Back to Journals » International Journal of Nanomedicine » Volume 4

Nanonized black soybean enhances immune response in senescence-accelerated mice

Authors Chan Y, Wu C, Chan K, Lin Y, Liao J, Wang M, Chang Y, Jeng KC

Published 4 February 2009 Volume 2009:4 Pages 27—35


Review by Single-blind

Peer reviewer comments 2

Yin-Ching Chan1,6, Chia-Chuan Wu1,6, Kung-Chi Chan1, Yo-Giao Lin1, Jiunn-Wang Liao3, Ming-Fu Wang1, Yung-Ho Chang1, Kee-Ching Jeng2,4,5

1Departments of Food and Nutrition and 2Applied Mathematics, Providence University, Taichung; 3Graduate Institutes of Veterinary Pathology and 4Medical Technology, National Chung Hsing University, Taichung, Taiwan; 5Department of Education and Research, Taichung Veterans General Hospital, Taichung, Taiwan; 6These authors have contributed equally to this work

Abstract: Soy isoflavones may have applications in cancer prevention and anti-inflammation, therefore this study was conducted to investigate the effect of dietary supplementation with black soybean on the immune response in the senescence-accelerated-prone mice (SAMP8) and -resistant mice (SAMPR1, as controls). The mechanism of isoflavones was also investigated. Six-month-old male SAMP8 and SAMR1 mice were divided into the control groups and experimental groups supplemented with nanonized (Nano-soy) or microparticled (Micro-soy) black soybeans (n = 8/group), respectively for 12 weeks. Human peripheral blood mononuclear cells (PBMC) and murine splenocytes were stimulated with mitogens and cytokines were determined by reverse transcriptase-polymerase chain reaction and/or ELISA. The results showed that body weight, food intake, and relative weights of organs did not differ among the SAMP8 control and experimental groups. Isoflavone (daidzin and genistin) intake was higher in the Nano-soy group than the Micro-soy group. The lymphoproliferation and production of interleukin-2 (IL-2) and interferon-gamma (IFN-γ) in the Nano-soy group had a significantly higher (P<0.05) than those in the control and Micro-soy groups. The Nano-soy supplemented mice reached these cytokine levels similar to SAMR1 mice. This result was consistent with the in vitro data that daidzein (a metabolite of daidzin), at a concentration of 10 μM, increased IL-2, IL-4, and IFN-γ production from phytohemagglutinin-stimulated PBMC (P<0.05). However at higher concentrations (>50 μM), daidzein only reduced IL-10 and IFN-γ levels, whereas genistein reduced levels of the IL-2, IL-4, IL-10, IFN-γ mRNA and protein and these results suggest that the Nano-soy supplementation improved immune response in SAMP8 mice which may be attributable to higher daidzin content in the black soybean preparation.

Keywords: nanonized black soybean, cytokines, senescence accelerated mice, splenocytes, peripheral blood mononuclear cells

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF] 


Readers of this article also read:

Study on the prostate cancer-targeting mechanism of aptamer-modified nanoparticles and their potential anticancer effect in vivo

Wu X, Tai Z, Zhu Q, Fan W, Ding B, Zhang W, Zhang L, Yao C, Wang X, Ding X, Li Q, Li X, Liu G, Liu J, Gao S

International Journal of Nanomedicine 2014, 9:5431-5440

Published Date: 21 November 2014

Spinal cord demyelination combined with hyperhomocysteinemia: a case report

Hao MM, Zhang Y, Hou SX, Chen YL, Shi M, Zhao G, Deng YC

Neuropsychiatric Disease and Treatment 2014, 10:2057-2059

Published Date: 3 November 2014

A novel lipid-based nanomicelle of docetaxel: evaluation of antitumor activity and biodistribution

Ma M, Hao Y, Liu N, Yin Z, Wang L, Liang X, Zhang X

International Journal of Nanomedicine 2012, 7:3389-3398

Published Date: 4 July 2012

Feasibility of motivational interviewing delivered by a glaucoma educator to improve medication adherence

Paul F Cook, Robert W Bremer, AJ Ayala, et al

Clinical Ophthalmology 2010, 4:1091-1101

Published Date: 21 September 2010

Enhanced endothelial cell functions on rosette nanotube-coated titanium vascular stents

Eli Fine, Lijie Zhang, Hicham Fenniri, Thomas J Webster

International Journal of Nanomedicine 2009, 4:91-97

Published Date: 17 April 2009