Peptide crosslinked micelles: a new strategy for the design and synthesis of peptide vaccines
Authors Jihua Hao, Marcin Kwissa, Bali Pulendran, Niren Murthy
Published 15 March 2006 Volume 2006:1(1) Pages 97—103
Jihua Hao1, Marcin Kwissa2, Bali Pulendran2, Niren Murthy1
1Georgia Institute of Technology, Department of Biomedical Engineering, Atlanta, GA, USA; 2Emory Vaccine Center, Department of Pathology, Atlanta, GA, USA
Abstract: This report presents a new and simple methodology for the synthesis of multicomponent peptide vaccines, named the peptide crosslinked micelles (PCMs). The PCMs are core shell micelles designed to deliver peptide antigens and immunostimulatory DNA to antigen-presenting cells (APCs). They are composed of immunostimulatory DNA, peptide antigen, and a thiopyridal derived poly(ethylene glycol)-polylysine block copolymer. The peptide antigen acts as a crosslinker in the PCM strategy, which allows the peptide antigen to be efficiently encapsulated into the PCMs and also stabilizes them against degradation by serum components. Cell culture studies demonstrated that the PCMs greatly enhance the uptake of peptide antigens into human dendritic cells.
Keywords: composite, crosslinking, peptide, vaccine