Nutritional support and dietary interventions for women with polycystic ovary syndrome
Authors Papavasiliou K, Papakonstantinou E
Received 1 June 2017
Accepted for publication 21 July 2017
Published 6 September 2017 Volume 2017:9 Pages 63—85
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 3
Editor who approved publication: Dr Chandrika Piyathilake
Kleopatra Papavasiliou, Emilia Papakonstantinou
Unit of Human Nutrition, Department of Food Science and Human Nutrition, Agricultural University of Athens, Athens, Greece
Abstract: Polycystic ovary syndrome (PCOS) is a common endocrine disorder in reproductive-aged women, which leads to reproductive, metabolic and hormonal abnormalities. Hyperinsulinemia, insulin resistance, androgen excess, ovulatory dysfunction, polycystic ovaries, gonadotropin abnormalities, obesity, adipose tissue dysfunction, difficulty to conceive and high-risk pregnancy are the most common PCOS-associated complications. The aim of this review was to describe and evaluate the effects of dietary interventions on PCOS-associated outcomes and to provide some evidence-based dietary advice for use in clinical practice. There is no optimal diet or macronutrient composition for PCOS. However, lifestyle modification, including a small-to-moderate weight loss of 5–10% (combined diet with regular physical activity) with any dietary pattern of choice, depending on the individuals’ preferences, culture, habits and metabolic needs (ie, Mediterranean diet, Dietary Approaches to Stop Hypertension [DASH] diet or moderately low-carbohydrate diets [30–45% of energy]), as well as alternative dietary interventions, including small, frequent meal (five to six meals daily) consumption at regular times, with the majority of carbohydrates consumed at lunch time or equally distributed throughout the day, seems to offer the evidence-based first-line strategy for the management of PCOS symptoms and insulin resistance. No conclusions can be drawn at this time for high protein diets, polyunsaturated fatty acids or micronutrient supplementation.
Keywords: nutrition, meal frequency, dietary strategies, insulin resistance
Polycystic ovary syndrome (PCOS) is a complex endocrine disorder in reproductive-aged women with a prevalence from 4% up to 18%, is the most common cause of infertility and is associated with reproductive, metabolic and hormonal dysfunction and higher association of pregnancy complications.1–8 Women with PCOS with menstrual irregularities, related to anovulation, may have difficulties to conceive.5 The majority of women with PCOS have hyperinsulinemia and insulin resistance (IR), which have a significant role in the pathogenesis of the syndrome and in the long run may lead to impairment of glucose metabolism, androgen excess with hyperandrogenic symptoms, gonadotropin abnormalities, ovulatory dysfunction and polycystic ovaries, higher intra-abdominal fat and adipose tissue dysfunction, independently of body mass index (BMI).5,9–18
Women with PCOS usually weigh more than women without PCOS, and many studies show that more than half of women with PCOS are found to be obese and tend to gain more weight longitudinally compared to women without PCOS.5,19–22 However, despite the fact that the majority of women with PCOS are overweight or obese, many lean women with PCOS are also considered to be at increased risk for metabolic disturbances.23,24 Central obesity, race and age are positively associated with IR.14,25,26 Central obesity and excess body fat exacerbate IR, dyslipidemia and hormonal dysfunction.5,26–29 Moreover, women with PCOS have increased risk of metabolic syndrome, gestational diabetes, type 2 diabetes and cardiovascular diseases.8,22,26,29,31–33
The main cause of infertility in women with PCOS is anovulation, whereas obesity seems to exacerbate symptoms and reduces conceiving rates.5,30 Some of the mechanisms involved in infertility may be ovarian hyperandrogenism, including luteinizing hormone (LH) hypersecretion, relative follicle-stimulating hormone (FSH) insufficiency, hyperinsulinemia and high anti-Mullerian hormone (AMH) inhibiting aromatase activity.5 Some adipokines secreted by adipose tissue may also be involved in obesity-mediated infertility, including low levels of adiponectin, related to androgen excess, and increased visfatin levels, related to glucose dysmetabolism.5,16 Leptin, resistin and retinol-binding protein 4 do not seem to play a significant role in PCOS with obesity-related infecundity.5,16 However, leptin is positively associated with BMI and LH, but not insulin, in women with PCOS.34
Diet and its effect on metabolic outcomes should be more thoroughly examined in women with PCOS. Women with PCOS seem to have a greater appetite, consume more energy-dense high glycemic index (GI) foods and saturated fat, have inadequate fiber intake and have low scores for PCOS-related quality of life, although their overall energy intake, physical activity and resting metabolic rate are similar to controls.21,35–37
Reduction in IR has been suggested as the principal goal of PCOS treatment. Lifestyle changes (diet plus physical activity), along with weight loss (5–10%), are proposed as the first-line strategy for amelioration of IR, ovulatory function and decreased free testosterone levels in women with PCOS.38–45 Trunk fat, waist circumference (WC) and BMI are the best predictors of IR in PCOS.46 Other dietary interventions, including carbohydrate distribution, meal frequency and timing, adequate intake of n-3 fatty acids and/or vitamin D supplementation, have been suggested to offer some additional benefits for markers of glucose and energy metabolism and reproductive hormonal regulation. In this review, we discuss nutritional support and dietary interventions for women with PCOS and attempt to derive to some evidence based conclusions for use in clinical practice. Tables 1 and 2 summarize the dietary interventions for women with PCOS.
Options for PCOS treatment
Typical treatment modalities used in women with PCOS include lifestyle modification (LSM; diet, physical activity and cognitive behavior therapy) and pharmacotherapy, such as clomiphene citrate (CC), aromatase inhibitors, low-dose human menopausal gonadotropin (hMG) or FSH, insulin sensitizers, laparoscopic ovarian drilling and in vitro fertilization (IVF).5,47–50 CC is the first-line therapy for ovulation induction in anovulatory infertile women with PCOS, although almost 20% of patients are unresponsive.47,51 Results from a three-arm randomized, parallel, controlled, assessor-blinded clinical trial investigating the effects of a 6-week intervention with structured exercise training and hypocaloric diet on probability of ovulation after CC in overweight and obese CC-resistant PCOS women showed that structured exercise training combined with hypocaloric diet was effective in increasing the response to CC and the ovulation rate in overweight and obese PCOS patients.52 Results from a study comparing immediate treatment with CC to delayed treatment with CC after preconception treatment with continuous oral contraceptives, LSM (including caloric restriction, antiobesity medication, behavioral modification and exercise) or the combination of both showed benefit of improved ovulation and live birth with delayed infertility treatment with CC when preceded by LSM with weight loss compared to immediate treatment.53 Moreover, a preconception lifestyle intervention leading to 6–7% weight loss eliminated the adverse metabolic oral contraceptive effects and led to higher ovulation rates.54
Aromatase inhibitors, such as letrozole, are also used for ovulation induction.47 A recent Cochrane review showed that letrozole appeared to improve live birth and pregnancy rates in subfertile women with anovulatory PCOS, compared to CC.55 There seems to be no difference in effectiveness between letrozole and laparoscopic ovarian drilling.55 A recent meta-analysis investigating the comparative efficacies of ovulation induction treatments of 26 randomized clinical trials with 2722 participants and nine types of therapies (CC, metformin, letrozole, FSH, hMG, unilateral laparoscopic ovarian drilling, bilateral laparoscopic ovarian drilling, the combination of metformin with letrozole and the combination of metformin with CC) showed that FSH, hMG and the combination of metformin with letrozole were potentially more effective therapies in improving reproductive outcomes compared to the other therapies.56 Obesity seems to affect the responsiveness to hMG and FSH with increased dose needed, although this needs further investigation.5,47
Insulin sensitizers, such as metformin, are recommended for women with PCOS with impaired glucose tolerance.51 A meta-analysis of 12 randomized controlled trials (RCTs) with 608 women with PCOS showed that the combination of lifestyle intervention and metformin was associated with lower BMI and subcutaneous adipose tissue and improved menstruation compared to lifestyle and placebo over 6 months, without significant differences between metformin alone compared to lifestyle on body weight loss.57
IVF is a third-line treatment for PCOS indicated in those who have not responded to first- or second-line ovulation induction therapies or in those who require IVF for other indications and can be used with the safer gonadotropin-releasing hormone antagonist protocol and metformin as an adjunct, although no ideal protocol has been identified yet and no dietary associations with IVF have been reported.5,47,58 Obesity increases the risk of conception failure with IVF.5,47,58 Fertility in women with PCOS is maintained until the age of 38 years using IVF, but thereafter pregnancy rates decrease.59 Women with PCOS who conceive singleton pregnancies after IVF may be at increased risk of pregnancy complications and may require close antenatal monitoring.7,60 In conclusion, PCOS therapy should be individualized.50,60 There is a need for well-designed, long-term, with adequate sample size, studies to evaluate the effectiveness of pharmacological PCOS treatments, alone or in combination with dietary interventions to ameliorate PCOS symptoms and fertility outcomes.
LSM programs and PCOS
Lifestyle intervention (diet plus physical activity) leading to a small to moderate weight loss of about 5–10% is sufficient to significantly improve IR, restore ovulation and improve menstrual regularity and conception, as well as ameliorate hyperandrogenism, hirsutism and dyslipidemia.15,16,48,49,58,60–64 Results from a cross-sectional study showed that women with PCOS were more likely to follow a number of both healthy body weight management practices (balanced diet, reduced fat or sugar intake, adoption of a low-GI diet) as well as paradoxical nonhealthy attitudes (ie, increased smoking, use of laxative and diet pills) compared to women without PCOS.65
Although most clinicians are in favor of lifestyle changes for the management of PCOS, the majority of women with PCOS reported that they rarely receive lifestyle advice from their therapists.66,67 LSM programs combine dietary recommendations aiming at a small-to-moderate weight loss or maintenance, physical activity patterns and behavioral and cognitive approaches. Several studies have shown that LSM programs may prevent or delay the onset of type 2 diabetes in people with impaired glucose tolerance and women with PCOS.15,68,69 LSM programs, including diet and exercise, with or without dedicated stress reduction techniques, may offer significant benefits for amelioration in anthropometric, reproductive (hyperandrogenism, menstrual function, ovulation, pregnancy, conception), metabolic (glucose metabolism, IR, dyslipidemia) and quality of life parameters.38,48,49
Results from five meta-analyses of RCTs with women with PCOS supported the benefits of LSM programs on decreased BMI, fasting blood glucose, IR, FSH, sex hormone-binding globulin (SHBG), total testosterone, androstenedione, free androgen index (FAI) and Ferriman–Gallwey (FG) score and improved cardiorespiratory fitness, but not on glucose tolerance or lipid profiles.57,63,70–72 No studies have assessed the effects of LSM programs on clinical reproductive outcomes, quality of life or treatment satisfaction in women with PCOS.2,70 A 1-year randomized, placebo-controlled trial compared the effects of four interventions: 1) metformin 850 mg two times daily, 2) LSM plus metformin 850 mg two times daily, 3) LSM plus placebo and 4) placebo group on anthropometric and hormonal parameters, and showed that the combined LSM plus metformin group offered greater changes in body weight (–9 kg), total testosterone (–27 ng/dL) and FAI (–9).73 Results from a study investigating the effects of a 3-month LSM in overweight/obese women with PCOS with anovulation, hyperandrogenism and polycystic ovaries compared to BMI-matched regularly menstruating controls, normal weight PCOS and normal weight controls showed that women with PCOS exhibited lower levels of insulin receptor substrate-1 and glucose transporter-4 (GLUT4) mRNA in their proliferative endometrium compared to BMI-matched controls, whereas LSM led to decreased body weight, improved menstrual regularity and increased endometrial insulin sensitivity.61
In conclusion, LSM (diet plus regular physical activity) should be the first-line strategy for the management of PCOS. The main challenge remains the long-term maintenance of healthy dietary attitudes and insulin sensitivity. A team-based approach along with lifestyle interventions may be more suitable for the management and treatment of PCOS.49,58,74,75
Negative energy balance and PCOS
Several studies assessing the effects of caloric restriction along with modified dietary macronutrient composition on multiple health outcomes and biochemical indices have shown that there is no optimal dietary macronutrient composition or dietary pattern for PCOS.38,41,76 Overall, there is little variation in weight loss with different diets, and this variation may be due to the differences in compliance and not how the body handles different macro- or micronutrients. Negative energy balance (with a deficit of 350–1000 kcal/day) seems to be the key factor leading to successful body weight and fat loss and amelioration of menstrual cycle and insulin sensitivity, irrespectively of the adopted dietary pattern.42,77–82
Results of a 1-month trial comparing the effects of two hypocaloric diets, differing in macronutrients, on a variety of clinical measures in women with PCOS, showed that negative energy balance alone resulted in significant weight loss (–4 kg) and decreased testosterone (–9 ng/dL), fasting insulin (–5 mIU/L), area under the curve (AUC) for insulin (–5.823 mIU/L⋅min), fasting leptin (–11 ng/mL), AUC for leptin (–1.854 ng/mL⋅min), total cholesterol (–22 mg/dL) and low-density lipoprotein (LDL) cholesterol (–12 mg/dL), independently of macronutrient composition of the tested diets.42 Similarly, results from another trial comparing the effects of two hypocaloric diets with different macronutrient composition and GI in women with PCOS showed that negative energy balance resulted in successful weight loss (–4%), decreased dehydroepiandrosterone sulfate (DHEAS) and increased SHBG concentrations.83 Moreover, results from a 6-month study comparing the effects of two hypocaloric diets (energy deficit –500 kcal/day), differing in macronutrient composition, showed that negative energy balance per se resulted in body weight and fat loss, amelioration of menstrual dysfunction (more regular menstrual episodes reported after weight loss) and hirsutism.77
In conclusion, negative energy balance is a key strategy for the management and treatment of PCOS. The size of the caloric deficit should be determined according to the individuals’ needs (dietary preferences, habits, culture and metabolic goals) and physical activity patterns.
Dietary carbohydrates and PCOS
The “low-carbohydrate diet” is typically defined as having <20% carbohydrates (equivalent to 20–60 g carbohydrates/day).84 Out of 12 studies reporting effects of low-carbohydrate diets, only one, nonrandomized 24-week trial with small sample size met the criteria of the low-carbohydrate definition with PCOS subjects instructed to limit carbohydrate intake to ≤20 g/day.85 One trial included a diet with <30% carbohydrates, six trials included diets with 40% carbohydrates and four studies with 41–45% carbohydrates.41,42,76,83,86–92
Douglas et al92 compared the effects of three eucaloric diets: 1) standard (16% protein, 56% carbohydrate, 31% fat), 2) moderately low-carbohydrate (15% protein, 43% carbohydrate, 45% fat) and 3) high monounsaturated fatty acid (MUFA) diets (15% protein, 55% carbohydrate, 33% fat) on glucose and insulin responses in women with PCOS and reported greater reductions in fasting insulin (–3 mIU/L) and lower acute insulin response to glucose (–98 mIU/L × 10 minutes) with the moderately low-carbohydrate diet compared to other two diets. In contrast, results from two clinical trials reported no significant differences in glucose or energy metabolism with moderately low-carbohydrate diets.41,42
Carbohydrate distribution may be a significant component for glucose metabolism and IR. One study provided evidence showing that the consumption of the majority of carbohydrates (50%) at lunch time resulted in the lowest postprandial glucose spikes and improved glycemic control, compared to the majority of carbohydrates consumed at breakfast, dinner or equally distributed throughout the day, in people with type 2 diabetes.93 Moreover, another study showed that consumption of a high-carbohydrate breakfast (>45% of energy from carbohydrates) may have detrimental effects in people with impaired glucose regulation and it should be avoided.94
In conclusion, there is no optimum amount of carbohydrate intake for women with PCOS, and, therefore, any range of dietary carbohydrates may be adopted, according to the individuals’ dietary assessment, metabolic goals, dietary habits and preferences. However, it may be advantageous to consume the majority of carbohydrates at lunch time, with the second best option, ie, their equal distribution in meals throughout the day, and to avoid a high-carbohydrate breakfast.
GI, glycemic load (GL) and PCOS
Five available clinical trials examined the effects of low GI on glucose or energy metabolism and hormonal responses in women with PCOS and reported successful weight loss, irrespective of GI.77,79,83,86,95 Out of the five trials, two showed increased insulin sensitivity79,95 and one showed decreased insulin levels, IR, DHEAS and high-sensitivity C-reactive protein (hsCRP) with adoption of low-GI diet.83
Results from one trial showed that a high-protein, moderately low-carbohydrate with low and medium GL resulted in significant reductions in blood insulin (–4 mIU/L), IR (homeostatic model assessment for IR [HOMA-IR], –0.8) and hsCRP (–0.9 mg/L) levels compared to a conventional diet.83 Similarly, results from a 12-month dietary intervention with two similar macronutrient composition, energy-reduced, low-fat, low-saturated fat, moderate-to-high fiber diets (23% protein, 50% carbohydrates, 27% fat), low or high GI, showed that the low-GI diet provided a threefold greater improvement in whole-body insulin sensitivity and improved menstrual regularity and better emotional scores, compared to a conventional hypocaloric, low-fat diet.79 However, both the low-GI and the conventional diets led to similar improvements or changes in blood lipids and androgenic hormone concentrations, markers of inflammation and other measures of quality of life.79 Wong et al86 showed that a low-GI diet led to a greater decrease in BMI percentile in adolescents with PCOS. In a nonrandomized clinical trial, participants replaced high-GI and medium-GI with low-GI foods, and findings showed possible improvements in metabolic risk factors (ie, insulin sensitivity).95
In conclusion, results from a few available studies suggest that consumption of low-GI foods may have a small additional beneficial impact on some outcome measures in women with PCOS.
Dietary protein and PCOS
Out of seven available trials comparing the effects of high-protein hypocaloric diets compared to low-protein diets on several outcomes in women with PCOS, four found no significant effects of dietary protein on indices of energy metabolism or androgenic parameters,42,80,85,96 two reported greater weight loss with higher protein intake87,89 and three reported greater reductions in body fat with higher protein diets.87–89 Only one trial reported greater reductions in glucose levels with higher protein diets.87
Some studies have attempted to separate the effects of high-protein intake (from diet alone or supplementation) from low-carbohydrate intake (diets typically with ≥20% protein, ≤45% carbohydrates, varying in fat content). Two studies found greater improvements in anthropometric and other outcome measures with high-protein diets,87,89 whereas three found no differences between high- and low-protein diets.41,42,88 Sørensen et al87 demonstrated that a high-protein, low-carbohydrate diet (~40% protein, 30% carbohydrate, 30% fat) decreased significantly more body weight (–4.4 kg), fat mass (–4.3 kg), WC (–3.7 cm) and glucose concentrations (–0.2 mmol/L) compared to a low-protein, high-carbohydrate diet (~15% protein, 55% carbohydrate, 30% fat). However, no differences between diets were found for SHBG, total and free testosterone and C-peptide.87 Toscani et al88 compared the effects of two diets, a high-protein, moderately low-carbohydrate diet (30% protein, 40% carbohydrate, 30% fat) or a low-protein, high-carbohydrate (15% protein, 55% carbohydrate, 30% fat) diet, and reported equal reductions in body weight, BMI, WC, body fat and the sum of trunk skinfolds with both diets. Similarly, results from another study comparing the effects of a high-protein, moderately low-carbohydrate (30% protein, 40% carbohydrate, 30% fat) vs a low-protein, high-carbohydrate diet (15% protein, 55% carbohydrate, 30% fat) showed successful weight loss and improvement in reproductive and metabolic abnormalities with both diets.41
Results from one trial comparing the effects of a high whey protein, moderately low-carbohydrate diet vs normal protein, high simple carbohydrate diet on glucose metabolism, body composition, blood lipids and indices of inflammation in patients with PCOS, using two types of hypocaloric diets, a high-protein, moderately low-carbohydrate (~34% protein, 40% carbohydrate, 26% fat) or a low-protein, high simple carbohydrate diet (~17% protein, 57% carbohydrate, 26% fat), showed greater weight and fat mass loss with the high whey protein, moderately low-carbohydrate diet.89 However, no differences were found for fasting glucose, insulin, IR, glycated hemoglobin A1c (HbA1c), triglycerides (TGs) or hsCRP between diets.89 A meta-analysis of RCTs suggested that high-protein, moderately low-carbohydrate diets (within 6 months) may have some beneficial effects on weight loss, HbA1c and blood pressure in patients with impaired glucose metabolism.97 Moreover, our group has shown that, in obese people with and without type 2 diabetes, dietary protein did not have an important effect on glucose metabolism,98 but others have shown that it increases insulin response.99 Some proposed mechanisms for the beneficial effects of higher protein diets on body composition and weight loss include increased protein-induced thermogenesis100,101 and increased satiety, possibly due to enhanced cholecystokinin production.102,103 Moreover, a meta-analysis of RCTs reported that the majority of RCTs (35 out of 51) demonstrated a benefit of fat-free mass preservation with higher protein diets.104
In conclusion, although some studies support that higher protein diets may result in several positive health outcomes, including lean mass preservation during weight loss and maintenance, better glycemic control and amelioration of other cardiovascular disease risk factors, such as blood pressure, it is not clear yet whether these effects are due to the higher protein or lower carbohydrate intake, and no recommendations can be made currently. Nevertheless, the addition of 7–15 g of dietary protein in meals and snacks may offer some additional health benefits for women with PCOS, including amelioration of insulin sensitivity and lower postprandial glucose fluctuations, but this still needs to be confirmed.
Dietary fat and PCOS
Eight clinical trials compared the effects of hypocaloric low- vs high-fat diets, typically using diets with a mean of 20–30% energy from fat or dietary patterns (ie, Dietary Approaches to Stop Hypertension [DASH]), on energy metabolism in women with PCOS, and all reported significant reductions in body weight and composition with adoption of low-fat diets.76,78,90–92,105,106,152
Moderately low-carbohydrate, high-fat diets have been shown to decrease fasting insulin and AUC for insulin and to increase insulin sensitivity in three trials in women with PCOS.90–92 In one trial, a high-fat, moderately low-carbohydrate eucaloric diet resulted in greater reductions in body fat, intra-abdominal adipose tissue, subcutaneous abdominal adipose tissue and intermuscular adipose tissue compared to a control diet.76 Results from another trial comparing two isocaloric diets – a low-fat, low-saturated fat, high-carbohydrate (~15% protein, 60% carbohydrate, 25% fat, 7% saturated fat) or a high-fat, low-saturated fat, moderately low-carbohydrate diet (~15% protein, 40% carbohydrate, 45% fat, 7% saturated fat) – showed a 30% greater reduction in AUC for insulin and blood lipids with the high-fat, low-saturated fat, moderately low-carbohydrate diet, in the absence of weight loss.90 Results from a short-term, crossover trial showed that a moderately low-carbohydrate (43%), high unsaturated fatty acids (monounsaturated fatty acids, MUFA: 18%; polyunsaturated fatty acids, PUFA: 17%) diet, led to a greater decrease in fasting insulin concentrations and the acute insulin to glucose response compared to the control diet.92 Results from another crossover clinical trial showed that a high-fat and moderately low-carbohydrate (~41% carbohydrate, 19% protein and 40% fat) diet induced significant glucose metabolism improvements (decreased basal β-cell response, fasting insulin, fasting glucose and IR), hormonal responses (lower testosterone) and decreased blood lipids and adipose tissue (intra-abdominal adipose tissue [–7.1%], subcutaneous abdominal adipose tissue [–4.6%] and intermuscular adipose tissue [–11.5%]).76,91
DASH diet is a low-GI, low-energy-density, high in complex carbohydrates, high fiber diet (~18% protein, 52% carbohydrate, 30% fat), which has been shown to produce significantly lower blood pressure and glycemic responses.99,107 Asemi and Esmaillzadeh78 demonstrated that adherence to DASH eating pattern, compared to a control diet based on Iranian traditional foods, resulted in greater reductions in body weight, BMI, WC, hip circumference (HC), serum insulin levels, IR, hsCRP and TG compared to the control diet in women with PCOS.106 Similarly, another trial showed that adherence to the DASH diet for 12 weeks had beneficial effects on weight loss, serum AMH, markers of insulin metabolism, SHBG, FAI, plasma nitric oxide and malondialdehyde levels among women with PCOS.105
In conclusion, replacement of dietary carbohydrate with MUFA and/or PUFA in a reduced energy diet may offer additional health benefits in the management of PCOS. The adoption of healthy dietary patterns such as DASH or the Mediterranean-style diets should be encouraged among women with PCOS, as they are rich in dietary fiber, antioxidants and anti-inflammatory nutrients, lead to greater satiety and have anti-hyperlipidemic, antihypertensive and antidiabetic properties.78,103,106
PUFA and PCOS
PUFAs, particularly the marine n-3 PUFA (eicosapentaenoic acid [EPA] and docosahexaenoic acid [DHA]), have been studied for their metabolic effects on insulin sensitivity, IR, androgen status and anti-inflammatory markers in women with PCOS.16,108–111 Mainly, most of the clinical trials of PUFA supplementation were of short duration (6–12 weeks) and the supplementation dose ranged between 2 and 4 g PUFAs per day.108–110,112–118 A recent meta-analysis of eight clinical trials with a total of 298 females with PCOS showed a slight reduction in serum total testosterone levels following n-3 PUFA supplementation among females with PCOS, without significant impact on SHBG and DHEAS levels.119 Results from a cross-sectional study showed that increased plasma n-6 PUFAs or increased n-6:n-3 PUFA ratio was associated with greater circulating androgen concentrations, whereas increased long-chain n-3 PUFA in plasma was associated with decreased blood lipids in women with PCOS.110 Subsequently, results from a crossover, placebo-controlled, RCT showed that long-chain n-3 PUFA supplementation (4×1 g capsules/day, containing 2.4 g total long-chain n-3 PUFAs, 1.9 g EPA and DHA/day in a ratio of EPA to DHA of 1.49:1) resulted in increased plasma concentrations of n-3 PUFAs and had an antiandrogenic effect in women with PCOS.110 Results from a double-blind RCT, providing n-3 PUFA supplementation (3 g/day) for 8 weeks, showed that n-3 PUFA led to a greater reduction in serum testosterone, but not SHBG, compared to the placebo group.112 Results from another double-blind RCT, providing n-3 PUFA supplementation (2 g/day) for 6 months in PCOS, showed that n-3 decreased lipid profiles, WC and interval between periods, with no changes in body weight, HC, fasting glucose, number of ovarian follicles, size of ovary, bleeding volume, menstrual bleeding and hirsutism score.120
Some have proposed that n-3 PUFAs may improve insulin sensitivity by decreasing the production of inflammatory cytokines including tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6) and increasing secretion of the anti-inflammatory hormone adiponectin.121 However, results from clinical trials on IR and insulin sensitivity remain controversial in women with PCOS. Nasri et al113 reported that 2 g n-3 PUFA supplementation from flaxseed oil (800 mg α-linolenic acid [ALA]/day) had beneficial effects on peroxisome proliferator-activated receptor gamma (PPAR-γ) and LDL receptor gene expression involved in the insulin and lipid signaling pathways. Similarly, Mohammadi et al114 showed that 4 g n-3 PUFA supplementation (720 mg EPA and 480 mg DHA) resulted in a 20% increase in adiponectin and decreased glucose (–11%) and insulin (–8%) concentrations. However, a recent meta-analysis with three available trials of small sample size reported no beneficial effects of n-3 PUFAs on IR or other clinical outcome measures in women with PCOS.121
In conclusion, although some studies have reported some beneficial health effects of PUFAS, particularly n-3 marine PUFAs, there is still a lot of controversy and no conclusions can be drawn at this point.
Meal frequency, meal timing and PCOS
Meal frequency and meal timing seem to be valuable components of lifestyle changes, although there are only limited data on women with PCOS. Some argue that frequent meal consumption is detrimental to body composition and indices of glycemic control, because it may lead to weight gain due to increased lipogenesis or fat deposition after meals or by simply increasing the overall energy intake.122–125 Furthermore, it has been suggested that increased meal frequency may increase postprandial glucose, insulin, IR and blood lipids and may have a negative impact on the fatty acid composition of serum phospholipids.126–129 In contrast, others propose that increased meal frequency may exert a beneficial effect on body weight and indices of glycemic control, which may be attributed to nutrient load spreading, producing lower postprandial insulin concentrations, hunger reduction, inhibitory effects of free fatty acids on glucose uptake suppression and increased glucose clearance from the circulation with a significant economy in insulin secretion.130,131
In a 24-week crossover RCT, our group reported a beneficial effect of a six-meal pattern, without energy restriction, on amelioration of post-oral glucose tolerance test (OGTT) insulin sensitivity and reduction in subjective hunger in lean and obese women with PCOS compared to a three-meal pattern.132 Another study by Jakubowicz et al133 compared two isocaloric diets (high-kilocalorie breakfast vs high-kilocalorie dinner) in lean women with PCOS and reported that high-energy intake at breakfast resulted in reduced food intake at dinner, which in turn led to improved insulin sensitivity and markers of reproductive function.
The timing of food intake has gained considerable interest in the past few years as it is found to affect metabolism and insulin secretion.134 It has been suggested that postprandial glycemia is under circadian regulation and that its misalignment may lead to glucose intolerance.130 Eating late during the day was associated with decreased resting-energy expenditure, decreased fasting carbohydrate oxidation, decreased glucose tolerance, blunted daily profile in free cortisol concentrations and decreased thermal effect of food on wrist temperature in normal weight, healthy females.135 People with prediabetes preferring to consume their main meal in the evening (later chronotype) had higher HbA1c compared to those consuming their main course at lunch time, and thus, had higher risk of developing type 2 diabetes sooner.136 The American Heart Association in their recent scientific statement concluded that meal frequency and timing may be important parameters in the nutrition management of chronic diseases, leading to healthier lifestyle and reduction in cardiometabolic risk factors.128
Vitamin D and PCOS
Epidemiological studies have shown inconsistent results for vitamin D status between women with and without PCOS.137 Some studies have demonstrated lower serum vitamin D concentrations in women with PCOS compared to women without PCOS, whereas a case–control study, with 85 women with PCOS and 115 healthy controls, found higher serum vitamin D concentrations in women with PCOS compared to controls.138–140 Low serum vitamin D levels or insufficiency has been positively associated with PCOS-related symptoms, such as central obesity, IR, infertility and hirsutism, whereas serum 25-hydroxy vitamin D (25OHD) seems to be an independent predictor of measures of reproductive success following ovulation induction.141–144
A recent systematic review and meta-analysis showed that vitamin D supplementation significantly decreased total testosterone, without any effects on serum SHBG and free testosterone.137 Another systematic review discussed the association between vitamin D status and metabolic disturbances and suggested an inverse association between vitamin D status and IR in women with PCOS.145 One study showed that 1000 mg/day calcium plus 50,000 IU/week vitamin D supplementation for 8 weeks among vitamin D-deficient women with PCOS had beneficial effects on serum insulin levels, IR, TG and very-LDL (VLDL) levels, without affecting fasting glucose and other lipid profiles.146 Another study showed that the combination of 1500 mg/day metformin plus 1000 mg/day calcium and 100,000 IU/month vitamin D supplementation for 6 months had positive effects on weight loss, follicle maturation, menstrual regularity and improvement in hyperandrogenism in infertile women with PCOS.147
In conclusion, few available studies have found some, but limited, benefits of vitamin D supplementation among women with PCOS, and it remains a controversial topic. Systematic reviews and meta-analyses support that currently no recommendations can be made about vitamin D supplementation for PCOS, due to conflicting study results, small sample sizes, lack of adjustments for confounders, use of different definitions for PCOS, use of different assays for serum 25OHD measurement, short trial duration and use of varying amounts of vitamin D supplementation.145
PCOS and micronutrients
Few available studies have examined the effects of micronutrient supplementation on PCOS symptoms and biochemical indices. One study showed that 220 mg zinc sulfate supplementation per day for 8 weeks among PCOS had beneficial effects on fasting glucose, insulin, IR and TG.148 It has been reported that 200 mg/day selenium supplementation for 8 weeks among PCOS had beneficial effects on fasting insulin, IR, insulin sensitivity, TG and VLDL levels, without affecting fasting glucose and other lipid profiles.149 Another study found that 200 mg/day chromium supplementation for 8 weeks among PCOS had favorable effects on fasting insulin, IR and insulin sensitivity.150 Moreover, 5 mg/day folate supplementation for 8 weeks among PCOS had beneficial effects on inflammatory factors and biomarkers of oxidative stress.151 In conclusion, there is insufficient evidence to recommend micronutrient supplements for women with PCOS at this time.
In summary, this review aimed to discuss the effects of nutritional support and dietary interventions on various clinical outcome measures in women with PCOS. The results indicate that there is still a great need for well-designed, long-term, with adequate sample size, clinical trials examining all aspects of overall nutrition (ie, patterns, habits and alternative nutrition interventions) and nutrient effects (ie, micronutrients) in women with PCOS. From the available current trial data, we can conclude that LSM, including a small-to-moderate weight loss of about 7% achieved with any dietary pattern of choice, depending on the individuals’ preferences, culture, habits and metabolic needs (ie, DASH diet, moderately low-carbohydrate diets [40–45% of energy] or substitution of carbohydrates for MUFA or PUFA in the diet), as well as alternative dietary interventions, including increased meal frequency and regular meal timing, with the majority of carbohydrates consumed at lunch time, seems to offer the evidence-based nutritional support strategy for IR and the management and treatment of PCOS.
The authors report no conflicts of interest in this work.
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