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Role of ICAM-1 and E-selectin gene polymorphisms in pathogenesis of PAOD in Egyptian patients

Authors Shaker O , Zahra A, Sayed A, Refaat A, El-Khaiat Z, Hegazy G, El-Hindawi, Ay-El Deen

Published 24 December 2009 Volume 2010:6 Pages 9—15


Review by Single anonymous peer review

Peer reviewer comments 1

Olfat Shaker1, Amr Zahra2, Ahmed Sayed3, Ayman Refaat4, Zakaria El-Khaiat5, Gehan Hegazy5, Khaled El-Hindawi3, Mohamed Ay-El Deen3

1Department of Medical Biochemistry, 3Vascular Surgery, Faculty of Medicine, Cairo University, Cairo, Egypt; 2Department of Medical Biochemistry, Faculty of Medicine, Fayoum University, Al Fayyum, Egypt; 4Vasular Surgery, Faculty of Medicine, Beni Suef University, Beni-Suef, Egypt; 5Medical Biochemistry Department, National Research Center, Cairo, Egypt

Background: Intercellular adhesion molecule-1 (ICAM-1) and E-selectin have been shown to predict cardiovascular disease (CVD) such as myocardial infarction, stroke, and peripheral arterial occlusive disease (PAOD).

Methods: Two mutations, S128R in E-selectin and K469E in ICAM-1, were investigated in 156 patients with PAOD and 100 control subjects using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis in an Egyptian population.

Results: The distribution of E-selectin genotypes in patients affected by PAOD was 84.6% for the AA genotype and 15.4% for the AC genotype. In the control arm the distribution was 97% for the AA genotype and 3% for the AC genotype. There was a statistically significance difference in the distribution of the AC genotype in PAOD patients when compared with the control subjects. Additionally, the distribution of ICAM-1 genotypes in patients affected by PAOD was 30.8% with the EE, 48% with the EK, and 21.2% with the KK genotypes. The distribution of ICAM-1 genotypes in control subjects was 13% EE, 33% EK and 54% KK. The EE genotype was significantly more common in PAOD patients than in the controls.

Conclusion: S128R and K469E polymorphisms were associated with increased risk in PAOD. Early detection of these polymorphic genes helps in early prophylaxis against PAOD.

Keywords: polymorphism, PAOD, E-selectin, ICAM-1, RFLP, genotyping

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