Back to Journals » Clinical Ophthalmology » Volume 10

Rapid alkaline methylene blue supravital staining for assessment of anterior segment infections

Authors Kiuchi K

Received 6 July 2016

Accepted for publication 6 September 2016

Published 11 October 2016 Volume 2016:10 Pages 1971—1975

DOI https://doi.org/10.2147/OPTH.S116616

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Lucy Goodman

Peer reviewer comments 2

Editor who approved publication: Dr Scott Fraser


Katsuji Kiuchi

Kiuchi Eye Clinic, Joto-ku, Osaka, Japan

Purpose: To present the Löffler’s alkaline methylene blue technique of staining eye discharges in eyes with anterior segment infections.
Method: The Löffler’s alkaline methylene blue staining method is a simple staining technique that can be used to differentiate bacterial, viral, and fungal infections. It is a cationic dye that stains cells blue because the positively charged dye is attracted to negatively charged particles such as polyphosphates, DNAs, and RNAs. Specimens collected from patients by swabbing are smeared onto microscope slides and the methylene blue solution is dropped on the slide. The slide is covered with a glass cover slip and examined under a microscope. The entire time from the collection to the viewing is about 30 seconds.
Results: Histopathological images of the conjunctival epithelial cells and neutrophils in eye discharges were dyed blue and the nuclei were stained more intensely blue. Bacterial infections consisted mainly of neutrophils, and viral infections consisted mainly of lymphocytes.
Conclusions: Löffler’s alkaline methylene blue staining can be done in about 30 seconds for diagnosis. Even though this is a one color stain, it is possible to infer the cause of the infection by detection of the absence of bacteria and/or fungi in context of the differential distribution of neutrophils and lymphocytes.

Keywords: alkaline methylene blue, supravital staining, discharge, anterior segment infections

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]