

Correspondence: Mary Ann Foglio
Faculty of Pharmaceutical Sciences at University of Campinas, Rua Candido Portinari 200, CEP: 13083-871, Campinas, São Paulo, Brazil
Tel +55 19 3521-8132

Introduction: Osteonecrosis of the jaw is a condition associated with intraoral ulceration and bone necrosis induced by antiresorptive medications, such as zoledronic acid, a bisphosphonate. Previous data on Arrabidaea chica (H&B.) Verlot wound healing activity prompted the study reported herein on A. chica standardized hydro alcoholic extract in vitro cytoprotective activity data on epithelial and osteoblastic cells exposed to zoledronic acid (ZA).
Methods: Primary human gingival fibroblasts and murine pre-osteoblasts were treated with ZA 10 μM together with 5 or 10 μg.mL− 1 A. chica extract for 24h and 48 h. At both times, cells were submitted to viability assay and caspase 3/7 activation evaluation. Statistical analysis used one-way ANOVA and p=0.05.
Results: In cell viability assay, a drastic damage effect of ZA appeared after 48 h in both epithelial (55.8%) and pre-osteoblastic cells (39.7%). When treated with ZA in combination with A. chica extract, cells showed higher viability values: 74.1%– 82.3% for fibroblasts and 66% for pre-osteoblasts. Furthermore, the combined treatment presented lower caspase 3/7 activation in fibroblasts and pre-osteoblasts.
Conclusion: At low concentrations, A. chica extract showed promising cytoprotective effects against ZA-induced damage actions; however, further in vitro and in vivo studies are required to establish the mechanism of action.

Keywords: Arrabidaea chica, zoledronic acid, epithelial cells, osteoblasts, fibroblasts

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