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Molecular Characterization, Drug Resistance and Virulence Analysis of Constitutive and Inducible Clindamycin Resistance Staphylococcus aureus Strains Recovered from Clinical Samples, Tehran – Iran

Authors Goudarzi M, Tayebi Z, Fazeli M, Miri M, Nasiri MJ

Received 27 February 2020

Accepted for publication 8 April 2020

Published 22 April 2020 Volume 2020:13 Pages 1155—1162


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Professor Suresh Antony

Mehdi Goudarzi,1 Zahra Tayebi,2 Maryam Fazeli,3 Mirmohammad Miri,4 Mohammad Javad Nasiri1

1Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2Microbiology Department, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran; 3Department of Virology, Pasteur Institute of Iran, Tehran, Iran; 4Department of Critical Care and Anesthesiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Correspondence: Mehdi Goudarzi
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran, Koodak-Yar St., Daneshjoo Blvd, Velenjak, Chamran HWY, Tehran, Iran
Tel/Fax +98 21 23872556

Background: Macrolide-lincosamide streptogramin B family is one of the important alternative antibiotics for treating staphylococcal infections. The aim of this study was to determine the characteristics and prevalence of antibiotic resistance genes in different coagulase types of clinical Staphylococcus aureus strains.
Methods: In the present study, 86 isolates with different phenotypes of MLSB resistance were investigated. In vitro susceptibility was assessed by the disk diffusion and broth microdilution methods. PCR assays were used to detect resistance-related genes. Coagulase and SCCmec types were identified by multiplex PCR assay.
Results: The prevalences of constitutive MLSB, inducible MLSB, and MS phenotypes were found to be 23%, 14.2%, and 4.9%, respectively. The rates of resistance to mupirocin, fusidic acid, and tigecycline were found to be 9.3%, 4.6%, and 2.3%, respectively. The top three predominant resistance genes were mecA, tet(M), erm(C) representing 75.6, 50, and 40.7% of isolates. mupA (7%), fusB (3.5%), and fusC (1.2%) genes were also detected among tested isolates. Coagulase types were mainly type II (34.9%), followed by III (32.6%), V (20.9%), and I (11.6%).
Conclusion: These findings indicated high resistance rate and low genetic variability with the prominence of coa type II, highlighting the particular importance of diagnosis of these strains to avoid treatment failure.

Keywords: Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, polymerase chain reaction, PCR, coagulase, clindamycin

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