Mechanistic studies on the absorption enhancement of a self-nanoemulsifying drug delivery system loaded with norisoboldine-phospholipid complex
Authors Zhang J, Wen X, Dai Y, Xia Y
Received 11 April 2019
Accepted for publication 9 August 2019
Published 2 September 2019 Volume 2019:14 Pages 7095—7106
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 3
Editor who approved publication: Dr Linlin Sun
Jing Zhang,1,* Xiaoxia Wen,1,* Yue Dai,2 Yufeng Xia1
1Department of Pharmacognosy, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, People’s Republic of China; 2Department of Traditional Chinese Medicine and Pharmacology, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Yufeng Xia
Department of Pharmacognosy, School of Traditional Chinese Pharmacy, China Pharmaceutical University, 639 Longmian Avenue, Jiangning District, Nanjing 211198, China
Tel +86 25 83271400
Fax +86 25 85301528
Department of Traditional Chinese Medicine and Pharmacology, School of Traditional Chinese Pharmacy, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, China
Background: Norisoboldine (NOR), the main isoquinoline alkaloid constituent in Radix Linderae, was demonstrated to have an outstanding anti-arthritis activity. However, a poor oral bioavailability of NOR creates a barrier for its development and application.
Methods: A new self-nanoemulsifying drug delivery system (SNEDDS) loaded with the phospholipid complex (PC) was designed to improve the oral bioavailability of NOR. NOR-PC was prepared by solvent evaporation method with a mixture of phospholipid and NOR at a mass ratio of 3:1. The property of PC is to improve the liposolubility of NOR, and made PC embedded in the drug delivery system. The physicochemical property of NOR-PC was characterized by differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FT-IR). According to the ability to dissolve NOR-PC, the oil and cosurfactant were chosen. The surfactant was selected based on its emulsification efficiency in SNEDDS. Pseudo-ternary phase diagram was created to select the best formulation of NOR-PC-SNEDDS, and the pharmacokinetic parameters were detected in rats. In addition, intestinal lymphatic transport and liver microsome experiment were studied to gain insight into the mechanism for NOR-PC-SNEDDS increasing the oral bioavailability of NOR.
Results: Solubility detection showed that the PC significantly improved the liposolubility of NOR. NOR-PC-SNEDDS was prepared using NOR-PC, Ethyl oleate, Labrasol, Cremophor EL and transcutol HP at a weight ratio of 1:2:3.36:2.24:2.4 (w/w/w/w/w). The particle size and zeta potential of NOR-PC-SNEDDS were 36.72±1.47 nm and −4.91±0.49 mV after dilution with distilled water at a ratio of 1:50 (w/w). The absolute bioavailability of NOR in the NOR-PC-SNEDDS group significantly increased and the value was 372% in relative to NOR group. Further studies indicated that NOR-PC-SNEDDS promoted the oral bioavailability of NOR by enhancing intestinal lymphatic absorption and inhibiting Phase II metabolism of NOR.
Conclusion: These findings suggested that NOR-PC-SNEDDS was able to promote the oral bioavailability of NOR, which provided a foundation for the further development and application of NOR.
Keywords: norisoboldine, oral bioavailability, self-nanoemulsifying drug delivery system, lymphatic absorption, Phase II metabolism
Rheumatoid arthritis (RA) is a common systemic autoimmune disease characterized by chronic synovial inflammation and hyperplasia as well as joint destruction and ultimate disability. Epidemiologic studies show that RA may increase the risk of comorbidities, such as cardiovascular disease, osteoporotic fracture and malignancy, and results in significant mortality. The prevalence of RA is estimated to be about 1% of the global population and 0.42% of the population in People's Republic of China.1,2 Currently, the disease-modifying anti-rheumatic drugs, non-steroidal anti-inflammatory drugs, and glucocorticoid are widely used for the treatment of RA. But their clinical applications were limited by the annoying side effects and the high costs. Therefore, there is an urgent need to find a novel candidate agent for the treatment of RA.
Norisoboldine (NOR, Figure 1) is an isoquinoline alkaloid isolated from the dry root of Lindera aggregate (Sims) Kosterm, which exhibited outstanding anti-arthritis activity.3–5 Previous studies in our laboratory showed that NOR (15 and 30 mg/kg, oral) significantly decreased the swelling of paws and arthritis index scores, and rescued the body weight loss of mice with collagen-induced arthritis.4 The anti-arthritic mechanism of NOR involved the restoration of the balance between Th17 and regulatory T cells.6 Furthermore, synovial angiogenesis is considered as a potential target for the treatment of RA. Our findings demonstrated that NOR was able to prevent synovial angiogenesis in rats by moderating the Notch1 pathway-related endothelial tip cell phenotype with a potential action target of the Notch1 transcription complex.7,8 Therefore, NOR might become a promising candidate drug for the treatment of RA in view of the action mode of multiple targets and low toxic side effects. However, pharmacokinetic studies showed that the oral bioavailability of NOR was only 2.77%,9 which limit the further development of NOR.
Figure 1 Chemical structure of NOR. Abbreviation: NOR, norisoboldine.
Self-nanoemulsifying drug delivery system (SNEDDS) has been commonly used to improve the bioavailability of insoluble drugs.10–12 SNEDDS is a uniform and transparent solution composed of drug, oil, surfactant, and cosurfactant. SNEDDS can spontaneously form isotropic, transparent,or translucent solution in water with mild agitation at ambient temperature (usually at 37°C). SNEDDS improves the oral bioavailability of the drugs by increasing solubility, accelerating intestinal permeability, facilitating intestinal lymphatic absorption, and attenuating gastrointestinal metabolism.13–15 Notably, NOR is unable to be embedded in SNEDDS without any treatment due to the low liposolubility. Previous reports indicated that phospholipid complex (PC) technique could improve the liposolubility of water-soluble drugs, and made them embedded in the drug delivery system.16–18 Therefore, NOR-PC was used to improve the liposolubility of NOR and prepared into NOR-PC-SNEDDS in combination with optimized excipients of SNEDDS.
To sum up, the aim of the present study was to design a new SNEDDS loaded with the PC to improve the oral bioavailability of NOR. Pharmacokinetic comparisons of the NOR, NOR-PC, and NOR-PC-SNEDDS were performed, and the oral bioavailability of NOR-PC-SNEDDS was investigated. Furthermore, the mechanism for the absorption enhancement of NOR-PC-SNEDDS was explored.
Materials and methods
NOR (purity >98%) was purchased from Chengdu Chroma Biotechnology Co., Ltd. (Chengdu, People's Republic of China). soybean phospholipid (95% phosphatidylcholine) was donated by Shanghai Avet Pharmaceutical Technology Co., Ltd. (Shanghai, People's Republic of China). Labrafac Lipophile WL1349, Labrafil M 1944 CS, Peceol, Capryol 90, Lauroylycol 90, Labrasol, and Transcutol HP (analytical purity) were kindly supplied by Gattefossé (Shanghai, People's Republic of China). Cremophor EL, Cremophor RH 40, and Solutol HS 15 (analytical purity) were donated by BASF (Shanghai, People's Republic of China). Oleic acid and tween 80 (analytical purity) were purchased from Nanjing Chemical Reagent Co., Ltd. (Nanjing, People's Republic of China). Ethyl oleate and MgCl2 (analytical purity) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, People's Republic of China). UDPGA (purity >93%), D-Saccharic acid 1, 4-lactone (purity >98%), and alamethicin (purity >98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). BCA Protein Assay Kit was purchased from Nanjing Jiancheng Bioengineering Insitute (Nanjing, People's Republic of China). Sinomenine (purity >98%, internal standard) was purchased from Nanjing Jingzhu Bio-technology Co., Ltd. HPLC-grade acetonitrile was purchased from Merck (Darmstadt, German). All other chemicals and solvents were of analytical purity.
Sprague-Dawley rats (180–220 g) were obtained from Nanjing Qinglongshan Animal Breeding Center (Nanjing, People's Republic of China). Rats were housed under controlled conditions of temperature (22±2°C) and relative humidity (50±10%) with a 12-hr light/dark cycle. All rats had free access to food and sterile water for 7 days. All animal experiments were performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals, with the approval of the Animal Ethics Committee of School of Traditional Chinese Pharmacy, China Pharmaceutical University. Before experiments, rats were fasted for 12 hrs while water was taken freely.
Preparation of NOR-PC
Soybean phospholipid and NOR at a mass ratio of 3:1 were mixed to prepare NOR-PC. Required amounts of NOR and phospholipids were placed in a 100 mL round-bottom flask and dissolved in absolute ethanol. The concentration of NOR was 1.25 mg/mL. The mixture was then stirred with a magnetic mixer at 40°C for 3 hrs. Afterward, a vacuum rotary evaporator was used to evaporate solvent, and the residue was dried under vacuum for 24 hrs. The resultant NOR-PC was transferred into a glass bottle filled with nitrogen and stored at room temperature.
Characterization of NOR-PC
The physicochemical property of NOR-PC was characterized by differential scanning calorimetry (DSC) and Fourier transform infrared spectrophotometry (FT-IR). DSC analyses of NOR, phospholipid, NOR-PC, and physical mixture of NOR and phospholipid were recorded using a Netzsch DSC 204 F1 phoenix differential scanning calorimeter (Netzsch, Selb, Germany), respectively. The samples were heated from 40°C to 300°C at a heating rate of 10°C/min. The FT-IR spectra of the examined samples were conducted in the range of 4000–400 cm−1 with a resolution of 4 cm−1 using a Bruker tensor 27 FT-IR spectrometer (Bruker Corporation, Billerica, MA, USA). The examined samples were obtained employing compressed disks of appropriate amount of NOR, phospholipid, NOR-PC, and physical mixture of NOR and phospholipid separately mixed with KBr in the dry environment.
Determination of NOR content in NOR-PC
HPLC method was established to determine the concentration of NOR in NOR-PC. HPLC analysis was carried out on a Shimadzu LC-2010AHT HPLC system equipped with an ODS-C18 column (150 mm×6 mm, 5 μm) (Shimpack Technologies, Kyoto, Japan). The mobile phase was acetonitrile:0.1% formic acid water (15:85, v/v) at a flow rate of 1 mL/min and the injection volume was 20 μL. Column temperature and detection wavelength were set at 30°C and 280 nm, respectively.
The solubility of NOR-PC in double distilled water and n-octanol was measured to check whether the liposolubility of NOR was increased after the preparation of NOR-PC. Excess NOR, NOR-PC, and physical mixture of NOR and phospholipids were separately added to 5 mL double distilled water or n-octanol. The samples were shaken at 25±2°C for 24 hrs and then centrifuged at 12,000 rpm for 20 mins. Then, the supernatants were taken and diluted with methanol:0.1% formic acid water (3:2, v/v). The concentration of NOR was determined by the above HPLC method.
The solubility of NOR-PC in different oil phases, surfactants, and co-surfactants were determined to select proper formulation of NOR-PC-SNEDDS. One milliliter of various oil phases, surfactants, and co-surfactants were accurately measured and mixed with excess NOR-PC or NOR. The mixture was vortex-mixed for 1 min and shaken for 48 hrs at 37°C. The samples were centrifuged at 12,000 rpm for 20 mins. The supernatant (10 μL) was collected and diluted with 990 μL hydrochloric acid-methanol (0.5%, v/v). The mixture was vortex-mixed for 1 min and determined by HPLC method.
Construction of pseudo-ternary phase diagram
Ethyl oleate was selected as the oil phase, Labrasol:Cremophor EL (3:2) as the surfactant and Transcutol HP as the cosurfactant according to the results of the preliminary experiments. The amount of oil phase was fixed at 10–90% (w/w) and the ratio of surfactant to co-surfactant was from 1:9 to 9:1 to screen prescriptions. The mixture was stirred with a magnetic agitator for 20 mins. NOR-PC was added to the drug-loaded prescriptions before mixture.
Twenty-five milliliters of 0.1 M HCl solution was stirred with a magnetic agitator at 37°C for a low speed. A quantity of 100 μL of the above-mixed solution was added to form a self-emulsified area with transparent or microstrip blue opalescence. Analysis software Origin 8.0 was used to construct the pseudo-ternary phase diagrams.
Characterization of NOR-PC-SNEDDS
The particle size, percent polydispersity, and zeta potential of the optimized NOR-PC-SNEDDS after dilution with distilled water (1:50, v/v) were measured by a Malvern Zetasizer Nano ZS90 (Malvern Instruments, UK). Each sample was assayed for three times.
The morphological features of the NOR-PC-SNEDDS were observed by a Hitachi HT7700 transmission electron microscope (Hitachi Ltd.). The NOR-PC-SNEDDS was diluted with distilled water (1:50, v/v) and mixed with vortex. One drop of the sample was placed on the copper sheet and negatively stained by 2% phosphomolybdic acid for 30 s prior to observation.
Thermodynamic stability of NOR-PC-SNEDDS
The effects of different media and dilution factors on the particle size and polydispersity index (PDI) of NOR-PC-SNEDDS were investigated, respectively. NOR-PC-SNEDDS was diluted with distilled water, pH 1.2 HCl solution (0.1 M), and pH 6.8 phosphate buffer to 50, 100, and 1000 folds at 37°C, respectively.16,19 Then, the particle size and PDI were determined using a Malvern Zetasizer Nano ZS90 to investigate the stability.
The effects of freeze–thaw on particle size and PDI were further investigated. After NOR-PC-SNEDDS was chilled for 24 hrs at −20°C, it was placed at room temperature for 24 hrs, then freezed and thawed repeatedly for three times. Next, it was diluted 250 folds with distilled water. The particle size and PDI were measured to examine the thermodynamic stability.
Pharmacokinetic studies of NOR in rats
Rats were randomly divided into four groups (five rats in each group), including NOR group, NOR-PC group, NOR-PC-SNEDDS group, and NOR-IV group. The first three groups were administered by gavage at a dose equivalent to 30 mg/kg of NOR. Blood samples (300 μL) were collected into heparinized tubes via the oculi choroidea vein at 0, 0.083, 0.17, 0.33, 0.5, 0.75, 1, 1.5, 2, 3, 4, 8, 12, and 24 hrs after oral administration. NOR-IV group was administered by tail vein injection at a dose equivalent to 2 mg/kg of NOR. Blood samples (300 μL) were collected from the oculi choroidea veins at 0, 0.033, 0.083, 0.17, 0.33, 0.5, 0.75, 1, 1.5, 2, 3, 4, 8, 12, and 24 hrs. The obtained blood samples were centrifuged immediately at 8000 rpm for 10 mins at 4°C, and stored at −20°C until analysis.
The plasma samples were thawed at room temperature prior to processing. A 100 μL of plasma sample was transferred to an Eppendorf tube, and 40 μL of IS (300 ng/mL) was spiked and mixed for 30 s. Then, 460 μL methanol was added, and the mixture was thoroughly vortexed. After centrifugation at 12,000 rpm for 10 mins, the supernatant (400 μL) was transferred to a centrifuge tube and evaporated to dryness in a vacuum concentrator at 37°C. The obtained residue was reconstituted in 50 μL 0.1% formic acid water and centrifuged at 12,000 rpm for 10 mins. The supernatant was used for LC–MS/MS analysis.
The samples were analyzed using an Agilent 1260 liquid chromatography (Agilent Technologies, Santa Clara, CA, USA) with an Agilent 6420 triple quadrupole mass spectrometer (Agilent Technologies). Chromatographic separation was performed on a ZORBAX Extend-C18 column (2.1×50 mm, 1.8 μm) (Agilent Technologies). The mobile phase consisting of acetonitrile:0.1% formic acid water (9:91, v/v) was delivered at a flow rate of 0.2 mL/min. The injection volume was 5 μL. The electrospray ionization-MS/MS operation parameters were set as follows: positive ion electrospray; drying gas (N2) temperature, 300°C; drying gas (N2) flow rate, 11 L/min; capillary voltage, 4.0 kV; nebulizer pressure, 35 psig; fragmentor voltage, 100 V for NOR and IS; and collision energy, 15 eV for NOR and 35 eV for IS. Multiple-reaction monitoring used the transitions of m/z 314→265 for NOR and m/z 330→181 for IS.
Intestinal lymphatic absorption
Rats were divided randomly into two groups (five rats in each group), and fasted for 12 hrs but granted free access to water prior to the experiment. One group was only orally administered with NOR-PC-SNEDDS (30 mg/kg). Another group was intraperitoneally injected with cycloheximide (CYC) (3 mg/kg) which was prepared with 0.9% physiological saline 90 mins before oral administration of NOR-PC-SNEDDS (30 mg/kg).
After administration, blood samples (300 μL) were collected from the oculi chorioidea veins at 0, 0.083, 0.17, 0.33, 0.5, 0.75, 1, 1.5, 2, 3, 4, 8, 12, and 24 hrs. The supernatant (100 μL) was collected after centrifugation (8000 rpm, 4°C, 10 mins) and stored at −20°C until analysis.
The method of the sample pretreatment and the condition parameters of HPLC were the same as the previous experiment.
Comparison of the metabolism of NOR-PC-SNEDDS and NOR in rat liver microsomes
Rat liver microsomes were prepared by differential centrifugation.20 The microsomal protein concentration was measured using a BCA protein assay kit.
The incubation mixture consisted of varying concentrations of NOR (5, 10, 25, or 50 μM) or NOR-PC-SNEDDS (calculated according to the percentage of NOR in NOR-PC-SNEDDS), rat liver microsomes (0.5 mg protein/mL), 2 mM UDPGA, alamethicin (25 μg/mL), 5 mM D-saccharic acid 1,4-lactone, and 10 mM MgCl2. Then, the mixture was replenished with Tris-HCl buffer (pH 8.0) at a volume of 200 μL. After pre-incubation for 3 mins at 37°C, UDPGA was added to initiate the reaction. After incubation for 30 mins, 1 mL ice ethyl acetate containing 10 μL IS (sinomenine, 0.56 mM) was added to terminate the reaction. The mixture was vortex-shaking for 3 mins and centrifuged at 12,000 rpm for 10 mins. The supernatant (800 μL) was transferred to a centrifuge tube and evaporated to dryness in a vacuum concentrator at 40°C. The obtained residue was reconstituted in 100 μL 0.1% formic acid water and centrifuged at 12,000 rpm for 10 mins. The supernatant was used for HPLC analysis. The incubation system without UDPGA was used as a control group. All experiments were conducted in triplicate.
The effects of other components of NOR-PC-SNEDDS on the metabolism of NOR were further investigated. A quantity of 50 μM NOR and a series of concentrations (0.01, 0.1, 1 mg/mL) of Cremophor EL, Labrasol, and Transcutol HP were added into the incubation system, respectively. The metabolic amount of NOR in every group was determined using HPLC after incubation for 30 mins. The NOR incubation group without the excipients was used as the negative control group and the metabolic amount of this group was set at 100% as a reference. The residual activity of the NOR in the Cremophor EL, Labrasol, and Transcutol HP groups was calculated, respectively.
The residue of NOR was determined using an Agilent 1260 liquid chromatography and a Shimpack CLC-ODS column (150 mm×6 mm, 5 μm). The mobile phase consisting of methanol-0.1% formic acid water (15:85, v/v) was delivered at a flow rate of 1 mL/min. The column temperature was maintained at 40°C. The injection volume was 20 μL, and the detection wavelength was set at 280 nm.
PK Solver (Version 4; Thermo Fisher Scientific, Waltham, MA, USA) was used to calculate various pharmacokinetic parameters, including the maximum plasma concentration (Cmax), time to maximum plasma concentration (Tmax), area under the plasma concentration-time curve (AUC), and clearance rate (Cl/F) according to the non-compartment model. The experimental data were expressed as mean±SD and analyzed with statistical analysis software SPSS (SPSS Inc., Chicago, IL, USA). Statistical differences were assessed by one-way ANOVA in combination with independent-sample t-test. A value of P<0.05 was considered statistically significant.
Results and discussion
Representative chromatograms are shown in Figure 2. Under the chromatographic conditions described, the retention time of NOR was 7.8 mins. After fully validated, the method was successfully applied to determine the concentration of NOR in NOR-PC.
Figure 2 HPLC chromatograms of NOR. Notes: (A) Blank sample. (B) Blank sample spike with NOR. (C) NOR-PC. Abbreviations: NOR, norisoboldine; NOR-PC, norisoboldine-phospholipid complex.
Characterization of NOR-PC
DSC was used to study the possible interaction between NOR and phospholipids. NOR had two endothermic peaks at 132°C and 194°C as shown in Figure 3D. Thermogram of phospholipids (Figure 3C) showed a sharp endothermic peak at 236°C which was the transition from gel state to liquid crystal state. This might be the melting of phospholipid hydrocarbon chain, isomerization, or crystal form change.18 Both the peaks of NOR and phospholipid disappeared in the DSC curve of NOR-PC (Figure 3A). The result indicated that NOR was dissolved completely in the soybean phospholipid and the interactions between NOR and phospholipid occurred. The interactions between NOR and PC contributed to entrapment of the NOR into the drug-loading system and formation of the NOR-PC. Thermogram of the mixture of NOR and phospholipids showed an endothermic peak of NOR-PC 160°C and an endothermic peak of NOR 135°C (Figure 3B), which might be due to the melting of soybean phospholipids and the interaction with the NOR during the heating process.
Figure 3 DSC thermograms. Notes: (A) NOR-PC. (B) Physical mixture of NOR and phospholipids. (C) Phospholipids. (D). NOR.Abbreviations: DSC, differential scanning calorimetry; NOR-PC, norisoboldine-phospholipid complex; NOR, norisoboldine.
In the phospholipid FT-IR spectrum (Figure 4C), 1235.8 cm−1 was the stretching vibration peak of P=O, 1087.8 cm−1 was the stretching vibration peak of P-O-C, and 968.3 cm−1 was the stretching vibration peak of N+(CH3)3.21,22 FT-IR spectrum of the physical mixture of NOR and phospholipids (Figure 4B) was in accordance with NOR-PC (Figure 4A) which was almost the superposition of the FT-IR spectrum of the NOR (Figure 4D) and phospholipids. The main absorption peaks of NOR and phospholipid were observed. Compared with the FT-IR spectrums of NOR and phospholipid, both absorption peaks of NOR at 1586.7 cm−1 (for C=C stretch vibration) and phospholipids at 968.3 cm−1 (for N+(CH3)3 stretch vibration) were reduced. There might be hydrogen bonds or van der Waals forces between NOR and soybean phospholipids. Due to the presence of hydroxyl groups on the benzene ring of the drug, hydrogen bonds or van der Waals forces may form between the drug and the phospholipid, which make it easier to be embedded in the drug delivery system.
Figure 4 Infrared spectra. Notes: (A) NOR-PC. (B) Physical mixture of NOR and phospholipids. (C) Phospholipids. (D). NOR.Abbreviations: NOR-PC, norisoboldine-phospholipid complex; NOR, norisoboldine.
The apparent solubilities of NOR, the physical mixture of NOR, and phospholipid as well as NOR-PC in double distilled water and n-octanol are shown in Table 1, respectively. The liposolubility of NOR in double distilled water and n-octanol increased significantly after the preparation of NOR-PC.
Table 1 Solubilities of NOR, physical mixture, and NOR-PC in water and n-octanol
Selection of oil phase, surfactant, and cosurfactant
The solubilities of NOR and NOR-PC in various oil phases, surfactants, and cosurfactants are shown in Figure 5. The solubility of NOR-PC was higher than NOR in various carriers. Among all kinds of oil phases and cosurfactants, the solubility of NOR-PC was the highest in Capryol 90 and Transcutol HP. Therefore, Capryol 90 and Transcutol HP were chosen as the oil phase and the cosurfactant to make the prescription have a larger drug loading. As shown in Figure 5, Labrasol had the highest solubility among surfactants, but its emulsifying ability was weak. Cremophor EL had the strongest emulsifying ability among surfactants. Based on pre-experiment results, the ratio of Labrasol and Cremophor EL to be 3:2 was the best of all ratios. Therefore, Labrasol:Cremophor EL (3:2) was chosen as surfactant to ensure a larger drug loading and stronger emulsifying ability. However, when Capryol 90, Labrasol:Cremophor EL (3:2), and Transcutol HP were selected as the oil, surfactant, and cosurfactant, which was difficult to emulsify, and large oil droplets were observed on the liquid surface. Therefore, the oil phase was altered to Ethyl oleate to improve self-emulsification. Finally, Ethyl oleate, Labrasol:Cremophor EL (3:2), and Transcutol HP were selected as the oil, surfactant, and cosurfactant, respectively.
Figure 5 Solubility of NOR-PC and NOR in various carriers. Abbreviations: NOR-PC, norisoboldine-phospholipid complex; NOR, norisoboldine.
Construction of pseudo-ternary phase diagram
The pseudo-ternary phase diagram can be used to screen the appropriate prescription ratio and drug loading to get the balance between drug loading and self-emulsifying efficiency in the preparation of SNEDDS. Ethyl oleate, Labrasol:Cremophor EL (3:2), and Transcutol HP were selected as the oil, surfactant, and cosurfactant, respectively. The oil phase, surfactant, and cosurfactant were used as the three vertices of the pseudo-ternary phase diagrams, respectively. Origin 8.0 software was used to get blank pseudo-ternary phase diagram. The NOR-PC was added to the blank prescription one by one. Finally, the content of NOR was 1% and the drug-loaded pseudo-ternary phase diagram is shown in Figure 6. The final formulation of NOR-PC-SNEDDS was NOR-PC/Ethyl oleate/Labrasol/Cremophor EL/Transcutol HP=1:2:3.36:2.24:2.4 (w/w/w/w).
Characterization of NOR-PC-SNEDDS
After dilution with distilled water at a ratio of 1:50 (w/w), the particle size, polydispersity coefficient, and zeta potential of NOR-PC-SNEDDS were 36.72±1.47 nm, 0.09±0.02, and −4.91±0.49 mV, respectively. The particle size and zeta potential of the emulsion droplets are important indicators for evaluating the self-emulsification properties because they determine the rate and extent of drug release as well as the stability of the emulsion. Studies have shown that small particle size is beneficial for bioavailability.23,24 In addition, the zeta potential also affects the stability of SNEDDS. High zeta potential is conducive to system stability.25
The transmission electron micrograph of NOR-PC-SNEDDS is shown in Figure 7. It could be seen that NOR-PC-SNEDDS dispersed uniformly in aqueous solution and in globular form with a narrow particle size change.
Figure 7 Transmission electron microscope image of NOR-PC-SNEDDS formulation (×20,000 magnification). Abbreviations: SNEDDS, self-nanoemulsifying drug delivery system; NOR-PC, norisoboldine-phospholipid complex.
Thermodynamic stability of NOR-PC-SNEDDS
The effects of different media, dilution factors, and freeze–thaw on particle size and PDI are shown in Table 2. The particle size and PDI of NOR-PC-SNEDDS were stable after dilution by 50, 250, and 1000 times in double distilled water, 0.1 M HCl solution (pH 1.2) and phosphate buffer solution (pH 6.8), respectively. Moreover, the particle size and PDI were stable after repeated freezing and thawing for three times.
Table 2 Thermodynamic stability of NOR-PC-SNEDDS
The pharmacokinetic characteristics of NOR in rats after oral administration of NOR, NOR-PC, NOR-PC-SNEDDS, or intravenous injection of NOR were compared. The plasma concentration-time curves of NOR in four groups are shown in Figure 8. The main pharmacokinetic parameters and the absolute bioavailability of NOR are listed in Table 3.
Table 3 Bioavailability and pharmacokinetic parameters of NOR after oral administration of NOR, NOR-PC, NOR-PC-SNEDDS, and tail vein injection of NOR
The results showed that the pharmacokinetic parameters of NOR-PC-SNEDDS group were significantly different from those of NOR and NOR-PC groups. Compared with rats in NOR group, rats in NOR-PC-SNEDDS group showed an increase of 2.67, 3.67, and 3.72 folds in T1/2, AUC0-t, and AUC0-∞ values, and a decrease of Cl/F value to 27.78%. Similarly, compared with NOR-PC group, the T1/2, AUC0-t, and AUC0-∞ values in rats of NOR-PC-SNEDDS group were increased to 2.29, 2.73, and 2.75 folds, and the Cl/F decreased to 37.04%. Furthermore, the absolute bioavailability of NOR in the NOR-PC-SNEDDS group was found to be significantly increased and the value was 372% and 275% in relative to NOR group and NOR-PC group, respectively. However, the pharmacokinetic parameters in NOR-PC group were not markedly different from those of the NOR group. The results implied that certain compositions in excipients of NOR-PC-SNEDDS might improve the bioavailability of NOR rather than phospholipid.
Intestinal lymphatic absorption
Table 4 Pharmacokinetic parameters of NOR-PC-SNEDDS group and NOR-PC-SNEDDS-CYC group
The pharmacokinetic parameters of the NOR-PC-SNEDDS-CYC group were significantly different from those of NOR-PC-SNEDDS group. Compared with NOR-PC-SNEDDS group, the Tmax, Cmax, AUC0-t, and AUC0-∞ values of NOR-PC-SNEDDS-CYC group were found to be significantly decreased to 29.63%, 29.61%, 49.04%, and 60.53%, respectively. Meanwhile, Cl/F value for the NOR-PC-SNEDDS-CYC group increased to 1.7 folds of that of the NOR-PC-SNEDDS group.
The intestinal lymphatic transport is an important way to enhance the absorption of lipophilic drugs. To investigate the lymphatic transport of drugs, a simplified and effective in vivo model named chylomicron flow blocking approach was commonly carried out.26 Chylomicrons are very large, spherical particles, and mainly composed of triglyceride (85–92%). The chylomicrons are formed and secreted by Golgi apparatus and selectively enter the lymph vessel. Drugs entering the lymphatic system need to bind to triglycerides in the chylomicrons.27 Cycloheximide, a non-specific protein synthesis inhibitor that inhibits the secretion of chylomicron from the enterocyte, can block the lymphatic transport and therefore result in the absorption decrease of drugs. In the present study, the results demonstrated that cycloheximide blocked the lymphatic transport of SNEDDS formulation, thereby reduced the absorption of SNEDDS formulation.
It has been reported that unsaturated long-chain fatty acids, such as oleic acid, peceol, maisine 35–1, have the ability to enhance the systemic bioavailability by the intestinal lymphatic transport.28 Therefore, Ethyl oleate in NOR-PC-SNEDDS prescription might contribute to promote the absorption of NOR in NOR-PC-SNEDDS via lymphatic transport and increase the bioavailability.
Comparison of the metabolism of NOR-PC-SNEDDS and NOR in rat liver microsomes
As shown in Figure 10, the metabolic rates of NOR in the series of concentrations of NOR-PC-SNEDDS groups decreased compared with NOR group. The difference was more significant following the increase of the concentration of NOR.
The effect of components of NOR-PC-SNEDDS on the metabolism of NOR Phase II was further investigated. The inhibition of Cremophor EL, Labrasol, and Transcutol HP on NOR Phase II metabolism is shown in Figure 11. Transcutol HP had no significant effect on NOR Phase II metabolism. However, Cremophor EL and Labrasol had a certain inhibitory effect on NOR Phase II metabolism and the inhibition was more obvious with concentration increasing.
Figure 11 Effects of composition of NOR-PC-SNEDDS on NOR Phase II metabolism. Abbreviations: SNEDDS, self-nanoemulsifying drug delivery system; NOR-PC, norisoboldine-phospholipid complex; NOR, norisoboldine.
UGTs (UDP-glucuronosyltransferases) are important Phase II metabolic enzymes in organisms. UGTs can bind glucuronic acid on the functional groups of compounds, increase water solubility of compounds, make compounds lose biological activity and elimination from the body. Previous study has shown that NOR was mainly metabolized to NOR-9-O-glucuronide by UGTs.29 Therefore, our results indicated the excipient components of NOR-PC-SNEDDS reduce the metabolism of NOR and improve the bioavailability.
This study designed and evaluated a novel NOR-PC-SNEDDS which improved the oral bioavailability of NOR. NOR-PC-SNEDDS functions through enhancing intestinal lymphatic transport and inhibiting the liver metabolism of NOR. The findings in this paper provide a new idea for the further development and application of NOR.
This work was supported by the China National Natural Science Foundation (No. 81872881; 81373426) and the “Double First-Class” University project (CPU2018GY10).
The authors report no conflicts of interest in this work.
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