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Repeated short climatic change affects the epidermal differentiation program and leads to matrix remodeling in a human organotypic skin model

Authors Boutrand LB, Thépot A, Muther C, Boher A, Robic J, Guéré C, Vié K, Damour O, Lamartine J

Received 27 August 2016

Accepted for publication 8 November 2016

Published 13 February 2017 Volume 2017:10 Pages 43—50


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Dr Jeffrey Weinberg

Laetitia-Barbollat Boutrand,1 Amélie Thépot,2 Charlotte Muther,3 Aurélie Boher,2 Julie Robic,4 Christelle Guéré,4 Katell Vié,4 Odile Damour,5 Jérôme Lamartine1,3

1Departement de Biologie, Université Claude Bernard Lyon I, 2LabSkinCreations, 3CNRS UMR5305, Laboratoire de Biologie Tissulaire et d’Ingénierie Thérapeutique (LBTI), Lyon, 4Laboratoires Clarins, Cergy-Pontoise, 5Banque de Tissus et Cellules, Hospices Civiles de Lyon, Lyon, France

Abstract: Human skin is subject to frequent changes in ambient temperature and humidity and needs to cope with these environmental modifications. To decipher the molecular response of human skin to repeated climatic change, a versatile model of skin equivalent subject to “hot–wet” (40°C, 80% relative humidity [RH]) or “cold–dry” (10°C, 40% RH) climatic stress repeated daily was used. To obtain an exhaustive view of the molecular mechanisms elicited by climatic change, large-scale gene expression DNA microarray analysis was performed and modulated function was determined by bioinformatic annotation. This analysis revealed several functions, including epidermal differentiation and extracellular matrix, impacted by repeated variations in climatic conditions. Some of these molecular changes were confirmed by histological examination and protein expression. Both treatments (hot–wet and cold–dry) reduced the expression of genes encoding collagens, laminin, and proteoglycans, suggesting a profound remodeling of the extracellular matrix. Strong induction of the entire family of late cornified envelope genes after cold–dry exposure, confirmed at protein level, was also observed. These changes correlated with an increase in epidermal differentiation markers such as corneodesmosin and a thickening of the stratum corneum, indicating possible implementation of defense mechanisms against dehydration. This study for the first time reveals the complex pattern of molecular response allowing adaption of human skin to repeated change in its climatic environment.

Keywords: skin, organotypic tissue, climatic changes, transcriptome, collagen

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