Back to Journals » International Journal of Nanomedicine » Volume 13

Potential effects of different natural antioxidants on inflammatory damage and oxidative-mediated hepatotoxicity induced by gold nanoparticles

Authors Abdelhalim MAK, Moussa SAA, Qaid HAY, Al-Ayed MS

Received 22 April 2018

Accepted for publication 30 August 2018

Published 23 November 2018 Volume 2018:13 Pages 7931—7938


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Thomas Webster

Mohamed Anwar K Abdelhalim,1 Sherif A Abdelmottaleb Moussa,2,3 Huda AY Qaid,1 Mohammed Suliman Al-Ayed1

1Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Committee of Radiation and Environmental Pollution Protection, Department of Physics, College of Science, Al-Imam Mohammad Ibn Saud Islamic University, Riyadh, Saudi Arabia; 3Biophysics Group, Biochemistry Department, Genetic Engineering and Biotechnology Division, National Research Centre, Giza, Egypt

The objective of this study was to verify and confirm the oxidative-mediated hepatotoxicity, inflammatory liver damage, and oxidative stress induced by intraperitoneal administration of gold nanoparticles (GNPs) in vivo; characterize the effect of different natural antioxidants on these hazardous changes; and finally choose the most powerful antioxidant among these different natural antioxidants.
Methods: Ten-nanometer GNPs were dissolved in aqueous solution of 0.01% concentration. A dose of 50 µL of 10 nm GNPs was administered intraperitoneally for 7 days to the rats, whereas the antioxidants were orally administered for the same time period. The antioxidants used in the study were vitamin E (Vit E), α-lipoic acid (ALA), quercetin (Qur), arginine (Arg), and melanin. Forty Wistar-Kyoto male rats were used. Rats were arbitrarily divided into seven groups after acclimatization for 1 week. For serum separation, blood samples were obtained from each animal. Serum liver function markers and tissue oxidative stress and lipid proxidation biomarkers were assessed.
Results: The increase in the levels of gamma-glutamyl transferase, alkaline phosphatase, total protein, alanine aminotransferase, and total bilirubin in the serum of rats and the increase of malondialdehyde in the hepatic tissue and decrease in reduced glutathione when compared with the control in this study confirmed the ability of GNPs to cause hazardous effects.
Conclusion: Treatment of rats with Vit E, ALA, Qur, Arg, and melanin along with GNPs significantly inhibited the inflammatory liver damage, lipid peroxidation, and the oxidative stress induced by GNPs in vivo, but with different responses due to their evaluated normalization values, and it has been confirmed that melanin is the most powerful antioxidant among these different natural antioxidants, ie, it has the most effective potential role against the hepatic inflammatory damage, oxidative stress, and lipid peroxidation.

Keywords: antioxidants, oxidative stress, gold nanoparticles, hepatotoxicity, vitamin E, α-lipoic acid, quercetin, arginine, melanin, rats

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]