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Nanoalumina induces apoptosis by impairing antioxidant enzyme systems in human hepatocarcinoma cells

Authors Alarifi S, Ali D, Alkahtani S

Received 2 February 2015

Accepted for publication 31 March 2015

Published 25 May 2015 Volume 2015:10(1) Pages 3751—3760

DOI https://doi.org/10.2147/IJN.S82050

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 4

Editor who approved publication: Dr Thomas J Webster

Saud Alarifi, Daoud Ali, Saad Alkahtani

Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia

Abstract: Alumina nanoparticles (Al2O3NPs) are gradually used in various areas, including nanomedicine, biosensors, and electronics. The current study aimed to explore the DNA damage and cytotoxicity due to Al2O3NPs on human hepatocarcinoma cells (HepG2). The MTT and neutral red uptake assays showed that Al2O3NPs induce significant cell death in a dose- and time-dependent manner. However, Al2O3NPs induced significant intracellular reactive oxygen species production and elevated lipid peroxidation and superoxide dismutase levels in the HepG2 cells. Al2O3NPs also induced significant decrease in reduced glutathione levels and increase caspase-3 activity in HepG2 cells. DNA fragmentation analysis using the alkaline single-cell gel electrophoresis showed that Al2O3NPs cause genotoxicity in dose- and time-dependent manner. However, they induce reactive oxygen species production and oxidative stress, leading to oxidative DNA damage, a probable mechanism of genotoxicity. This study warrants more careful assessment of Al2O3NPs before their industrial application.

Keywords: HepG2 cells, Al2O3NPs, oxidative stress, MTT assay, DNA damage
 

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