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Nanoencapsulation of natural triterpenoid celastrol for prostate cancer treatment

Authors Sanna V, Chamcheu JC , Pala N, Mukhtar H, Sechi M, Siddiqui IA

Received 6 August 2015

Accepted for publication 26 September 2015

Published 30 October 2015 Volume 2015:10(1) Pages 6835—6846


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Dr Thomas J Webster

Vanna Sanna,1,2 Jean Christopher Chamcheu,3 Nicolino Pala,1 Hasan Mukhtar,3 Mario Sechi,1,2 Imtiaz Ahmad Siddiqui3

1Department of Chemistry and Pharmacy, University of Sassari, Sassari, Italy; 2Laboratory of Nanomedicine, University of Sassari, Sassari, Italy; 3Department of Dermatology, University of Wisconsin, Madison, WI, USA

Abstract: Celastrol (CL), a triterpenoid extracted from the Chinese herb Tripterygium wilfordii, has recently attracted interest for its potential antitumor effects. However, unfavorable physicochemical and pharmacokinetics properties such as low solubility, poor bioavailability, and systemic toxicity, are limiting its therapeutic application. In this context, the development of innovative nanocarriers can be useful to overcome these issues, and nanoencapsulation would represent a powerful strategy. In this study, we developed novel CL-loaded poly(ε-caprolactone) nanoparticles (NPs), and investigated their antiproliferative efficacy on prostate cancer cells. CL-NPs were prepared using a nanoprecipitation method and fully characterized by physicochemical techniques. The antiproliferative effects on LNCaP, DU-145, and PC3 cell lines of CL-NPs, compared to those of free CL at different concentrations (0.5, 1.0, and 2.0 µM), were investigated. Moreover, fluorescence microscopy was utilized to examine the cellular uptake of the nanosystems. Furthermore, to elucidate impact of nanoencapsulation on the mechanism of action, Western analyses were conducted to explore apoptosis, migration, proliferation, and angiogenesis alteration of prostate cancer cells. The results confirmed that CL-NPs inhibit proliferation dose dependently in all prostate cancer cells, with inhibitory concentration50 less than 2 µM. In particular, the NPs significantly increased cytotoxicity at lower/medium dose (0.5 and 1.0 µM) on DU145 and PC3 cell lines with respect to free CL, with modulation of apoptotic and cell cycle machinery proteins. To date, this represents the first report on the development of biocompatible polymeric NPs encapsulating CL. Our findings offer new perspectives for the exploitation of developed CL-NPs as suitable prototypes for prostate cancer treatment.

Keywords: celastrol, tripterine, nanoparticles, poly(ε-caprolactone), prostate cancer


Celastrol (CL), also known as tripterine, a major biologically active pentacyclic triterpenoid compound (Figure 1) isolated from the traditional Chinese medicinal herb Tripterygium wilfordii, has been used for centuries in the treatment of autoimmune disease,1 asthma,2 chronic inflammation,3 and neurodegenerative disease.4 In recent years, it has attracted interest for its potential antitumor effects against leukemia,5 glioma,6 breast,7,8 and prostate cancer cells.9

Figure 1 Chemical structure of celastrol.

Focusing on prostate cancer, CL has been proposed as a promising medicinal small molecule for hormone-refractory prostate neoplasia. Previously, Yang et al first reported that CL directly inhibits the chymotrypsin-like activity of proteasome in androgen-independent PC3 (androgen receptor [AR]-negative) and androgen-dependent LNCaP (AR-positive) prostate tumor cells, accompanied by suppression of AR protein expression, and induction of apoptosis.9 More recently, Dai et al demonstrated that CL suppresses androgen-independent prostate cancer proliferation, invasion, and angiogenesis by inducing the apoptotic machinery and attenuating constitutive NF-kB activity in androgen-independent prostate cancer both in vitro and in vivo.10 Despite its potential in cancer treatment, further therapeutic application of CL is affected by the poor water solubility, low oral bioavailability, and multiple side effects.11,12 Furthermore, harmful solvents such as dimethyl sulfoxide (DMSO) need to be used to solubilize the hydrophobic CL, and this limits its clinical use.13,14 In spite of this, to overcome these physicochemical and pharmacokinetic deficiencies, and to reduce the amount of the effective dose, the nanoencapsulation of CL can represent a useful strategy.15,16

To date, only two studies have explored the effectiveness of nanoencapsulated CL in the treatment of prostate cancer.14,17 In the first study, CL-loaded PEGylated liposomes displayed anticancer activity in VCaP cells, comparable to that of the free drug reconstituted in DMSO.14 Later, cell-penetrating peptide-coated CL-loaded nanostructured lipid carriers noticeably enhanced antitumor activity in vitro and in vivo in comparison with free tripterine.17 However, the CL encapsulation in clinically suitable polymeric nanosystems has not yet been investigated.

In our previous studies, the hydrophobic polyester poly-(ε-caprolactone) (PCL), as block copolymer or in combination with many biocompatible/biodegradable polymers, was successfully proposed for the formulation of nanoparticles (NPs) in the prevention18 and treatment19 of prostate cancer, as well as for the controlled release of white tea extract.20

The present work is aimed at investigating the encapsulation of CL in novel polymeric PCL NPs as potential prototypes for prostate cancer treatment. The NPs were prepared by a nanoprecipitation method, and characterized in terms of morphology, encapsulation efficiency, chemical properties, and in vitro release kinetics. Moreover, the cellular uptake was visualized by confocal laser scanning microscopy, and antiproliferative efficacy of NPs was evaluated by in vitro cytotoxicity assays toward a panel of hormone-sensitive and hormone-independent human prostate carcinoma cells. In order to elucidate if nanoencapsulation can modify/retain the mechanistic identity of free compound, Western blot analyses were assessed to test the efficacy of the treatments on apoptosis, migration, proliferation, and angiogenesis in human prostate cancer cells.

Materials and methods

Chemicals and reagents

CL was purchased from Baoji Guokang Bio-Technology Co., Ltd, (Shaan’xi Province, People’s Republic of China). PCL and Pluronic® F-127 were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). All solvents and other chemicals were obtained from Sigma-Aldrich Co., were analytical grade and were used without further purification. Primary antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA) or Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Anti-mouse and anti-rabbit secondary antibody horseradish peroxidase conjugates were obtained from Cell Signaling Technology. BCA (bicinchoninic acid) protein assay kit was obtained from Pierce (Rockford, IL, USA). Novex precast Tris-glycine gels were purchased from Bio-Rad Laboratories Inc. (Hercules, CA, USA).

Formulation of NPs

CL-loaded NPs were prepared by a nanoprecipitation method dissolving PCL (47.5 mg) and CL (2.5 mg) in 3 mL of acetonitrile, and adding the solution dropwise to 20 mL of Pluronic F-127 solution (1%, w/w) under gentle magnetic stirring. The resulting NPs’ suspension was evaporated under stirring (265.5× g) at room temperature to remove the organic solvent. NPs were centrifuged at 2655× g for 5 minutes and washed three times with water to remove the unencapsulated CL. Unloaded NPs were produced in a similar manner and used as comparison. Additionally, fluorescent fluorescein isothiocyanate-loaded NPs were prepared by adding 1.0 mg of FITC instead of CL, and used for the cellular uptake experiments.

Particle size and polydispersity index (PDI)

Mean diameter, size distribution, and PDI of the NPs were measured by photon correlation spectroscopy with Nanosizer S90 particle size analyzer (Malvern Instruments, Malvern, UK). The samples were diluted with deionized water and analyzed at 25°C. Each analysis was carried out in triplicate.

Zeta potential

Zeta potential of the NPs was measured at 25°C with a Zeta Plus analyzer (Brookhaven Instruments, Holtsville, NY, USA). The samples were diluted with distilled water and sonicated for several minutes before measurement. Data were obtained by the averaging of three measurements.

Scanning electron microscopy (SEM) analysis

Morphological examination of NPs was performed by SEM (model DSM 962; Carl Zeiss Meditec AG, Jena, Germany). A drop of NPs aqueous suspension was placed on aluminum stub and dried under vacuum for 12 hours. The samples were then analyzed at 20 kV acceleration voltage after gold sputtering, under an argon atmosphere.

Fourier transform infrared spectroscopy (FTIR)

The chemical composition of the CL raw material, unloaded and CL-loaded NPs was analyzed by FTIR spectral measurements by using a Nicolet Nexus FTIR spectrophotometer at a resolution of 4 cm−1 in KBr pellets, in the range 400–4,000 cm−1.

Raman spectroscopy

The chemical composition of the CL raw material, empty NPs, and CL-loaded NPs was analyzed by Raman spectroscopy (Senterra Raman microscope; Bruker Optik GmbH, Ettlingen, Germany) with a laser excitation of 532 nm and 5 mW nominal power and a 50× objective. The spectra were recorded in the 70–4,500 cm−1 range, with a resolution of 9 cm−1, an integration time of 3 seconds and 6 co-additions.

Thermal analyses

The thermal properties of the empty and CL-loaded NPs were determined using a differential scanning calorimeter (DSC) Q100 V 9.0 (TA Instruments, New Castle, DE, USA). Indium was used to calibrate the instrument. The thermograms of samples were obtained at a scanning rate of 20°C/min in 20°C–350°C temperature range and performed under an AR purge (50 mL/min). The thermal measurements were carried out on pure CL, freeze dried unloaded and CL-loaded NPs.

Drug loading content (DLC), encapsulation efficiency, and yield of production

To determine the drug loading, an aliquot of NPs (1 mg) was dissolved in 1.0 mL of acetonitrile. The solution was filtered through a 0.2 μm syringe filter and analyzed by ultraviolet-visible spectroscopy (Cary 3, Varian) at 422 nm. The amount of CL was calculated by referring to the calibration curve in acetonitrile (standard solutions in the range of 1.0–30 μg/mL; y=0.0341× −0.0009; R2=0.9999).

The DLC%, drug entrapment efficiency (EE%), and yield of production (YP %) of NPs were presented by the following equations, respectively:




In vitro release studies

Approximately 2.0 mg of CL-loaded NPs and pure CL were placed into dialysis bags and suspended in 20 mL of phosphate-buffered saline (PBS), pH 7.4, then incubated at 37°C and stirred at 106.2× g. At set time points, 1.0 mL of solution was withdrawn and replaced with fresh solution. Samples were filtered and CL concentration was assayed spectrophotometrically at 422 nm, thus calculated by referring to the respective calibration curve with standard solutions (PBS:ethanol 4:1, v/v) in the range of 1–50 μg/mL (y=0.0339× −0.0091; R2=0.9999).

Cell culture

The prostate carcinoma LNCaP, DU-145, and PC3 cells were obtained from American Type Culture Collection (Manassas, VA, USA). The cells were cultured in Roswell Park Memorial Institute 1640 and were maintained under standard cell culture conditions supplemented with 10% FBS and 1% penicillin/streptomycin at 37°C and 5% CO2 environment.

Cell growth and viability assay

The cell growth and viability were assessed by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay as previously described.21 Briefly, the cells were plated at 1×104 per well in 200 μL of complete culture medium. The next day, cells were treated with native CL or CL-loaded NPs for 24 hours, at 0.5, 1.0, and 2.0 μM equivalent CL concentrations. Each concentration was repeated in ten wells. After incubation for the specified time at 37°C in a humidified incubator, cell viability was determined. MTT (5 mg/mL in PBS) was added to each well and incubated for 2 hours, after which the plate was centrifuged at 500× g for 5 minutes at 4°C.

The MTT solution was aspirated from the wells using vacuum, and 0.2 mL of buffered DMSO was added to each well. After a 10-minute mixing, the absorbance was recorded on a microplate reader at the wavelength of 540 nm. The effect of each agent on growth inhibition was assessed as percentage of cell viability in which vehicle-treated cells were taken as 100% viable. The experiment was repeated three times with similar outcomes.

Cellular uptake

Fluorescence microscopy was utilized to examine the cellular uptake of the CL-NPs. Cells were plated in a two-chambered slide at 5,000 cells/well and allowed to grow for 18 hours. Post-attachment, the cells were incubated for 120 minutes with FITC-loaded NPs. Unbound micelles were removed by washing three times with PBS, the cells were mounted using Gold Antifade Reagent containing 4′,6-diamidino-2-phenylindole (Thermo Fisher Scientific, Waltham, MA, USA), and the mountant was allowed to cure overnight in the dark at room temperature. The images were examined under Nikon Eclipse Ti inverted microscope (Nikon Instruments, Melville, NY, USA), and then captured with a camera attached to the microscope. The experiment was repeated four times with similar results.

Protein extraction, quantification, and Western blotting

Prostate cancer PC3 cell lines were harvested after various treatments and whole cell lysates were prepared, resolved by sodium dodecyl sulfate denaturing and reducing polyacrylamide gel electrophoresis followed by Western blot analysis as described previously.22 Briefly, cells were washed with cold 1× PBS (10 mM, pH 7.4), incubated in ice-cold RIPA (radioimmunoprecipitation assay) lysis buffer freshly supplemented with Na3VO4 (100 mM), phenylmethanesulfonyl fluoride (1 mM) and protease inhibitor cocktail Set III (Calbiochem, La Jolla, CA, USA) on ice for 15 minutes. Cells were scraped into a microfuge tube, disaggregated through a 22 G needle, and lysates were cleared by centrifugation at 14,000× g for 25 minutes at 4°C, and the supernatants (lysate) were stored at −80°C until used. The concentration of the protein in lysates was determined by the BCA protein assay kit as per the manufacturer’s protocol (Thermo Fisher Scientific), and Western blot analysis was performed as described elsewhere.23 Equal quantities of protein 30–40 μg were electrophoresed on polyacrylamide gel electrophoresis, transferred onto nitrocellulose membranes, blocked with 5% non-fat dry milk in phosphate-buffered saline Tween 20, followed by overnight incubation at 4°C, with respective primary antibodies. After several washes, blots were incubated for 1 hour at room temperature with appropriate horse radish peroxidase-conjugated secondary antibodies, and binding was visualized by chemiluminescent detection system using enhanced chemiluminescence SuperSignal West Pico kit, and developed by the BioRad ultraviolet-trans camera or on film. Each blot was stripped and re-probed with β-actin as housekeeping assessing equal protein loading, and data are presented as the relative density of protein bands normalized to β-actin.

Cell migration

Cell migration was performed by using Radius™ 24-Well Cell Migration Assay kit from Cell Biolabs Inc. (San Diego, CA, USA) by following manufacturer’s instructions.

Statistical analysis

All data were subjected to one-way analysis of variance (GraphPadPrism, version 5.03). Individual differences were evaluated using a nonparametric post hoc test (Tukey’s test) and considered statistically significant at P<0.05.

Results and discussion

Nanotechnology-based strategies are an emerging and promising approach with a great potential to facilitate the chemoprevention and treatment of prostate cancer.2432 Meanwhile, the natural products are valuable sources of bioactive compounds that have drawn a great deal of attention because of their ability to suppress cancers as well as to reduce the risk of cancer development.3339 Potential advantages of NP-based therapeutic products are the capability to revert unfavorable physicochemical properties of bioactive molecules (including phytochemicals/natural products) to desirable pharmacokinetic profiles, improve delivery of therapeutics across biological barriers and compartments, reduce intrinsic cytotoxicity, control release, and enhance therapeutic efficacy by selective delivery of therapeutics to biological targets.4042 In this context, the good pharmacological activity and the physicochemical limitations such as low solubility, low permeability, and poor bioavailability, therefore make CL a suitable candidate for nanoencapsulation.15,43

Formulation of NPs

PCL-based NPs were prepared by the nanoprecipitation method and investigated with the goal to enhance its dispersity in aqueous solution and the resulting bioavailability.

As illustrated in Figure 2, the CL-loaded NPs in water provided a clear and fully dispersible formulation, with CL’s natural color intense yellow. In contrast, free CL is not dissolved in aqueous media with the visible precipitation of red crystals in the solution. This suggests that CL-NPs could represent a valid alternative to the use of toxic solubilizing agents, such as DMSO, normally required for the administration of CL.

Figure 2 Photograph of CL-loaded NPs (left), and CL raw material (right) after dispersion in water.
Abbreviations: CL, celastrol; NPs, nanoparticles.

Characterization of NPs

The prepared NPs showed an average diameter lower than 200 nm (189.1±2.9 nm and 175.5±4.7 nm for unloaded and CL-loaded NPs, respectively). Besides, as depicted in Figure 3, both NP formulations are characterized by a unimodal particle size distribution, as also confirmed by the obtained low PDIs (<0.2), typical of monodispersed systems (Table 1). Moreover, the zeta potential of the uncoated NPs was negative (−15.6±0.3 mV), as expected, without significant differences between the loaded batch (−16.2±0.4 mV), thus reflecting a steric stabilization effect that avoids aggregation of the fine particles in the colloidal system.

Figure 3 Particle size distributions and SEM images.
Note: (A) Empty and (B) CL-loaded NPs.
Abbreviations: CL, celastrol; NPs, nanoparticles; SEM, scanning electron microscopy.

Table 1 Particle size (PS), polydispersity index (PDI), zeta potential (ZP), entrapment efficiencies (EE), drug loading content (DLC), and yields of production (YPs) of formulated NPs
Note: Data are expressed as mean ± standard deviation (n=3).
Abbreviations: CL, celastrol; NPs, nanoparticles.

SEM photomicrographs revealed similar morphological aspects for both kinds of NPs (loaded and unloaded), with spherical shape and without a tendency to aggregate (Figure 3), suggesting that loading of CL into NPs did not significantly affect their size and morphology.

As far as the drug EE is concerned, the PCL demonstrated a good encapsulation capability of the CL into the polymeric matrix, with EE% values of 65.2%±1.1%, corresponding to a DLC value of 3.26%±0.1% (Table 1). This finding can be ascribed to the high affinity between the hydrophobic polymer and CL molecules. Moreover, YPs obtained were in the range of 50%.

Figure 4 shows the comparison of FTIR spectra for pure CL, unloaded and CL-loaded NPs. The CL spectrum showed a band ~2,950–2,850 cm−1 corresponding to –OH stretching, a strong characteristic peak at 1,702 cm−1 attributed to −C=O stretching, the band at 1,582–1,510 cm−1 due to C-C stretch in ring, a peak centered at 1,440 cm−1 assigned to C-H bending, and the band at 1,323–1,110 cm−1 corresponding to C–O stretch.18

Figure 4 FTIR spectra of CL raw material, unloaded NPs, and CL-loaded NPs.
Abbreviations: FTIR, Fourier transform infrared spectroscopy; CL, celastrol; NPs, nanoparticles.

According to Elzein et al44 unloaded NPs shared the characteristic peaks of PCL, with asymmetric and symmetric stretching of CH2 at 2,949 and 2,865 cm−1, respectively. Moreover, a specific signal for the carbonyl-stretching mode is indicated at 1,725 cm−1, whereas the C–O and C–C stretching at 1,293 cm−1, asymmetric COC stretching at 1,240 cm−1, and OC–O stretching at 1,190 cm−1. On the other hand, the presence of new absorption band at 1,660–1,500 cm−1 in the CL-loaded NPs’ spectrum supported the successful encapsulation of CL into nanosystems. Additionally, we observed two shifts for the carbonyl stretching to 1,727 cm−1 and for OC–O stretching to 1,186 cm−1, which might be explained by a weak interaction between the CL and the polymeric chains.

Results obtained through Raman studies (Figure 5) were in good agreement with FTIR analyses. The spectrum of unloaded NPs, containing only PCL, showed strong bands at 3,000–2,800 cm−1 and bands between 1,400 and 1,500 cm−1, which correspond to asymmetric and symmetric CH2 stretching, respectively. The peak centered at 1,725 cm−1 was characteristic of carbonyl stretching, and band at 1,293 cm−1 was attributed to C–O and C–C stretching vibrations.45,46

Figure 5 Raman spectra of CL raw material, unloaded NPs, and CL-loaded NPs.
Abbreviations: CL, celastrol; NPs, nanoparticles.

In CL spectrum, the bands at 1,650–1,500 cm−1 are due to the C=C stretching vibrations, and the intense absorption peak at 1,445 cm−1, related to the axial deformation of C=O of carboxylic acid, is also consistent with the OH deformation in carboxylic acid. Moreover, the peak registered at 1,385 cm−1 is attributed to C–H symmetric deformation of CH3. The overlapping signals corresponding to the PCL and CL, detected in CL-loaded NPs, confirmed the presence of encapsulated CL.

Additionally, DSC experiments were performed to investigate the physical state of the CL encapsulated in the NPs, as well as CL and polymer interactions. The DSC thermogram of CL (Figure 6) shows a sharp endothermic peak at 156.2°C, which corresponds to the melting point of CL, and an exothermic peak at 210.1°C, probably due to the degradation of CL.47

Figure 6 DSC curves of CL raw material, unloaded NPs, and CL-loaded NPs.
Abbreviations: DSC, differential scanning calorimeter; CL, celastrol; NPs, nanoparticles; Exo, exothermic.

The DSC scan of unloaded NPs exhibits the characteristic melting endotherm of semi-crystalline PCL at 64.55°C, shifted to 59.66°C in CL-loaded NPs thermogram, suggesting intermolecular interactions between the polymer and CL.18

Furthermore, the disappearance of the endothermic peak observed in the DSC curve of CL-loaded NPs indicates that CL was dispersed within NPs in an amorphous state, or that it is present as a solid molecular dispersion in the polymer matrix.

In vitro release studies

The highly hydrophobic nature of the CL is one of the major limiting factors for its clinical development. In order to avoid the use of toxic organic solvents, as well as to provide controlled release and protection, the nanoformulation is demonstrating to be a useful strategy.16 Figure 7 shows the release profiles of free CL and CL encapsulated in PBS, at pH 7.4. As can be noted, the amount of CL raw material dissolved in the media results are negligible (maximum 6%, after 72 hours), due to the poor solubility of the molecule. Conversely, the CL released from NPs result significantly increased, with percentages of native compound recovered of 17%, 27%, and 60% after 24, 48, and 72 hours, respectively. The obtained results demonstrated a more favorable increase of CL solubility after the encapsulation into polymeric NPs than that of liposome formulations previously reported.14

Figure 7 In vitro release profiles of free CL and CL-NPs in PBS (pH 7.4), performed for 72 hours.
Abbreviations: CL, celastrol; NPs, nanoparticles; PBS, phosphate-buffered saline.

Cytotoxicity and cellular uptake of NPs

In vitro cytotoxic activity of free CL, CL-NPs and empty-NPs was evaluated by MTT assay using androgen-responsive (LNCaP) and androgen-unresponsive (DU-145 and PC3) prostate cancer cells. More specifically, cells were incubated in presence of increasing CL-NPs concentrations (equivalent CL concentrations 0.5, 1.0, and 2.0 μM were run in parallel) for 24 hours.

On the whole, the results demonstrated that CL encapsulated in NPs was more effective against both cancer cell lines than free CL, in a dose-dependent manner (Figure 8). However, differences in sensitivity toward both CL and CL-NPs exposure were found for the tested tumor cells. In particular, in LNCaP cells no influence on viability inhibition was observed after treatment with both free CL and CL-NPs at 0.5 μM concentration, with respect to untreated cells (control) (Figure 8A). The increase of free-CL dose to 1.0 and 2.0 μM concentrations improved the anti-proliferative activity with an inhibition of cell viability by 43% and 82%, respectively ([inhibitory concentration] IC50 dose of 1.32 μM). Treatment with NPs at comparable doses of CL-NPs resulted in an enhanced reduction of cell viability (42%) at 1.0 μM concentration while a concomitant inhibition of approximately 80% was observed at 2.0 μM.

Figure 8 Viability of LNCaP (A), DU-145 (B), and PC3 (C) cells cultured with empty NPs (CL-NPs 0), and CL-loaded NPs (CL-NPs) for 24 hours, in comparison with that of free CL at the 0.5, 1.0, and 2.0 μM dose (n=3).
Notes: *Significantly different (P<0.05) from control. §Significantly different (P<0.05) from other free CL doses. °Significantly different (P<0.05) from other CL-NPs doses. #Significantly different (P<0.05) from free CL at equivalent doses.
Abbreviations: CL, celastrol; NPs, nanoparticles; Ctr, control.

Similar experiments were performed on DU-145 cells where native agent resulted in a cell viability decrease of 10%, 74%, and 88% respectively at 0.5, 1.0, and 2.0 μM concentrations, with an IC50 of 0.82 μM. CL-NPs also demonstrated a significant increase in cytotoxicity activity with 1 and 2 μM, demonstrating 62%, and 86% inhibition of cell viability, respectively, which was comparable to the native agent (Figure 8B).

Finally, at the same doses, CL-NPs showed an enhanced growth inhibition of PC3 cells of 64% and 80%, respectively, thus resulting in a significant cell viability reduction of approximately 40%, and 32%, compared with that of free CL (IC50 dose of 1.10 μM) (Figure 8C).

Similarly to LNCaP, in both DU-145 and PC3 cell lines, no significant differences were observed after treatment at 2.0 μM dose.

These results suggest that NPs enhanced the CL cytotoxicity at lower doses by consistently decreasing the androgen-independent prostate cancer cell lines’ (DU-145 and PC3) proliferation and viability, thus allowing a reduction of the CL amount administered and limiting the adverse effects.10 Importantly, no significant influence on cell viability was observed for empty NPs evaluated, considering the same concentration of the polymer as in CL-NPs at 2.0 μM, confirming the good biocompatibility of PCL polymer.

According to literature reports, the higher cytotoxicity demonstrated by CL formulated into NPs can be ascribed to the combination of different and mutual mechanisms. For example, the NPs were adsorbed on the cell surface, and this interaction generates a concentration gradient that promotes the drug influx into the cell.48 Although part of free CL molecules was actively exported out from the cell by P-gp pumps, NPs are taken up by cells through an endocytosis process, thus resulting in a consistent cellular uptake of the entrapped drug,49 which enabled them to escape from the effect of P-gp pumps. Moreover, intracellular delivery of CL-NPs leads to a massive drug accumulation and localization near to the site of action, thus allowing for higher impact in terms of efficacy, but retaining the mechanistic identity of free CL.50

The cellular uptake of FITC-loaded NPs, in all prostate cancer cell lines, was further investigated by confocal laser scanning microscope. As shown in Figure 9, after 2 hours incubation, NPs encapsulating FITC exhibited higher cellular uptake with strong fluorescence in both the cell cytoplasm and around the nucleus, confirming their internalization and sustained retention by the cancer cells. The cellular uptake efficiency in DU-145 (Figure 9B) and PC3 (Figure 9C) cell lines appeared higher than that of LNCaP (Figure 9A) cells, thus supporting the differences in sensitivity toward CL-NPs exposure observed in cytotoxicity results.

Figure 9 Confocal laser scanning microscopy images.
Notes: (A) LNCaP, (B) DU-145, and (C) PC3 cell lines, after 120 minutes incubation with FITC-loaded NPs at 37.0°C. The NPs’ internalization is observed as green fluorescence of FITC. Magnification 20×.
Abbreviations: NPs, nanoparticles; FITC, fluorescein isothiocyanate.

CL-loaded NPs suppress migration of androgen independent prostate cancer cells PC3 compared to pure CL

As the low/medium concentrations (0.5 μM and 1.0 μM) CL-NPs showed more effective results than the same concentrations of free CL in PC3 cells, we evaluated the effect of CL in comparison with CL-NPs on cell migration using the lowest (non-toxic) doses (0.5 μM) of our nanosystems. PC3 cell migration results, reported in Figure 10, demonstrated that with concentrations of CL that nominally affected proliferation (0.5 μM), CL-NPs showed more effective results in preventing cell migration (Figure 10B) than CL treatment at same concentration (Figure 10C). These data suggest that CL-NPs suppress the androgen independent prostate cancer (AIPC) cell migration at sub-cytotoxic concentration, where CL is minimally effective.

Figure 10 PC3 cell migration results.
Notes: (A) Control, (B) CL-NPs, and (C) free CL, at CL concentration of 0.5 μM.
Abbreviations: CL, celastrol; NPs, nanoparticles.

CL-loaded NPs modulate apoptosis and suppress proliferation, angiogenesis, and cell cycle protein markers in AIPC (PC3) compared to pure CL

Apoptosis is a process that comprises the activation of several key players and crucial components of the cell signaling machinery, which trigger inhibition of cell growth and proliferation. CL has demonstrated potential in basic and preclinical studies showing suppression of tumor progression through the modulation of apoptosis, inhibition of proliferation, angiogenesis, migration, cell cycle and death receptors of different tumor cell lines.10,51,52 Since CL has been shown to induce apoptosis and reduce cell viability, we next investigated the comparative effect of CL and CL-NPs on apoptosis, proliferation, angiogenesis, and cell cycle protein markers, using PC3 as a model cell line. Although CL dose-dependently induced AIPC cells’ death, CL-NPs’ effect at all instances was more potent than those of non-encapsulated CL. PC3 treated with 0.5, 1, and 2 μM of CL-NPs for 24 hours produced significantly greater fold increases in apoptosis compared with CL treated cells, as evident by significant increases in Bax/Bcl-2 ratio, cleaved PARP and decreases in survivin (Figure 11A). PARP is involved in several cellular activities such as regulation of transcription and DNA repair,53 and its inhibitors are under intense investigation in various clinical trials.54 Similarly, CL-NPs’ dose-dependent decreased proliferation (Figure 11B) and death receptors (Figure 11C) was observed by more significant reduction in Ki-67 and PCNA and TNF-R1, TNF-R2 and Fas protein expressions compared to CL alone. In addition, CL-NPs inhibited angiogenesis, as evoked by stronger PLC-γ expression (Figure 11D), and a consistently marked effect on cell cycle arrest, as supported by increases in p21 and p27 protein expression (Figure 11E), compared to the expressions induced by only CL treatment.

Figure 11 Comparative effects of pure CL and CL-NPs at various concentrations on apoptotic, proliferation, death receptor, angiogenesis, and cell cycle biomarkers.
Notes: (A) Apoptotic, (B) proliferation, (C) death receptor, (D) angiogenesis, and (E) cell cycle biomarkers. The PC3 cells were treated with each agent and harvested 24 hours after treatments. (Lane 1: control; Lane 2: unloaded NPs; Lanes 3–5: pure CL at 0.5, 1.0, and 2.0 μM concentrations, respectively; Lanes 6–8: CL-NPs at 0.5, 1.0, and 2.0 μM concentrations, respectively.
Abbreviations: CL, celastrol; NPs, nanoparticles.


Despite the potential suppression of AIPC progression, concerns related to CL bioavailability as well as perceived toxicity associated with high doses and its long-term use affect its clinical development.15,43,55 To overcome these obstacles, we have successfully developed the first formulation of CL in novel polymeric PCL NPs. The nanosystems were obtained by a nanoprecipitation technique and extensively characterized in terms of morphology, encapsulation efficiency, chemical properties, and in vitro release kinetics. The CL-NPs exhibited remarkable antiproliferative activities against all cancer cell lines compared to free CL, in a dose-dependent manner, eliciting a major sensitivity on the AIPC cell lines (DU-145 and PC3), with respect to free CL. Mechanistically, our results also showed that CL-NPs trigger a stronger cleavage of PARP, as well as induction of Bax inhibition of Bcl-2, compared to free CL and control. Moreover, a significant decrease in the expression of Ki-67, PCNA (cell proliferation markers), TNF-R1/2 and Fas (death receptor markers), PLC-γ (marker of angiogenesis), as well as induction of p21 and p27 (markers of cell cycle arrest) in lysates of PC3 cells treated with CL-NPs compared with free CL and controls, have been observed. Although these experiments have been performed in a two-dimensional culture setting, the results consistently indicate a potential of these novel polymeric nanotechnology-based PCL NPs as promising carrier to make bioavailable CL, providing a new opportunity in the treatment of prostate cancer.


The authors gratefully acknowledge the Regione Autonoma della Sardegna for financial support of grant CRP 25920, awarded to MS within the frame of “Legge regionale n. 7/2007, promozione della ricerca scientifica e dell’innovazione tecnologica in Sardegna, – Annualità 2010”, and the Mentored Research Scholar Grant (MRSG-11-019-01-CNE), awarded to IAS from the American Cancer Society (USA). The authors also thank Rahime Jashari for help with Western blot analysis, and Dr Davide Carboni for help with DSC analysis.


The authors report no conflicts of interest in this work.



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